IL-17/IL-17A Antibody (4H1524) - Azide Free Summary
| Immunogen |
A portion of amino acids 1-100 was used as immunogen. |
| Isotype |
IgG2b Kappa |
| Clonality |
Monoclonal |
| Host |
Mouse |
| Gene |
IL17A |
| Purity |
Protein G purified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
- Block/Neutralize
- CyTOF-ready
- Flow Cytometry 1ul/1 million cells
- Western Blot 1:100-1:2000
|
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer |
0.2 ml sterile PBS |
| Preservative |
No Preservative |
| Purity |
Protein G purified |
Alternate Names for IL-17/IL-17A Antibody (4H1524) - Azide Free
Background
Interleukin 17 (IL-17) is a pro-inflammatory cytokine produced by a subset of T helper cells that develops distinct from the Th1- and Th2- cell differentiation pathways. IL-17, also known as CTLA-8, stimulates induction of other pro-inflammatory cytokines TNF alpha, IL-1 beta, IL-6, and IL-8, and reports strongly suggest the involvement of IL-17 in several chronic inflammatory diseases such as rheumatoid arthritis, psoriasis and multiple sclerosis. TGF-Beta (differentiation) and IL-23 (expansion) are required for induction and maintenance of Th17 (IL-17 producing) cells, which in turn induce the other pro-inflammatory cytokines. IL-17 (~32kDa) protein is produced and exists as a homo-dimer, has homology to a herpes virus early protein, is one of the six members (IL-17A-F) of this cytokine family, and is well characterzed and highly expressed by activated effector memory T cells.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
Customers Who Viewed This Item Also Viewed...
Species: Mu
Applications: ELISA
Species: Hu
Applications: BA
Species: Mu
Applications: ELISA
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
Species: Hu
Applications: BA
Species: Hu
Applications: BA
Species: Hu
Applications: BA
Species: Hu
Applications: BA
Species: Hu
Applications: CyTOF-ready, IHC, ICFlow, WB
Species: Bv, Hu, Mu, Rt
Applications: Dual ISH-IHC, Flow, IB, ICC/IF, IHC, IHC-P, IP, Single-Cell Western, WB
Species: Hu
Applications: BA
Species: Hu
Applications: ELISA
Species: Hu
Applications: ELISA
Species: Mu
Applications: ELISA
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, Neut, WB
Species: Mu
Applications: ELISA
Species: Hu
Applications: ELISA
Species: Hu
Applications: CyTOF-ready, Flow, KO, Neut, WB
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
Species: Hu
Applications: WB, Flow, B/N, CyTOF-ready
Publications for IL-17/IL-17A Antibody (NBP2-25229) (0)
There are no publications for IL-17/IL-17A Antibody (NBP2-25229).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for IL-17/IL-17A Antibody (NBP2-25229) (0)
There are no reviews for IL-17/IL-17A Antibody (NBP2-25229).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for IL-17/IL-17A Antibody (NBP2-25229). (Showing 1 - 2 of 2 FAQ).
-
I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
- Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
-
I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
- Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).
Secondary Antibodies
| |
Isotype Controls
|
Additional IL-17/IL-17A Products
Research Areas for IL-17/IL-17A Antibody (NBP2-25229)
Find related products by research area.
|
Blogs on IL-17/IL-17A
![Western Blot: IL-17/IL-17A Antibody (4H1524) - Azide Free [NBP2-25229] - Analysis of IL-17A in (1) human, (2) mouse and (3) rat full-length recombinant IL-17A protein using NBP2-25229 at 1 ug/ml.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-4H1524-Azide-Free-Western-Blot-NBP2-25229-img0001.jpg "Western Blot: IL-17/IL-17A Antibody (4H1524) - Azide Free [NBP2-25229] - Analysis of IL-17A in (1) human, (2) mouse and (3) rat full-length recombinant IL-17A protein using NBP2-25229 at 1 ug/ml.")
![Immunohistochemistry IL-17D Antibody (246018) [Unconjugated]](https://images.novusbio.com/images2/IL-17D_MAB15041_Immunohistochemistry_6949.jpg)
![Intracellular Staining by Flow Cytometry IL-17D Antibody (246018) [Unconjugated]](https://images.novusbio.com/images2/IL-17D_MAB15041_Flow_Cytometry_8338.jpg)
![Flow Cytometry: IL-17/IL-17A Antibody (4H1524) - Azide Free [NBP2-25229] - Analysis using the PE conjugate of NBP2-25229. IL-17A positive cells in PMA/IM stimulated fresh PBMC: PBMC stimulated for 6h with PMA (25ng/ml) & IM (250ng/ml), in the presence of BFA. Cells were surface stained with CD4 APC, fixed & permeabilizedfor intracellular staining with PE conjugated](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-4H1524-Azide-Free-Flow-Cytometry-NBP2-25229-img0004.jpg "Flow Cytometry: IL-17/IL-17A Antibody (4H1524) - Azide Free [NBP2-25229] - Analysis using the PE conjugate of NBP2-25229. IL-17A positive cells in PMA/IM stimulated fresh PBMC: PBMC stimulated for 6h with PMA (25ng/ml) & IM (250ng/ml), in the presence of BFA. Cells were surface stained with CD4 APC, fixed & permeabilizedfor intracellular staining with PE conjugated")
![Block/Neutralize: IL-17/IL-17A Antibody (4H1524) - Azide Free [NBP2-25229] - Neutralization by Human IL-17A Antibody. Secretion of IL-6 by 3T3 cells stimulated with recombinant human IL-17A (20ng/ml) is neutralized by increasing concentrations of IL-17A monoclonal antibody. Antibody at different concentrations was added to cells 1hr prior to addition of cytokine. Supernatant collected from 60hr cultures was used for analysis to measure secreted IL-6 by ELISA.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-4H1524-Azide-Free-Blocking-Neutralizing-NBP2-25229-img0002.jpg "Block/Neutralize: IL-17/IL-17A Antibody (4H1524) - Azide Free [NBP2-25229] - Neutralization by Human IL-17A Antibody. Secretion of IL-6 by 3T3 cells stimulated with recombinant human IL-17A (20ng/ml) is neutralized by increasing concentrations of IL-17A monoclonal antibody. Antibody at different concentrations was added to cells 1hr prior to addition of cytokine. Supernatant collected from 60hr cultures was used for analysis to measure secreted IL-6 by ELISA.")
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![N/A IL-10 [Biotin]](https://images.novusbio.com/images2/DATA_IL10_DY417_ELISA_2014.jpg)
![SEC-MALS IL-23A/IL-23 P19 [Unconjugated]](https://images.novusbio.com/images/1290-il_recombinant-human-il-23-protein-sec-mals-1012202411154..jpg)
![SDS-PAGE IL-22 [Unconjugated]](https://images.novusbio.com/images2/IL-22_782-IL_CF_734.jpg)
![Bioactivity IL-22 [Unconjugated]](https://images.novusbio.com/images2/IL-22_782-IL_CF_743.jpg)
![Bioactivity IL-4 [Unconjugated]](https://images.novusbio.com/images/protein/6507-ilcf_recombinant-human-il-4-cho-expressed-protein-cf-bioactivity-272020133214.jpg)
![Bioactivity IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFNgamma_285IF_2848.jpg)
![SDS-PAGE IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFN-gamma_285-IF_230.jpg)
![SEC-MALS IFN-gamma [Unconjugated]](https://images.novusbio.com/images/285-if_recombinant-human-ifn-gamma-protein-sec-mals-1520239228..jpg)
![Bioactivity IL-2 [Unconjugated]](https://images.novusbio.com/images/202-il_recombinant-human-il-2-protein-bioactivity-174202314946.jpg)
![N/A IL-17F [Biotin]](https://images.novusbio.com/images2/DATA_IL17F_DY1335_ELISA_2300.jpg)
![N/A IL-17F [Biotin]](https://images.novusbio.com/images2/DATA_IL17F_DY1335_ELISA_1626.jpg)
![N/A IL-37/IL-1F7 [Biotin]](https://images.novusbio.com/images2/DATA_IL37_DY1975_ELISA_1742.jpg)
![N/A IL-13 [Biotin]](https://images.novusbio.com/images2/DATA_IL13_DY413_ELISA_2010.jpg)
![Immunocytochemistry IL-21 Antibody [Unconjugated]](https://images.novusbio.com/images2/IL-21_AF594_Immunocytochemistry_6525.jpg)
![Neutralization IL-21 Antibody [Unconjugated]](https://images.novusbio.com/images2/af594_mouse-il-21-affinity-purified-polyclonal-ab-neutralization-249202192915.jpg)
![N/A IL-5 [HRP]](https://images.novusbio.com/images2/IL-5_M5000_ELISA_237.jpg)
![N/A IL-5 [HRP]](https://images.novusbio.com/images2/DATA_IL5_M5000_ELISA_935.jpg)
![N/A CCL2/JE/MCP-1 [HRP]](https://images.novusbio.com/images2/CCL2_DCP00_ELISA_67.jpg)
![N/A CCL2/JE/MCP-1 [HRP]](https://images.novusbio.com/images2/DATA_CCL2_DCP00_ELISA_633.jpg)
![N/A CCL2/JE/MCP-1 [HRP]](https://images.novusbio.com/images2/DATA_CCL2_DCP00_ELISA_634.jpg)
![Neutralization IL-17RA/IL-17R Antibody (133617) [Unconjugated]](https://images.novusbio.com/images2/IL17_RA_MAB177_Block_Neutralize_15487.jpg)
![Flow Cytometry IL-17RA/IL-17R Antibody (133617) [Unconjugated]](https://images.novusbio.com/images2/IL17_RA_MAB177_CyTOFready_23136.jpg)
![Knockout Validated IL-17RA/IL-17R Antibody (133617) [Unconjugated]](https://images.novusbio.com/images2/IL17_RA_MAB177_Knockout_Validated_23137.jpg)
