Home » IL-17/IL-17A » IL-17/IL-17A Antibodies » IL-17/IL-17A Antibody (50101) [Unconjugated]
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IL-17/IL-17A Antibody (50101) [Unconjugated]

 (25 Publications)
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Western blot shows 25 ng of Recombinant Mouse IL-17/IL-17A (Catalog # 421-ML), Recombinant Human IL-17/IL-17A (Catalog # 317-ILB), Recombinant Rat IL-17/IL-17A (Catalog # 8410-IL), and Recombinant Mouse IL-17F (Catalog # 2057-IL). PVDF Membrane was probed with 1 µg/mL of Rat Anti-Mouse IL-17/IL-17A Monoclonal Antibody (Catalog # MAB721) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-17/IL-17A at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Western blot shows 25 ng of Recombinant Mouse IL-17/IL-17A (Catalog # 421-ML), Recombinant Human IL-17/IL-17A (Catalog # 317-ILB), Recombinant Rat IL-17/IL-17A (Catalog # 8410-IL), and Recombinant Mouse IL-17F (Catalog ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA(Cap)
Clone
50101
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB721-SP
Carrier Free
MAB721-500
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IL-17/IL-17A Antibody (50101) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse IL‑17
Thr22-Ala158
Accession # Q62386
Specificity
Detects mouse IL-17 in ELISAs and Western blots. In direct ELISAs, 100% reactivity with recombinant mouse (rm) IL-17A/IL-17F heterodimer, 50% cross-reactivity with recombinant human IL-17, and 25% cross-reactivity with recombinant canine IL-17 is observed. No cross-reactivity with rmIL-17B, rmIL-17C, rmIL-17D, rmIL-17E, or rmIL-17F is observed. In Western blots, cross-reactivity with recombinant rat IL-17A is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
IL17A
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 1 ug/mL
Application Notes
ELISA Detection: Mouse IL-17 Biotinylated Antibody (Catalog number BAF421)
Standard: Recombinant Mouse IL-17 (Catalog number 421-ML)
Publications
Read Publications using
MAB721 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17/IL-17A Antibody (50101) [Unconjugated]

  • CTLA8
  • CTLA-8
  • CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
  • Cytotoxic T-lymphocyte-associated antigen 8
  • IL17
  • IL-17
  • IL17A
  • IL-17A
  • IL-17Acytotoxic T-lymphocyte-associated protein 8
  • IL-17CTLA-8
  • IL17interleukin-17A
  • interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
  • interleukin 17A

Background

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Mouse IL-17 cDNA encodes a 158 amino acid (aa) residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield the 137 aa residue mature  IL-17. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, mouse IL-17 shows 57% and 87% sequence identity with herpes virus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has been cloned. While the expression of IL-17 mRNA is restricted to activated alpha beta TCR+CD4-CD8- T cells, the expression of mouse IL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-kappa B and costimulation of T cell proliferation.

  1. Kennedy, J. et al. (1996) J. Interferon Cytokine Res. 16:611.
  2. Yao, Z. et al. (1995) J. Immunol. 155:5483.
  3. Yao, Z. et al. (1995) Immunity 3:811.
  4. Rouvier, E. et al. (1993) J. Immunol. 150:5445.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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IL-17/IL-17A
Antibody (50101) [Unc ...
MAB721
IL-17/IL-17A Antibody (50101) [Unconjugated]
Species: Mu
Applications: WB, ELISA(Cap)

Publications for IL-17/IL-17A Antibody (MAB721)(25)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 6 applications: ELISA Capture, ELISA Development, ELISA Development (Capture), ELISpot Development, Electrophysiology, Luminex Development.


Filter By Application
ELISA Capture
(1)
ELISA Development
(5)
ELISA Development (Capture)
(8)
ELISpot Development
(4)
Electrophysiology
(1)
Luminex Development
(1)
All Applications
Filter By Species
Mouse
(17)
Rat
(3)
All Species
Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using MAB721 Applications Species
K Kasahara, N Sasaki, HZ Amin, T Tanaka, S Horibe, T Yamashita, KI Hirata, Y Rikitake Depletion of Foxp3+ regulatory T cells augments CD4+ T cell immune responses in atherosclerosis-prone hypercholesterolemic mice Heliyon, 2022-07-19;8(7):e09981. 2022-07-19 [PMID: 35898604] (ELISA Capture, Mouse) ELISA Capture Mouse
M Di Filippo, A Mancini, L Bellingacc, L Gaetani, P Mazzocchet, T Zelante, L La Barbera, A De Luca, M Tantucci, A Tozzi, V Durante, M Sciaccalug, A Megaro, D Chiasserin, N Salvadori, V Lisetti, E Portaccio, C Costa, P Sarchielli, MP Amato, L Parnetti, MT Viscomi, L Romani, P Calabresi Interleukin-17 affects synaptic plasticity and cognition in an experimental model of multiple sclerosis Cell Reports, 2021-12-07;37(10):110094. 2021-12-07 [PMID: 34879272] (Electrophysiology, Mouse) Electrophysiology Mouse
CJ Lee, SJ Moon, JH Jeong, S Lee, MH Lee, SM Yoo, HS Lee, HC Kang, JY Lee, WS Lee, HJ Lee, EK Kim, JY Jhun, ML Cho, JK Min, YY Cho Kaempferol targeting on the fibroblast growth factor receptor 3-ribosomal S6 kinase 2 signaling axis prevents the development of rheumatoid arthritis Cell Death Dis, 2018-03-14;9(3):401. 3/14/2018 [PMID: 29540697]
SH Lee, JY Kwon, SY Kim, K Jung, ML Cho Interferon-gamma regulates inflammatory cell death by targeting necroptosis in experimental autoimmune arthritis Sci Rep, 2017-08-31;7(1):10133. 2017-08-31 [PMID: 28860618] (ELISA Development (Capture), Mouse) ELISA Development (Capture) Mouse
R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3 Cell. Physiol. Biochem., 2017-08-09;42(5):1961-1972. 2017-08-09 [PMID: 28793286] (ELISA Development (Capture), Mouse) ELISA Development (Capture) Mouse
AC Yau, J Tuncel, R Holmdahl The Major Histocompatibility Complex Class III Haplotype Ltab-Ncr3 Regulates Adjuvant-Induced but Not Antigen-Induced Autoimmunity Am. J. Pathol, 2017-03-18;0(0):. 2017-03-18 [PMID: 28315676] (ELISA Development (Capture), Rat) ELISA Development (Capture) Rat
MHC class II alleles associated with Th1 rather than Th17 type immunity drive the onset of early arthritis in a rat model of rheumatoid arthritis Eur. J. Immunol, 2017-02-14;0(0):. 2017-02-14 [PMID: 28012172] (ELISA Development (Capture), Rat) ELISA Development (Capture) Rat
Targeting dendritic cells to accelerate T-cell activation overcomes a bottleneck in tuberculosis vaccine efficacy Nat Commun, 2016-12-22;7(0):13894. 2016-12-22 [PMID: 28004802] (ELISpot Development, Mouse) ELISpot Development Mouse
Jung-Yeon Lim Enhanced immunoregulation of mesenchymal stem cells by IL-10-producing type 1 regulatory T cells in collagen-induced arthritis Sci Rep, 2016-06-01;6(0):26851. 2016-06-01 [PMID: 27246365] (ELISA Development (Capture), Mouse) ELISA Development (Capture) Mouse
Haag S, Tuncel J, Thordardottir S, Mason D, Yau A, Dobritzsch D, Backlund J, Peters E, Holmdahl R Positional identification of RT1-B (HLA-DQ) as susceptibility locus for autoimmune arthritis. J Immunol, 2015-02-11;194(6):2539-50. 2015-02-11 [PMID: 25672758] (ELISA Development (Capture), Rat) ELISA Development (Capture) Rat
Show All 25 Publications.

Reviews for IL-17/IL-17A Antibody (MAB721) (0)

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Product General Protocols

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FAQs for IL-17/IL-17A Antibody (MAB721). (Showing 1 - 2 of 2 FAQs).

  1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
    • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
  2. I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
    • Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

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Bioinformatics

Gene Symbol IL17A
Uniprot