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IL-17/IL-17A Antibodies

We offer IL-17/IL-17A Antibodies for use in common research applications: Blocking/Neutralizing, Competitive ELISA, ELISA, ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), ELISA Standard (Matched Pair), Flow (Intracellular), Flow Cytometry, Functional, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Intracellular Staining by Flow Cytometry, Neutralization, Peptide ELISA, Sandwich ELISA, Western Blot. Each IL-17/IL-17A Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our IL-17/IL-17A Antibodies can be used in a variety of model species: Canine, Human, Mouse, Primate, Rat. Use the list below to choose the IL-17/IL-17A Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our IL-17/IL-17A polyclonal antibodies and browse our IL-17/IL-17A monoclonal antibody catalog.

Alternate Names for IL-17/IL-17A Antibodies

anti-IL-17/IL-17A antibody, anti-IL17A antibody, anti-CTLA8cytotoxic T-lymphocyte-associated serine esterase 8 antibody, anti-Cytotoxic T-lymphocyte-associated antigen 8 antibody, anti-IL-17Acytotoxic T-lymphocyte-associated protein 8 antibody, anti-IL-17CTLA-8 antibody, anti-IL17interleukin-17A antibody, anti-interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8) antibody, anti-interleukin 17A antibody

49 Results for "il-17-il-17a" in Primary Antibodies

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MAB421

IL-17/IL-17A Antibody (50104) [Unconjugated]

(121 Publications)

100 μg

MAB421

Western blot shows 25 ng of Recombinant Mouse IL-17/ IL-17A (Catalog # 421-ML), Recombinant Human IL-17/IL-17A (Catalog # 317-ILB), Recombinant Rat IL-17/IL-17A (Catalog # 8410-IL), and Recombinant Mouse IL-17F (Catalog # 2057-IL). PVDF Membrane was probed with 1 µg/mL of Rat Anti-Mouse IL-17/ IL-17A Monoclonal Antibody (Catalog # MAB421) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-17/IL-17A at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Recombinant Mouse IL‑17 (Catalog # 421-ML) stimulates IL‑6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line), as measured by the Mouse IL‑6 Quantikine ELISA Kit (Catalog # M6000B). IL‑6 secretion elicited by Recombinant Mouse IL‑17 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑17 Monoclonal Antibody (Catalog # MAB421). The ND50 is typically0.05-0.15 µg/mL.

Ear histology in WT and Pglyrp-deficient mice in atopic dermatitis and contact dermatitis models of skin inflammation.(A) Oxazolone model of atopic dermatitis: sensitization and 10 applications of oxazolone to the ears every other day induced acanthosis (Ac), parakeratosis (Pk), marked thickening of the sub-epidermal layer with spongiosis (Sp) and dense cellular infiltrates of primarily mononuclear and some polymorphonuclear cells (high magnification insets), that were all highly prominent in Pglyrp3−/− mice and Pglyrp4−/− mice and much less severe in WT mice. (B) Oxazolone model of contact dermatitis: sensitization and a single application of oxazolone to the ears induced strong inflammatory response in WT mice with marked spongiosis of the sub-epidermal layer (Sp) and cellular infiltrates of epidermal and sub-epidermal layers, composed of mononuclear and polymorphonuclear cells; Pglyrp1−/− and Pglyrp1−/−Pglyrp2−/− mice still had cellular infiltrates, but had substantially reduced swelling, compared to WT mice, mostly due to reduced edema. H&E stained cross-sections; bar = 200 µm for all panels, except high magnification insets (the magnified areas are shown by rectangles). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21949809), licensed under a CC-BY license. Not internally tested by R&D Systems.

IL-17 is required for enhanced response to oxazolone in Pglyrp3−/− mice.(A) The level of IL-17-induced chemokine, CXCL-1, is higher in the ears of Pglyrp3−/− mice than WT mice after sensitization and application of oxazolone for 20 days. (B) Ear swelling in Pglyrp3−/− mice sensitized and treated 7 times with oxazolone every other day and also treated with neutralizing anti-IL-17 mAbs is lower than in Pglyrp3−/−mice similarly treated with oxazolone and isotype control IgG. Means ± SEM; N = 6 mice/group; significance of differences between Pglyrp3−/− and WT mice (A) or IgG control and anti-IL-17 mAbs-treated mice (B): *, P<0.05; **, P<0.005. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21949809), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Species Glossary

Reactivity: Mu

Applications:

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB421-SP
Carrier Free	MAB421-500

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AF-317-NA

IL-17/IL-17A Antibody [Unconjugated]

(4 Reviews)

(96 Publications)

100 μg

AF-317-NA

Western blot shows lysates of human primary naïve CD4+ T cells, along with whole cell lysates (WCL) and conditioned-media supernatant (Supe) of human primary differentiated Th17 cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for IL-17 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IL‑17/IL‑17A was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL‑17/IL‑17A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Human IL-17 was immunoprecipitated from 100 µg of human primary differentiated Th17 cell lysate following incubation with 3 µg Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA) or control antibody (AB-108-C) overnight at 4 °C. IL-17-antibody complexes were absorbed using Protein G Sepharose. Immunoprecipitated IL-17 was detected by Western blot using 1 µg/mL Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA). View our recommended buffer recipes for immunoprecipitation.

Human peripheral blood mononuclear cells were unstimulated (light orange filled histogram) or treated with 50 ng/mL PMA and 250 ng/mL Ca2+ ionomycin for 16 hours, then stained with Goat Anti-Human IL‑17 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF‑317‑NA, dark orange filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Recombinant Human IL‑17 (Catalog # 317-ILB) stimulates IL‑6 secretion in NIH/3T3 mouse embryonic fibroblasts in a dose-dependent manner (orange line), as measured by the mouse IL‑6 Quantikine ELISA Kit (Catalog # M6000B). IL‑6 secretion elicited by Recombinant Human IL‑17 (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA). The ND50 is typically0.02‑0.12 µg/mL.

Expression of psoriasis‐related cytokines in paradoxical psoriasiform reactions. Immunohistochemistry analysis performed on paradoxical skin lesions obtained from patients 1 (Pt1) and 2 (Pt2) shows similar values of IL‐17A+ cells, a reduction of dermal IFN‐ gamma + cells and an increase of IL‐22+ or IL‐36 gamma + cells, when compared with psoriatic skin lesions. LS and NLS skin of the same psoriatic patient (n = 3) was analyzed. Graphs show the mean of number of positive cells + SD per three sections. One out of three representative stainings is shown. *p < 0.01, **p < 0.05, versus classical psoriasis. Scale bars, 200 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31577850), licensed under a CC-BY license. Not internally tested by R&D Systems.

Inflammatory cell infiltrates of early acne lesions in immunohistochemistry.Representative immunohistochemical staining showing IL-17A+ cells in early acne lesion (a). Staining of IL-17A+ and CD3+ cells shows that IL-17A (in green) and CD3 (in red) were detected in acne vulgaris lesions. Cell nuclei were counterstained in blue. Arrows indicate IL-17A and CD3 double positive cells (b). T-bet+ cells (c) were more numerous than IL-17A+ cells or Foxp3+ cells (d) in the lesional acne skin. Large number of CD4+ cells (e), mostly lymphocytes, was seen around pilosebaceous unit and perivascularly. CD8+ cells (f) were fewer in number than CD4+ cells. Large number of CD68+ macrophages (g) and a few CD83+ cells (h), which are mature dendritic cells, were detected around sebaceous follicles. (Bar = 200 µm; in the insets bar = 50 µm). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25153527), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF317

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF-317-SP

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MAB317

IL-17/IL-17A Antibody (41809) [Unconjugated]

(28 Publications)

100 μg

MAB317

IL‑17/IL‑17A was detected in immersion fixed paraffin-embedded sections of human Crohn's disease intestine using Mouse Anti-Human/Primate IL‑17/IL‑17A Monoclonal Antibody (Catalog # MAB317) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Human IL-17 was immunoprecipitated from 100 µg of human primary differentiated Th17 cell lysate following incubation with 3 µg Mouse Anti-Human/Primate IL-17 Monoclonal Antibody (Catalog # MAB317) or isotype control antibody (Catalog # MAB004) overnight at 4 °C. IL-17-antibody complexes were absorbed using anti-mouse agarose beads. Immunoprecipitated IL-17 was detected by Western blot using 1 µg/mL Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA). View our recommended buffer recipes for immunoprecipitation.

Recombinant Human IL‑17 (Catalog # 317-ILB) stimulates IL‑6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line), as measured by the Mouse IL‑6 Quantikine ELISA Kit (Catalog # M6000B). IL‑6 secretion elicited by Recombinant Human IL‑17 (15 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate IL‑17 Monoclonal Antibody (Catalog # MAB317). The ND50 is typically 1-3 µg/mL.

Formalin-fixed paraffin-embedded tissue sections of human Crohn’s Disease Intestine were probed for IL-17A mRNA (ACD RNAScope Probe, catalog #310931; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using mouse anti-human IL-17A monoclonal antibody (R&D Systems catalog # MAB317) at 5ug/mL with 1 hour incubation at room temperature followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes.

Reactivity:

Hu, Pm

Species Glossary

Reactivity: Hu, Pm

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB317U
Alexa Fluor 405	FAB317V
Alexa Fluor 488	IC317G-100UG
Alexa Fluor 532	FAB317X
Alexa Fluor 594	FAB317T
Alexa Fluor 647	FAB317R
Alexa Fluor 700	FAB317N
Alexa Fluor 750	FAB317S
Allophycocyanin	IC317A
Biotin	FAB317B
CoraFluor 1	FAB317CL1
DyLight 350	FAB317D
DyLight 405	FAB317E
DyLight 488	FAB317K
DyLight 550	FAB317L
DyLight 594	FAB317M
DyLight 650	FAB317W
DyLight 680	FAB317Y
DyLight 755	FAB317Z
FITC	FAB317F
HRP	FAB317H
Janelia Fluor 525	FAB317JF525
Janelia Fluor 549	FAB317I
Janelia Fluor 585	FAB317JF585
Janelia Fluor 635	FAB317JF635
Janelia Fluor 646	FAB317J
Janelia Fluor 669	FAB317JF669
PerCP	FAB317C
Phycoerythrin	IC317P
mFluor Violet 450 SE	FAB317MFV450
mFluor Violet 500 SE	FAB317MFV500
mFluor Violet 610 SE	FAB317MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC317G-100UG
Carrier Free	IC317P
Carrier Free	IC317A
Carrier Free	MAB317-SP
Carrier Free	MAB317-500

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MAB721

IL-17/IL-17A Antibody (50101) [Unconjugated]

(25 Publications)

100 μg

MAB721

Reactivity:

Species Glossary

Reactivity: Mu

Applications:

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB721-SP
Carrier Free	MAB721-500

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AF-421-NA

IL-17/IL-17A Antibody [Unconjugated]

(18 Publications)

100 μg

AF-421-NA

EL-4 mouse lymphoblast cell line was treated for 16 hours with 50 ng/mL PMA then stained with Goat Anti-Mouse IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-421-NA, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reactivity:

Species Glossary

Reactivity: Mu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF421
PerCP (Peridinin-chlorophyll Protein Complex)	IC421C

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC421C
Carrier Free	AF-421-SP

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MAB3171

IL-17/IL-17A Antibody (41802) [Unconjugated]

(10 Publications)

100 μg

MAB3171

Western blot shows lysates of purified naïve human CD4+T cells orin vitrodifferentiated human Th17 CD4+ T cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IL-17 Monoclonal Antibody (Catalog # MAB3171) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-17 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IL‑17/IL‑17A was detected in immersion fixed paraffin-embedded sections of human Crohn's disease intestine using Mouse Anti-Human IL‑17/IL‑17A Monoclonal Antibody (Catalog # MAB3171) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Human IL-17 was immunoprecipitated from 100 µg of humanin vitrodifferentiated Th17 cell lysate following incubation with 3 µg Mouse Anti-Human IL-17 Monoclonal Antibody (Catalog # MAB3171) or isotype control antibody (Catalog # MAB002) overnight at 4 °C. IL-17-antibody complexes were absorbed using anti-mouse agarose beads. Immunoprecipitated IL-17 was detected by Western blot using 1 µg/mL Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA). View our recommended buffer recipes for immunoprecipitation.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC3171U-100UG
Alexa Fluor 405	IC3171V-100UG
Alexa Fluor 488	IC3171G-100UG
Alexa Fluor 532	FAB3171X
Alexa Fluor 594	IC3171T-100UG
Alexa Fluor 647	IC3171R-100UG
Alexa Fluor 700	IC3171N-100UG
Alexa Fluor 750	IC3171S-100UG
Allophycocyanin	IC3171A-100
Allophycocyanin	IC3171A-025
Biotin	FAB3171B
CoraFluor 1	FAB3171CL1
DyLight 350	FAB3171D
DyLight 405	FAB3171E
DyLight 488	FAB3171K
DyLight 550	FAB3171L
DyLight 594	FAB3171M
DyLight 650	FAB3171W
DyLight 680	FAB3171Y
DyLight 755	FAB3171Z
FITC	FAB3171F
HRP	FAB3171H
Janelia Fluor 525	FAB3171JF525
Janelia Fluor 549	FAB3171I
Janelia Fluor 585	FAB3171JF585
Janelia Fluor 635	FAB3171JF635
Janelia Fluor 646	FAB3171J
Janelia Fluor 669	FAB3171JF669
PerCP (Peridinin-chlorophyll Protein Complex)	IC3171C-025
PerCP (Peridinin-chlorophyll Protein Complex)	IC3171C-100
Phycoerythrin	IC3171P-025
Phycoerythrin	IC3171P-100
mFluor Violet 450 SE	FAB3171MFV450
mFluor Violet 500 SE	FAB3171MFV500
mFluor Violet 610 SE	FAB3171MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC3171G-100UG
Carrier Free	IC3171C-100
Carrier Free	IC3171P-100
Carrier Free	IC3171A-100
Carrier Free	IC3171A-025
Carrier Free	IC3171C-025
Carrier Free	IC3171P-025
Carrier Free	MAB3171-SP
Carrier Free	MAB3171-500
Carrier Free	IC3171S-100UG
Carrier Free	IC3171R-100UG
Carrier Free	IC3171N-100UG
Carrier Free	IC3171V-100UG
Carrier Free	IC3171U-100UG
Carrier Free	IC3171T-100UG

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NBP1-76337

IL-17/IL-17A Antibody - BSA Free

(11 Publications)

0.1 mg

NBP1-76337

Western Blot: IL-17/IL-17A Antibody [NBP1-76337] - Loading: 10 ug of lysates per lane. Antibodies: IL-17 4887, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Exposure: 1 min Lane 1: 1 ug/mL Lane 2: 2 ug/mL

Immunocytochemistry/Immunofluorescence: IL-17/IL-17A Antibody [NBP1-76337] - Immunofluorescence of IL-17 in A-20 cells with IL-17 antibody at 5 ug/ml.

Immunocytochemistry/Immunofluorescence: IL-17/IL-17A Antibody [NBP1-76337] - Mouse thymus tissue with IL-17 Antibody at 20 ug/mL.

Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody [NBP1-76337] - IL-17 in human tonsil tissue with IL-17 antibody at 5 ug/ml.

Reactivity:

Hu, Mu, RM

Species Glossary

Reactivity: Hu, Mu, RM

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-76337AF350
Alexa Fluor 405	NBP1-76337AF405
Alexa Fluor 488	NBP1-76337AF488
Alexa Fluor 532	NBP1-76337AF532
Alexa Fluor 594	NBP1-76337AF594
Alexa Fluor 647	NBP1-76337AF647
Alexa Fluor 700	NBP1-76337AF700
Alexa Fluor 750	NBP1-76337AF750
Biotin	NBP1-76337B
CoraFluor 1	NBP1-76337CL1
DyLight 350	NBP1-76337UV
DyLight 405	NBP1-76337V
DyLight 488	NBP1-76337G
DyLight 550	NBP1-76337R
DyLight 594	NBP1-76337DL594
DyLight 650	NBP1-76337C
DyLight 680	NBP1-76337FR
DyLight 755	NBP1-76337IR
FITC	NBP1-76337F
HRP	NBP1-76337H
Janelia Fluor 525	NBP1-76337JF525
Janelia Fluor 549	NBP1-76337JF549
Janelia Fluor 585	NBP1-76337JF585
Janelia Fluor 635	NBP1-76337JF635
Janelia Fluor 646	NBP1-76337JF646
Janelia Fluor 669	NBP1-76337JF669
PerCP	NBP1-76337PCP
mFluor Violet 450 SE	NBP1-76337MFV450
mFluor Violet 500 SE	NBP1-76337MFV500
mFluor Violet 610 SE	NBP1-76337MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-76337-0.025mg

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NBP1-42772

IL-17/IL-17A Antibody - Azide and BSA Free

(4 Publications)

0.1 mg

NBP1-42772

Western Blot: IL-17/IL-17A Antibody [NBP1-42772] - Analysis using the HRP conjugate of NBP1-42772. Detection of Lane 1: Recombinant Rat IL-17A. Load: 50 ng per lane. Used at a dilution of 1:1,000 o/n at 4C.

Western Blot: IL-17/IL-17A Antibody [NBP1-42772] - Analysis of mouse recombinant IL-17A protein (arrowhead, lane 1). Approximately 2 ug of recombinant protein was loaded onto the gel. Primary antibody was used at a 1:1,000 dilution. The membrane was washed and reacted with a 1:20,000 dilution of DyLight 649 conjugated Gt-a-Rabbit IgG.

Western blot using anti-IL-17A antibody shows detection of rat recombinant IL-17A protein (lane 1). Approximately 2 ug of recombinant protein was loaded onto the gel. Primary antibody was used at a 1:1,000 dilution. The membrane was washed and reacted with a 1:20,000 dilution of DyLight(TM) 649 conjugated Gt-a-Rabbit IgG

Western blot using anti-IL-17A antibody shows detection of rat recombinant IL-17A protein (arrowhead, lane 1). Approximately 2 ug of recombinant protein was loaded onto the gel. Primary antibody was used at a 1:1,000 dilution. The membrane was washed and reacted with a 1:20,000 dilution of DyLight(TM) 649 conjugated Gt-a-Rabbit IgG

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-27338

IL-17/IL-17A Antibody (4H1524)

(2 Publications)

0.1 mg

NBP2-27338

Block/Neutralize: IL-17/IL-17A Antibody (4H1524) [NBP2-27338] - Analysis using Azide Free version of NBP2-27338. Neutralization by Human IL-17A Antibody. Secretion of IL-6 by 3T3 cells stimulated with recombinant human IL-17A (20ng/ml) is neutralized by increasing concentrations of IL-17A monoclonal antibody. Antibody

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-6580A

Alt Catalog# IMG-6580A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
PE	NBP2-27252

Available Formulations

Formulation	Catalog #	Availability	Price
Azide Free	NBP2-25229

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NBP3-13294

IL-17/IL-17A Antibody

(1 Publication)

100 ul

NBP3-13294

Western Blot: IL-17/IL-17A Antibody [NBP3-13294] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with IL-17/IL-17A antibody (NBP3-13294) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: IL-17/IL-17A Antibody [NBP3-13294] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with IL-17/IL-17A antibody (NBP3-13294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody [NBP3-13294] - Human colon cancer. IL-17/IL-17A stained by IL-17/IL-17A antibody (NBP3-13294) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Reactivity:

Hu, Mu, Rt, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Ca

Applications:

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-72027

IL-17/IL-17A Antibody (TC11-18H10) - Azide and BSA Free

(3 Publications)

0.1 mg

NBP1-72027

Immunocytochemistry/ Immunofluorescence: IL-17/IL-17A Antibody (TC11-18H10) - Azide and BSA Free [NBP1-72027] - TG2-dependent chemokine production in keratinocytes is critical for IL-17-producing CCR6+ gamma δT-cell & neutrophil dermal infiltration.a, b Effect of DMSO- or IMQ-treated WT- or TG2−/−-CM on CCR6+ gamma δT-cell (a) & CD11b+ Ly-6G+ cell (b) migration according to Transwell migration assays. Flow cytometric analysis of the percentage of cells migrating toward the CM in the presence or absence of anti-CXCL1 (a) & anti-CCL20 (b) antibodies (n = 3/group). c, d WT & TG2−/− mouse-derived CCR6+ gamma δT-cell (c) & CD11b+ Ly-6G+ cell (d) migration in response to recombinant mouse CCL20 & CXCL1, respectively. Data represent the mean ± SEM (n = 3/group). *p < 0.05; **p < 0.01. e, f Cells were isolated from the back skin of WT & TG2−/− mice treated with IMQ for four consecutive days & analyzed by flow cytometry. Representative flow cytometric profiles of CCR6+ gamma δTCRlow cells (e) & CD11bhigh Ly-6G+ cells (f). The percentage of cells is shown. Data represent the mean ± SEM (n = 9/group). g Skin sections were immunostained with CCR6 (green)- & IL-17 (red)-specific antibodies. Nuclei were stained with DAPI (blue). Scale bar, 100 μm. h–j Back skin was separated into epidermis & dermis & analyzed by RT-PCR for IL-17-producing gamma δT-cell markers (h) (Ccr6 & Il23r), IL17 & related cytokines (i) (Il17a, Il17f, & Il22), & myeloid cell-derived cytokines (j) (Il12a & Il23a). Data represent the mean ± SEM (n = 11/group). *p < 0.05; **p < 0.01 vs. WT mice. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32355189), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Species Glossary

Reactivity: Mu

Applications:

, , ,

Applications: , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-72027AF350
Alexa Fluor 405	NBP1-72027AF405
Alexa Fluor 488	NBP1-72027AF488
Alexa Fluor 532	NBP1-72027AF532
Alexa Fluor 594	NBP1-72027AF594
Alexa Fluor 647	NBP1-72027AF647
Alexa Fluor 700	NBP1-72027AF700
Alexa Fluor 750	NBP1-72027AF750
Allophycocyanin	NBP1-72027APC
Allophycocyanin/Cy7	NBP1-72027APCCY7
Biotin	NBP1-72027B
CoraFluor 1	NBP1-72027CL1
DyLight 350	NBP1-72027UV
DyLight 405	NBP1-72027V
DyLight 488	NBP1-72027G
DyLight 550	NBP1-72027R
DyLight 594	NBP1-72027DL594
DyLight 650	NBP1-72027C
DyLight 680	NBP1-72027FR
DyLight 755	NBP1-72027IR
FITC	NBP1-72027F
HRP	NBP1-72027H
Janelia Fluor 525	NBP1-72027JF525
Janelia Fluor 549	NBP1-72027JF549
Janelia Fluor 585	NBP1-72027JF585
Janelia Fluor 635	NBP1-72027JF635
Janelia Fluor 646	NBP1-72027JF646
Janelia Fluor 669	NBP1-72027JF669
PE	NBP1-72027PE
PE/Atto594	NBP1-72027PEATT594
PE/Cy5.5	NBP1-72027PECY55
PE/Cy7	NBP1-72027PECY7
PerCP	NBP1-72027PCP
mFluor Violet 450 SE	NBP1-72027MFV450
mFluor Violet 500 SE	NBP1-72027MFV500
mFluor Violet 610 SE	NBP1-72027MFV610

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NBP2-24765

IL-17/IL-17A Antibody (4k5F6) - BSA Free

0.1 mg

NBP2-24765

Reactivity:

Hu, Mu, Rt, Bv, Eq, Pm, Ma-Op

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Eq, Pm, Ma-Op

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-6545A

Alt Catalog# IMG-6545A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-24765AF350
Alexa Fluor 405	NBP2-24765AF405
Alexa Fluor 488	NBP2-24765AF488
Alexa Fluor 532	NBP2-24765AF532
Alexa Fluor 594	NBP2-24765AF594
Alexa Fluor 647	NBP2-24765AF647
Alexa Fluor 700	NBP2-24765AF700
Alexa Fluor 750	NBP2-24765AF750
Biotin	NBP2-24765B
CoraFluor 1	NBP2-24765CL1
DyLight 350	NBP2-24765UV
DyLight 405	NBP2-24765V
DyLight 488	NBP2-24765G
DyLight 550	NBP2-24765R
DyLight 594	NBP2-24765DL594
DyLight 650	NBP2-24765C
DyLight 680	NBP2-24765FR
DyLight 755	NBP2-24765IR
FITC	NBP2-24765F
HRP	NBP2-24765H
Janelia Fluor 525	NBP2-24765JF525
Janelia Fluor 549	NBP2-24765JF549
Janelia Fluor 585	NBP2-24765JF585
Janelia Fluor 635	NBP2-24765JF635
Janelia Fluor 646	NBP2-24765JF646
Janelia Fluor 669	NBP2-24765JF669
mFluor Violet 450 SE	NBP2-24765MFV450
mFluor Violet 500 SE	NBP2-24765MFV500
mFluor Violet 610 SE	NBP2-24765MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-24765SS
Azide and BSA Free	NBP2-46611

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

IL-17/IL-17A Antibodies

IL-17/IL-17A Antibody (50104) [Unconjugated]

IL-17/IL-17A Antibody [Unconjugated]

IL-17/IL-17A Antibody (41809) [Unconjugated]

IL-17/IL-17A Antibody (50101) [Unconjugated]

IL-17/IL-17A Antibody [Unconjugated]

IL-17/IL-17A Antibody (41802) [Unconjugated]

IL-17/IL-17A Antibody - BSA Free

IL-17/IL-17A Antibody - Azide and BSA Free

IL-17/IL-17A Antibody (4H1524)

IL-17/IL-17A Antibody

IL-17/IL-17A Antibody (TC11-18H10) - Azide and BSA Free

IL-17/IL-17A Antibody (4k5F6) - BSA Free