Western Blot: IL-17/IL-17A Antibody [NBP1-76337] - Analysis of 1 ng of recombinant human IL-17 with IL-17 antibody at 1 ug/ml.
Western Blot: IL-17/IL-17A Antibody [NBP1-76337] - Loading: 10 ug of lysates per lane. Antibodies: IL-17 4887, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 ...read more
Immunohistochemistry: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Mouse Colon Tissue. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-IL-17/IL-17A ...read more
Immunocytochemistry/ Immunofluorescence: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Mouse Heart Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart issue ...read more
Immunohistochemistry: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Human Tonsil Tissue.Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL-17/IL-17A ...read more
Immunocytochemistry/ Immunofluorescence: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Mouse Thymus Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus tissue ...read more
Immunohistochemistry: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Human Lymph Node.Immunohistochemical analysis of paraffin-embedded human lymph node tissue using anti-IL-17/IL-17A ...read more
Immunocytochemistry/ Immunofluorescence: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Mouse A-20 Cells.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse A-20 Cells labeling ...read more
Antibody was raised against a peptide corresponding to 16 amino acids near the center of mature human IL-17. The immunogen is located within amino acids 50-100 of IL-17.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IL17A
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Immunogen displays the following percentage of sequence identity for non-tested species: Bovine: (84%), Rat: (81%). Use in Rhesus Macaque reported in scientific literature (PMID:32277724).
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified
Alternate Names for IL-17/IL-17A Antibody - BSA Free
Interleukin 17 (IL-17) is a family of pro-inflammatory cytokines produced by activated T cells and is thought to have a major role in the initiation and perpetuation of rheumatoid arthritis. IL-17 regulates the activities of NF-kappaB and mitogen-activated protein kinases such as ERK and JNK. In addition, IL-17 stimulates the expression of IL-6 and cyclooxygenase-2 and enhances the production of nitric oxide. IL-17-producing T helper cells (TH-17 cells) have been the subject of much attention due to the importance of IL-17 in the pathogenesis of autoimmune inflammation. Because of its role in autoimmune diseases, it is thought that targeting the production and action of IL-17 would be beneficial therapeutically in these diseases.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for IL-17/IL-17A Antibody (NBP1-76337). (Showing 1 - 2 of 2 FAQs).
I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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![Western Blot: IL-17/IL-17A Antibody [NBP1-76337] - Analysis of 1 ng of recombinant human IL-17 with IL-17 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP1-76337-img0013.jpg "Western Blot: IL-17/IL-17A Antibody [NBP1-76337] - Analysis of 1 ng of recombinant human IL-17 with IL-17 antibody at 1 ug/ml.")
![Immunohistochemistry IL-17D Antibody (246018) [Unconjugated]](https://images.novusbio.com/images2/IL-17D_MAB15041_Immunohistochemistry_6949.jpg)
![Intracellular Staining by Flow Cytometry IL-17D Antibody (246018) [Unconjugated]](https://images.novusbio.com/images2/IL-17D_MAB15041_Flow_Cytometry_8338.jpg)
![Western Blot: IL-17/IL-17A Antibody [NBP1-76337] - Loading: 10 ug of lysates per lane. Antibodies: IL-17 4887, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Exposure: 1 min Lane 1: 1 ug/mL Lane 2: 2 ug/mL](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP1-76337-img0012.jpg "Western Blot: IL-17/IL-17A Antibody [NBP1-76337] - Loading: 10 ug of lysates per lane. Antibodies: IL-17 4887, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Exposure: 1 min Lane 1: 1 ug/mL Lane 2: 2 ug/mL")
![Immunohistochemistry: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Mouse Colon Tissue. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-IL-17/IL-17A antibody at 2 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76337_rabbit-polyclonal-il-17-il-17a-antibody-2711202420414565.gif "Immunohistochemistry: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Mouse Colon Tissue. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-IL-17/IL-17A antibody at 2 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
![Immunocytochemistry/ Immunofluorescence: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Mouse Heart Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart issue labeling IL-17/IL-17A with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76337_rabbit-polyclonal-il-17-il-17a-antibody-271120241928814.jpg "Immunocytochemistry/ Immunofluorescence: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Mouse Heart Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart issue labeling IL-17/IL-17A with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).")
![Immunohistochemistry: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Human Tonsil Tissue.Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL-17/IL-17A antibody at 5 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76337_rabbit-polyclonal-il-17-il-17a-antibody-2711202420312943.gif "Immunohistochemistry: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Human Tonsil Tissue.Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL-17/IL-17A antibody at 5 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
![Immunocytochemistry/ Immunofluorescence: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Mouse Thymus Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus tissue labeling IL-17/IL-17A with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-76337_rabbit-polyclonal-il-17-il-17a-antibody-2711202419293859.gif "Immunocytochemistry/ Immunofluorescence: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Mouse Thymus Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus tissue labeling IL-17/IL-17A with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).")
![Immunohistochemistry: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Human Lymph Node.Immunohistochemical analysis of paraffin-embedded human lymph node tissue using anti-IL-17/IL-17A antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76337_rabbit-polyclonal-il-17-il-17a-antibody-2711202420171759.jpg "Immunohistochemistry: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Human Lymph Node.Immunohistochemical analysis of paraffin-embedded human lymph node tissue using anti-IL-17/IL-17A antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
![Immunocytochemistry/ Immunofluorescence: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Mouse A-20 Cells.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse A-20 Cells labeling IL-17/IL-17A with at 5 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76337_rabbit-polyclonal-il-17-il-17a-antibody-271120242042535.gif "Immunocytochemistry/ Immunofluorescence: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Mouse A-20 Cells.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse A-20 Cells labeling IL-17/IL-17A with at 5 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).")
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![N/A IL-10 [Biotin]](https://images.novusbio.com/images2/DATA_IL10_DY417_ELISA_2014.jpg)
![SEC-MALS IL-23A/IL-23 P19 [Unconjugated]](https://images.novusbio.com/images/1290-il_recombinant-human-il-23-protein-sec-mals-1012202411154..jpg)
![SDS-PAGE IL-22 [Unconjugated]](https://images.novusbio.com/images2/IL-22_782-IL_CF_734.jpg)
![Bioactivity IL-22 [Unconjugated]](https://images.novusbio.com/images2/IL-22_782-IL_CF_743.jpg)
![Bioactivity IL-4 [Unconjugated]](https://images.novusbio.com/images/protein/6507-ilcf_recombinant-human-il-4-cho-expressed-protein-cf-bioactivity-272020133214.jpg)
![Bioactivity IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFNgamma_285IF_2848.jpg)
![SDS-PAGE IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFN-gamma_285-IF_230.jpg)
![SEC-MALS IFN-gamma [Unconjugated]](https://images.novusbio.com/images/285-if_recombinant-human-ifn-gamma-protein-sec-mals-1520239228..jpg)
![Bioactivity IL-2 [Unconjugated]](https://images.novusbio.com/images/202-il_recombinant-human-il-2-protein-bioactivity-174202314946.jpg)
![N/A IL-17F [Biotin]](https://images.novusbio.com/images2/DATA_IL17F_DY1335_ELISA_2300.jpg)
![N/A IL-17F [Biotin]](https://images.novusbio.com/images2/DATA_IL17F_DY1335_ELISA_1626.jpg)
![N/A IL-37/IL-1F7 [Biotin]](https://images.novusbio.com/images2/DATA_IL37_DY1975_ELISA_1742.jpg)
![N/A IL-13 [Biotin]](https://images.novusbio.com/images2/DATA_IL13_DY413_ELISA_2010.jpg)
![Immunocytochemistry IL-21 Antibody [Unconjugated]](https://images.novusbio.com/images2/IL-21_AF594_Immunocytochemistry_6525.jpg)
![Neutralization IL-21 Antibody [Unconjugated]](https://images.novusbio.com/images2/af594_mouse-il-21-affinity-purified-polyclonal-ab-neutralization-249202192915.jpg)
![N/A IL-5 [HRP]](https://images.novusbio.com/images2/IL-5_M5000_ELISA_237.jpg)
![N/A IL-5 [HRP]](https://images.novusbio.com/images2/DATA_IL5_M5000_ELISA_935.jpg)
![N/A CCL2/JE/MCP-1 [HRP]](https://images.novusbio.com/images2/CCL2_DCP00_ELISA_67.jpg)
![N/A CCL2/JE/MCP-1 [HRP]](https://images.novusbio.com/images2/DATA_CCL2_DCP00_ELISA_633.jpg)
![N/A CCL2/JE/MCP-1 [HRP]](https://images.novusbio.com/images2/DATA_CCL2_DCP00_ELISA_634.jpg)
![Neutralization IL-17RA/IL-17R Antibody (133617) [Unconjugated]](https://images.novusbio.com/images2/IL17_RA_MAB177_Block_Neutralize_15487.jpg)
![Flow Cytometry IL-17RA/IL-17R Antibody (133617) [Unconjugated]](https://images.novusbio.com/images2/IL17_RA_MAB177_CyTOFready_23136.jpg)
![Knockout Validated IL-17RA/IL-17R Antibody (133617) [Unconjugated]](https://images.novusbio.com/images2/IL17_RA_MAB177_Knockout_Validated_23137.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")