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IL-17/IL-17A Antibody [Unconjugated]

Images

 
Western blot shows lysates of human primary naïve CD4+ T cells, along with whole cell lysates (WCL) and conditioned-media supernatant (Supe) of human primary differentiated Th17 cells. PVDF membrane was probed with 1 ...read more
IL‑17/IL‑17A was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL‑17/IL‑17A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA) at 1 ...read more
Human IL-17 was immunoprecipitated from 100 µg of human primary differentiated Th17 cell lysate following incubation with 3 µg Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA) ...read more
Human peripheral blood mononuclear cells were unstimulated (light orange filled histogram) or treated with 50 ng/mL PMA and 250 ng/mL Ca2+ ionomycin for 16 hours, then stained with Goat Anti-Human IL‑17 Antigen ...read more
Recombinant Human IL‑17 (Catalog # 317-ILB) stimulates IL‑6 secretion in NIH/3T3 mouse embryonic fibroblasts in a dose-dependent manner (orange line), as measured by the mouse IL‑6 Quantikine ELISA Kit (Catalog # ...read more
Expression of psoriasis‐related cytokines in paradoxical psoriasiform reactions. Immunohistochemistry analysis performed on paradoxical skin lesions obtained from patients 1 (Pt1) and 2 (Pt2) shows similar values of ...read more
Inflammatory cell infiltrates of early acne lesions in immunohistochemistry.Representative immunohistochemical staining showing IL-17A+ cells in early acne lesion (a). Staining of IL-17A+ and CD3+ cells shows that ...read more
Inflammatory cell infiltrates of early acne lesions in immunohistochemistry.Representative immunohistochemical staining showing IL-17A+ cells in early acne lesion (a). Staining of IL-17A+ and CD3+ cells shows that ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP, B/N, CyTOF-ready, Flow, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price
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IL-17/IL-17A Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IL-17
Ile20-Ala155
Accession # Q16552
Specificity
Detects human IL-17 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant canine IL-17 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL17A
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Immunoprecipitation 3 ug/100 ug cell lysate
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 0.02-0.12 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 4 Reviews rated 4.3
using
AF-317-NA in the following applications:

Publications
Read Publications using
AF-317-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17/IL-17A Antibody [Unconjugated]

  • CTLA8
  • CTLA-8
  • CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
  • Cytotoxic T-lymphocyte-associated antigen 8
  • IL17
  • IL-17
  • IL17A
  • IL-17A
  • IL-17Acytotoxic T-lymphocyte-associated protein 8
  • IL-17CTLA-8
  • IL17interleukin-17A
  • interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
  • interleukin 17A

Background

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Human IL-17 cDNA encodes a 155 amino acid (aa) residue precursor protein with a 19 amino acid residue signal peptide that is cleaved to yield the 136 aa residue mature IL-17 containing one potential N-linked glycosylation site. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, human IL-17 shows 72% and 63% sequence identity with herpesvirus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has recently been cloned. While the expression of IL-17 mRNA is restricted to activated T cells, the expression of mIL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including the induction of IL-6 and IL-8 production in fibroblasts, the enhancement of surface expression of ICAM-1 in fibroblasts, activation of NF-kappa B and costimulation of T cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-17/IL-17A Antibody (AF-317-NA)(95)

We have publications tested in 3 confirmed species: Human, Mouse, Canine.

We have publications tested in 12 applications: Cell Culture, ELISA Development, Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Immunocytochemistry, Immunohistochemistry, Neutralization, Tissue Culture, Western Blot.


Filter By Application
Cell Culture
(1)
ELISA Development
(1)
Flow Cytometry
(1)
ICC
(1)
IHC
(21)
IHC-Fr
(2)
IHC-P
(21)
Immunocytochemistry
(1)
Immunohistochemistry
(1)
Neutralization
(9)
Tissue Culture
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(52)
Mouse
(1)
Canine
(1)
All Species
Showing Publications 1 - 10 of 95. Show All 95 Publications.
Publications using AF-317-NA Applications Species
Tsukalov, I;Sánchez-Cerrillo, I;Rajas, O;Avalos, E;Iturricastillo, G;Esparcia, L;Buzón, MJ;Genescà, M;Scagnetti, C;Popova, O;Martin-Cófreces, N;Calvet-Mirabent, M;Marcos-Jimenez, A;Martínez-Fleta, P;Delgado-Arévalo, C;de Los Santos, I;Muñoz-Calleja, C;Calzada, MJ;González Álvaro, I;Palacios-Calvo, J;Alfranca, A;Ancochea, J;Sánchez-Madrid, F;Martin-Gayo, E; NF?B and NLRP3/NLRC4 inflammasomes regulate differentiation, activation and functional properties of monocytes in response to distinct SARS-CoV-2 proteins Nature communications 2024-03-07 [PMID: 38453949] (Immunohistochemistry, Immunocytochemistry, Human) Immunohistochemistry, Immunocytochemistry Human
Mick J.M. van Eijs, José J.M. Ter Linde, Matthijs J.D. Baars, Mojtaba Amini, Miangela M. Laclé, Eelco C. Brand et al. Highly multiplexed spatial analysis identifies tissue-resident memory T cells as drivers of ulcerative and immune checkpoint inhibitor colitis iScience 2023-10-20 [PMID: 37766980]
Maria Luigia Carbone, Alessia Capone, Marika Guercio, Sofia Reddel, Domenico Alessandro Silvestris, Daniela Lulli et al. Insight into immune profile associated with vitiligo onset and anti-tumoral response in melanoma patients receiving anti-PD-1 immunotherapy Frontiers in Immunology 2023-08-23 [PMID: 37680638]
Oyler BL, Valencia-Dávila JA, Moysi E et al. Multilevel human secondary lymphoid immune system compartmentalization revealed by complementary imaging approaches iScience 2023-08-18 [PMID: 37520703]
Xue Liu, Chenyang Xu, Tianwei Guo, Shenghua Zhan, Qiuying Quan, Mengsi Li et al. Clinical significance of CD155 expression and correlation with cellular components of tumor microenvironment in gastric adenocarcinoma Frontiers in Immunology 2023-06-27 [PMID: 37441080]
Jennifer S. Chen, Michael J. Murphy, Katelyn Singh, Alice Wang, Ryan D. Chow, Sa Rang Kim et al. IL17A mRNA Staining Distinguishes Palmoplantar Psoriasis from Hyperkeratotic Palmoplantar Eczema in Diagnostic Skin Biopsies JID Innovations 2023-07-01 [PMID: 37205304]
Simon Bossart, S. Morteza Seyed Jafari, Kristine Heidemeyer, Kexiang Yan, Laurence Feldmeyer, Luca Borradori et al. Canakinumab leads to rapid reduction of neutrophilic inflammation and long-lasting response in Schnitzler syndrome Frontiers in Medicine 2023-03-15 [PMID: 37007766]
A Schäbitz, C Hillig, M Mubarak, M Jargosch, A Farnoud, E Scala, N Kurzen, AC Pilz, N Bhalla, J Thomas, M Stahle, T Biedermann, CB Schmidt-We, F Theis, N Garzorz-St, K Eyerich, MP Menden, S Eyerich Spatial transcriptomics landscape of lesions from non-communicable inflammatory skin diseases Nature Communications, 2022-12-13;13(1):7729. 2022-12-13 [PMID: 36513651] (IHC, Human) IHC Human
Zheng N, Fang J, Xue G et al. Induction of tumor cell autosis by myxoma virus-infected CAR-T and TCR-T cells to overcome primary and acquired resistance Cancer cell 8/15/2022 [PMID: 36027915]
Bronte Manouk Verhoeven, Shenglin Mei, Thale Kristin Olsen, Karin Gustafsson, Anders Valind, Axel Lindström et al. The immune cell atlas of human neuroblastoma Cell Reports Medicine 2022-06-09 [PMID: 35688160]
Show All 95 Publications.

Reviews for IL-17/IL-17A Antibody (AF-317-NA) (4) 4.34

Average Rating: 4.3
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Other.

Reviews using AF-317-NA:
Filter by Applications
ICC
(2)
IHC-P
(2)
All Applications
Filter by Species
Human
(3)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry IL-17/IL-17A AF-317-NA
Enlarge
5
reviewed by:
Manuel Flores
ICC Human 03/24/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedFFPE human liver,FFPE
SpeciesHuman

Comments

CommentsMultiplex immnunofluorescence labelling IL-17A , CD4 , and FoxP3 on FFPE section of human liver biopsy. IL-17A primary Ab is the AF-317-NA from Novus Biologicals. Secondary Ab is A568. FFPE sections were dewaxed with xylene and rehydrated with ethanol solutions (decreasing %). Antigen retrieval was performed with sodium citrate pH6 in a pressure cooker. IL-17A signal is specific, and the staining pattern is what you would expect for a molecule that is produced in the cytoplasm and secreted.
  4
reviewed by:
Verified Customer
IHC-P Human 01/07/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 20426778
SpeciesHuman
  4
reviewed by:
Verified Customer
IHC-P Other 01/07/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 20357258
SpeciesOther
  4
reviewed by:
Verified Customer
ICC Human 01/07/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 21307293
SpeciesHuman

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FAQs for IL-17/IL-17A Antibody (AF-317-NA). (Showing 1 - 2 of 2 FAQs).

  1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
    • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
  2. I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
    • Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).
mFluor Violet Conjugated Antibodies

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Recent Reviews

4.3
5
1
4
3
3
0
2
0
1
0

Manuel Flores
03/24/2017
Application: ICC
Species: Human

 
Verified Customer
01/07/2015
Application: IHC-P
Species: Human

 
Verified Customer
01/07/2015
Application: IHC-P
Species: Other

Bioinformatics

Gene Symbol IL17A
Uniprot