Home » IL-17/IL-17A » IL-17/IL-17A Antibodies » IL-17/IL-17A Antibody (41802) [Alexa Fluor® 488]
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IL-17/IL-17A Antibody (41802) [Alexa Fluor® 488]

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Human peripheral blood mononuclear cells (PBMCs) treated with PMA, Calcium Ionomycin, LPS, and Recombinant Human IL-23 (Catalog # 1290-IL) to induce Th17 development overnight, re-stimulated with PMA, Calcium Ionomycin, and Monensin for 2 hours were stained with Mouse Anti-Human IL-17/IL-17A Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC3171G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). Histograms were gated on CD4+cells. View our protocol for Staining Intracellular Molecules.
Human peripheral blood mononuclear cells (PBMCs) treated with PMA, Calcium Ionomycin, LPS, and Recombinant Human IL-23 (Catalog # 1290-IL) to induce Th17 development overnight, re-stimulated with PMA, Calcium Ionomycin, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
41802
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 488

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC3171U-100UG
Alexa Fluor 405
IC3171V-100UG
Alexa Fluor 532
FAB3171X
Alexa Fluor 594
IC3171T-100UG
Alexa Fluor 647
IC3171R-100UG
Alexa Fluor 700
IC3171N-100UG
Alexa Fluor 750
IC3171S-100UG
Allophycocyanin
IC3171A-100
Allophycocyanin
IC3171A-025
Biotin
FAB3171B
CoraFluor 1
FAB3171CL1
DyLight 350
FAB3171D
DyLight 405
FAB3171E
DyLight 488
FAB3171K
DyLight 550
FAB3171L
DyLight 594
FAB3171M
DyLight 650
FAB3171W
DyLight 680
FAB3171Y
DyLight 755
FAB3171Z
FITC
FAB3171F
HRP
FAB3171H
Janelia Fluor 525
FAB3171JF525
Janelia Fluor 549
FAB3171I
Janelia Fluor 646
FAB3171J
Janelia Fluor 669
FAB3171JF669
PerCP (Peridinin-chlorophyll Protein Complex)
IC3171C-025
PerCP (Peridinin-chlorophyll Protein Complex)
IC3171C-100
Phycoerythrin
IC3171P-025
Phycoerythrin
IC3171P-100
Unconjugated
MAB3171-100
mFluor Violet 450 SE
FAB3171MFV450
mFluor Violet 500 SE
FAB3171MFV500
mFluor Violet 610 SE
FAB3171MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB3171-100
Carrier Free
MAB3171-100
Carrier Free
IC3171U-100UG
Carrier Free
IC3171V-100UG
Carrier Free
IC3171N-100UG
Carrier Free
IC3171R-100UG
Carrier Free
IC3171S-100UG
Carrier Free
MAB3171-500
Carrier Free
MAB3171-SP
Carrier Free
IC3171P-025
Carrier Free
IC3171C-025
Carrier Free
IC3171A-025
Carrier Free
IC3171A-100
Carrier Free
IC3171P-100
Carrier Free
IC3171C-100
Carrier Free
IC3171T-100UG
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IL-17/IL-17A Antibody (41802) [Alexa Fluor® 488] Summary

Immunogen
E. coli-derived recombinant human IL-17/IL-17A
Ile20-Ala155
Accession # Q16552
Specificity
Detects human IL-17/IL-17A in direct ELISAs. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) IL-17A/IL-17F heterodimer is observed. No cross-reactivity with recombinant mouse IL-17/IL-17A, recombinant canine IL-17/IL-17A, rhIL-17B, rhIL-17C, rhIL-17D, rhIL-17E, or rhIL-17F is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL17A
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 5 uL/10^6 cells

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17/IL-17A Antibody (41802) [Alexa Fluor® 488]

  • CTLA8
  • CTLA-8
  • CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
  • Cytotoxic T-lymphocyte-associated antigen 8
  • IL17
  • IL-17
  • IL17A
  • IL-17A
  • IL-17Acytotoxic T-lymphocyte-associated protein 8
  • IL-17CTLA-8
  • IL17interleukin-17A
  • interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
  • interleukin 17A

Background

Interleukin-17 (IL-17), also known as IL-17A, is a pro-inflammatory cytokine secreted by activated T cells. It is the prototype member of the IL-17 family that also includes IL-17B, C, D, E, and F.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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IL-17/IL-17A
Antibody (41802) [Ale ...
IC3171G
IL-17/IL-17A Antibody (41802) [Alexa Fluor® 488]
Species: Hu
Applications: Flow

Publications for IL-17/IL-17A Antibody (IC3171G) (0)

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FAQs for IL-17/IL-17A Antibody (IC3171G). (Showing 1 - 2 of 2 FAQ).

  1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
    • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
  2. I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
    • Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol IL17A
Uniprot