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IL-17/IL-17A Antibody (50104) [Unconjugated]

 (121 Publications)
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Western blot shows 25 ng of Recombinant Mouse IL-17/ IL-17A (Catalog # 421-ML), Recombinant Human IL-17/IL-17A (Catalog # 317-ILB), Recombinant Rat IL-17/IL-17A (Catalog # 8410-IL), and Recombinant Mouse IL-17F (Catalog # 2057-IL). PVDF Membrane was probed with 1 µg/mL of Rat Anti-Mouse IL-17/ IL-17A Monoclonal Antibody (Catalog # MAB421) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-17/IL-17A at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Western blot shows 25 ng of Recombinant Mouse IL-17/ IL-17A (Catalog # 421-ML), Recombinant Human IL-17/IL-17A (Catalog # 317-ILB), Recombinant Rat IL-17/IL-17A (Catalog # 8410-IL), and Recombinant Mouse IL-17F (Catalog ...read more
Recombinant Mouse IL‑17 (Catalog # 421-ML) stimulates IL‑6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line), as measured by the Mouse IL‑6 Quantikine ELISA ...read more
Ear histology in WT and Pglyrp-deficient mice in atopic dermatitis and contact dermatitis models of skin inflammation.(A) Oxazolone model of atopic dermatitis: sensitization and 10 applications of oxazolone to the ears ...read more
IL-17 is required for enhanced response to oxazolone in Pglyrp3−/− mice.(A) The level of IL-17-induced chemokine, CXCL-1, is higher in the ears of Pglyrp3−/− mice than WT mice after sensitization and application ...read more
Ear histology in WT and Pglyrp-deficient mice in atopic dermatitis and contact dermatitis models of skin inflammation.(A) Oxazolone model of atopic dermatitis: sensitization and 10 applications of oxazolone to the ears ...read more
Immunogenicity and protective efficacy of BCG + CT – MVA85A.Balb/c mice received BCG±CT i.n. followed by 1×106 CFU MVA85A 10 weeks later. Lungs (A) and spleen (B) were taken at 10 (black circles), 11 (dark grey ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N
Clone
50104
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB421-SP
Carrier Free
MAB421-500
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IL-17/IL-17A Antibody (50104) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse IL‑17
Thr22-Ala158
Accession # Q62386
Specificity
Detects mouse IL-17 in direct ELISAs and Western blots. In direct ELISAs, 100% reactivity with recombinant rat IL-17A and approximately 40% reactivity with recombinant mouse
(rm) IL-17A/IL-17F heterodimer is observed. No cross-reactivity with recombinant human IL-17, recombinant canine IL-17, rmIL-17B,
rmIL‑17C, rmIL-17D, rmIL-17E, or rmIL-17F is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
IL17A
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Neutralization 0.05-0.15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB421 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17/IL-17A Antibody (50104) [Unconjugated]

  • CTLA8
  • CTLA-8
  • CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
  • Cytotoxic T-lymphocyte-associated antigen 8
  • IL17
  • IL-17
  • IL17A
  • IL-17A
  • IL-17Acytotoxic T-lymphocyte-associated protein 8
  • IL-17CTLA-8
  • IL17interleukin-17A
  • interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
  • interleukin 17A

Background

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Mouse IL-17 cDNA encodes a 158 amino acid (aa) residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield the 137 aa residue mature  IL-17. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, mIL-17 shows 57% and 87% sequence identity with herpesvirus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has been cloned. While the expression of IL-17 mRNA is restricted to activated alpha beta TCR+CD4-CD8-T cells, the expression of mIL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-kappa B and costimulation of T cell proliferation.

  1. Kennedy, J. et al. (1996) J. Interferon Cytokine Res. 16:611.
  2. Yao, Z. et al. (1995) J. Immunol. 155:5483.
  3. Yao, Z. et al. (1995) Immunity 3:811.
  4. Rouvier, E. et al. (1993) J. Immunol. 150:5445.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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IL-17/IL-17A
Antibody (50104) [Unc ...
MAB421
IL-17/IL-17A Antibody (50104) [Unconjugated]
Species: Mu
Applications: WB, B/N

Publications for IL-17/IL-17A Antibody (MAB421)(121)

We have publications tested in 3 confirmed species: Mouse, Rat, Bacteria - Porphyromonas gingivalis.

We have publications tested in 9 applications: Blocking, ELISA Development (Capture), ELISpot Development, Flow Cytometry, Functional Assay, ICC, In Vivo, Neutralization, Western Blot.


Filter By Application
Blocking
(1)
ELISA Development (Capture)
(1)
ELISpot Development
(1)
Flow Cytometry
(1)
Functional Assay
(1)
ICC
(1)
In Vivo
(5)
Neutralization
(82)
Western Blot
(4)
All Applications
Filter By Species
Mouse
(90)
Rat
(1)
Bacteria - Porphyromonas gingivalis
(1)
All Species
Showing Publications 1 - 10 of 121. Show All 121 Publications.
Publications using MAB421 Applications Species
Rodríguez-Míguez, Y;Lozano-Ordaz, V;Ortiz-Cabrera, AE;Barrios-Payan, J;Mata-Espinosa, D;Huerta-Yepez, S;Baay-Guzman, G;Hernández-Pando, R; Effect of IL-17A on the immune response to pulmonary tuberculosis induced by high- and low-virulence strains of Mycobacterium bovis PloS one 2024-07-18 [PMID: 39024223] (Neutralization, Mouse) Neutralization Mouse
Niu, D;Yue, SY;Wang, X;Li, WY;Zhang, L;Du, HX;Liang, CZ; High glucose intake exacerbates experimental autoimmune prostatitis through mitochondrial reactive oxygen species-dependent TGF-? activation-mediated Th17 differentiation International immunopharmacology 2024-02-22 [PMID: 38394885] (In Vivo, Mouse) In Vivo Mouse
Ayyaswamy, S;Shi, H;Zhang, B;Bryan, RM;Durgan, DJ; Obstructive Sleep Apnea-Induced Hypertension Is Associated With Increased Gut and Neuroinflammation Journal of the American Heart Association 2023-06-06 [PMID: 37260032] (In Vivo, Rat) In Vivo Rat
BJ Choi, MH Park, KH Park, WH Han, HJ Yoon, HY Jung, JY Hong, MR Chowdhury, KY Kim, J Lee, IS Song, M Pang, MK Choi, E Gulbins, M Reichel, J Kornhuber, CW Hong, C Kim, SH Kim, EH Schuchman, HK Jin, JS Bae Immunotherapy targeting plasma ASM is protective in a mouse model of Alzheimer&#039;s disease Nature Communications, 2023-03-24;14(1):1631. 2023-03-24 [PMID: 36959217] (Neutralization, Mouse) Neutralization Mouse
Rongxin Liao, Peng Zhao, Jianming Wu, Keren Fang Salidroside protects against intestinal barrier dysfunction in septic mice by regulating IL‑17 to block the NF‑ kappa B and p38 MAPK signaling pathways Experimental and Therapeutic Medicine 2023-01-05 [PMID: 36684648]
Bo Wang, Xitan Hou, Yaning Sun, Chao Lei, Sha Yang, Yao Zhu et al. Interleukin-17A influences the vulnerability rather than the size of established atherosclerotic plaques in apolipoprotein E-deficient mice Open Life Sciences 2022-09-08 [PMID: 36133421]
Hasebe R, Murakami K, Harada M et al. ATP spreads inflammation to other limbs through crosstalk between sensory neurons and interneurons The Journal of experimental medicine 6/6/2022 [PMID: 35579694]
D Yu, T Li, JC Delpech, B Zhu, P Kishore, T Koshi, R Luo, KJB Pratt, G Popova, TJ Nowakowski, SA Villeda, X Piao Microglial GPR56 is the molecular target of maternal immune activation-induced parvalbumin-positive interneuron deficits Science Advances, 2022-05-06;8(18):eabm2545. 2022-05-06 [PMID: 35544642] (In Vivo, Mouse) In Vivo Mouse
Diankun Yu, Tao Li, Jean-Christophe Delpech, Beika Zhu, Priya Kishore, Tatsuhiro Koshi et al. Microglial GPR56 is the molecular target of maternal immune activation-induced parvalbumin-positive interneuron deficits Science Advances 2022-05-11 [PMID: 35522745]
Eunha Kim, Donggi Paik, Ricardo N. Ramirez, Delaney G. Biggs, Youngjun Park, Ho-Keun Kwon et al. Maternal gut bacteria drives intestinal inflammation in offspring with neurodevelopmental disorders by altering the chromatin landscape of CD4+ T cells Immunity 2022-01-01 [PMID: 34879222]
Show All 121 Publications.

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Product General Protocols

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FAQs for IL-17/IL-17A Antibody (MAB421). (Showing 1 - 2 of 2 FAQs).

  1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
    • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
  2. I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
    • Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

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Bioinformatics

Gene Symbol IL17A
Uniprot