Novus Biologicals products are now on bio-techne.com

IL-17/IL-17A Antibody [Biotin]

Images

 
IL‑17/IL‑17A was detected in immersion fixed paraffin-embedded sections of human Crohn's disease intestine using Goat Anti-Human/Primate IL‑17/IL‑17A Biotinylated Antigen Affinity-purified Polyclonal ...read more

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications IHC, ELISA(Det)
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

IL-17/IL-17A Antibody [Biotin] Summary

Immunogen
E. coli-derived recombinant human IL-17
Ile20-Ala155
Accession # Q16552
Specificity
Detects human and primate IL-17 in direct ELISAs. In sandwich immunoassays, approximately 22% cross-reactivity with rhIL-17A/F heterodimer is observed, and less than 0.05% cross‑reactivity with recombinant mouse (rm) IL‑17, recombinant human (rh) IL-10, rhIL-12, rhIL-16, rhIFN-gamma , rhIL-17B, rhIL-17C, rhIL-17D, rhIL-17E and rhIL-17F is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL17A
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 0.3-15 ug/mL
Application Notes
ELISA Capture: Human IL-17 Antibody (Catalog # MAB317)
ELISA Detection: Human IL-17 Biotinylated Antibody (Catalog # BAF317)
Standard: Recombinant Human IL-17 (Catalog # 317-ILB)
Readout System
Publications
Read Publications using
BAF317 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17/IL-17A Antibody [Biotin]

  • CTLA8
  • CTLA-8
  • CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
  • Cytotoxic T-lymphocyte-associated antigen 8
  • IL17
  • IL-17
  • IL17A
  • IL-17A
  • IL-17Acytotoxic T-lymphocyte-associated protein 8
  • IL-17CTLA-8
  • IL17interleukin-17A
  • interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
  • interleukin 17A

Background

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Human IL-17 cDNA encodes a 155 amino acid (aa) residue precursor protein with a 19 amino acid residue signal peptide that is cleaved to yield the 136 aa residue mature IL-17 containing one potential N-linked glycosylation site. Both recombinant and natural IL-17 have been shown to exist as disulfide-linked homodimers. At the amino acid level, human IL-17 shows 72% and 63% sequence identity with herpesvirus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has been cloned. While the expression of IL-17 mRNA is restricted to activated T cells, the expression of mIL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including the induction of IL-6 and IL-8 production in fibroblasts, the enhancement of surface expression of ICAM-1 in fibroblasts, activation of NF-kappa B and costimulation of T cell proliferation. The non-glycosylated, E. coli-expressed rhIL-17 has been shown to be active on human as well as mouse cell lines.

  1. Yao, Z. et al. (1995) J. Immunol. 155:5483.
  2. Yao, Z. et al. (1995) Immunity 3:811.
  3. Rouvier, E. et al. (1993) J. Immunol. 150:5445.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

M6000B
Species: Mu
Applications: ELISA
DY417
Species: Mu
Applications: ELISA
NBP1-19371
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
1290-IL
Species: Hu
Applications: BA
782-IL/CF
Species: Hu
Applications: BA
6507-IL/CF
Species: Hu
Applications: BA
MAB15041
Species: Hu
Applications: CyTOF-ready, IHC, ICFlow, WB
NB100-39002
Species: Bv, Hu, Mu, Rt
Applications: Dual ISH-IHC, Flow, IB, ICC/IF, IHC, IHC-P, IP, Single-Cell Western, WB
202-IL
Species: Hu
Applications: BA
DY1335B
Species: Hu
Applications: ELISA
DY1975
Species: Hu
Applications: ELISA
DY413
Species: Mu
Applications: ELISA
AF594
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, Neut, WB
M5000
Species: Mu
Applications: ELISA
DCP00
Species: Hu
Applications: ELISA
MAB177
Species: Hu
Applications: CyTOF-ready, Flow, KO, Neut, WB
NBP1-49045
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
BAF317
Species: Hu, Pm
Applications: IHC, ELISA(Det)

Publications for IL-17/IL-17A Antibody (BAF317)(15)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 7 applications: ELISA Capture, ELISA Detection, ELISA Development, ELISA Development (Detection), Functional Assay, IHC-P, Luminex Development.


Filter By Application
ELISA Capture
(2)
ELISA Detection
(2)
ELISA Development
(2)
ELISA Development (Detection)
(3)
Functional Assay
(1)
IHC-P
(1)
Luminex Development
(1)
All Applications
Filter By Species
Human
(12)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using BAF317 Applications Species
L Gorchs, S Ahmed, C Mayer, A Knauf, C Fernández, M Svensson, R Heuchel, E Rangelova, P Bergman, H Kaipe The vitamin D analogue calcipotriol promotes an anti-tumorigenic phenotype of human pancreatic CAFs but reduces T cell mediated immunity Sci Rep, 2020-10-15;10(1):17444. 2020-10-15 [PMID: 33060625] (ELISA Capture, Human) ELISA Capture Human
Nikki P. L. Toledo, Hongzhao Li, Robert W. Omange, Tamara G. Dacoba, Jose Crecente-Campo, Dane Schalk et al. Cervico-Vaginal Inflammatory Cytokine and Chemokine Responses to Two Different SIV Immunogens Frontiers in Immunology 8/25/2020 [PMID: 32983121]
Mehdi Bouhaddou, Danish Memon, Bjoern Meyer, Kris M. White, Veronica V. Rezelj, Miguel Correa Marrero et al. The Global Phosphorylation Landscape of SARS-CoV-2 Infection Cell 8/1/2020 [PMID: 32645325]
J Yan, SP Pandey, BJ Barnes, JR Turner, C Abraham T Cell-Intrinsic IRF5 Regulates T Cell Signaling, Migration, and Differentiation and Promotes Intestinal Inflammation Cell Rep, 2020-06-30;31(13):107820. 2020-06-30 [PMID: 32610123] (ELISA Capture, Human) ELISA Capture Human
Valentin Haug, Branislav Kollar, Sotirios Tasigiorgos, Yori Endo, Martin Kauke, Ali-Farid Safi et al. Hypothermic Ex Situ Perfusion of Human Limbs With Acellular Solution for 24 Hours Transplantation 9/1/2020 [PMID: 32168040]
Y Teng, JL Young, B Edwards, P Hayes, L Thompson, C Johnston, C Edwards, Y Sanders, M Writer, D Pinto, Y Zhang, M Roode, P Chovanec, L Matheson, AE Corcoran, A Fernandez, L Montoliu, B Rossi, V Tosato, K Gjuracic, D Nikitin, C Bruschi, B McGuinness, T Sandal, M Romanos Diverse Human VH antibody fragments with bio-therapeutic properties from the Crescendo Mouse N Biotechnol, 2019-10-07;55(0):65-76. 2019-10-07 [PMID: 31600579] (ELISA Detection, Human) ELISA Detection Human
JC Chamcheu, S Esnault, VM Adhami, AL Noll, S Banang-Mbe, T Roy, SS Singh, S Huang, KG Kousoulas, H Mukhtar Fisetin, a 3,7,3',4'-Tetrahydroxyflavone Inhibits the PI3K/Akt/mTOR and MAPK Pathways and Ameliorates Psoriasis Pathology in 2D and 3D Organotypic Human Inflammatory Skin Models Cells, 2019-09-15;8(9):. 2019-09-15 [PMID: 31540162] (ELISA Detection, Human) ELISA Detection Human
R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3 Cell. Physiol. Biochem., 2017-08-09;42(5):1961-1972. 2017-08-09 [PMID: 28793286] (ELISA Development (Detection), Human) ELISA Development (Detection) Human
E Brencicova, AL Jagger, HG Evans, M Georgouli, A Laios, S Attard Mon, G Mehra, J Spencer, AA Ahmed, S Raju-Kanki, LS Taams, SS Diebold Interleukin-10 and prostaglandin E2 have complementary but distinct suppressive effects on Toll-like receptor-mediated dendritic cell activation in ovarian carcinoma PLoS ONE, 2017-04-14;12(4):e0175712. 2017-04-14 [PMID: 28410380] (ELISA Development (Detection), Human) ELISA Development (Detection) Human
Dobrina N Hull Increase in circulating Th17 cells during anti-TNF therapy is associated with ultrasonographic improvement of synovitis in rheumatoid arthritis Arthritis Res. Ther, 2016-12-23;18(1):303. 2016-12-23 [PMID: 28010726] (ELISA Development (Detection), Human) ELISA Development (Detection) Human
Show All 15 Publications.

Reviews for IL-17/IL-17A Antibody (BAF317) (0)

There are no reviews for IL-17/IL-17A Antibody (BAF317). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for IL-17/IL-17A Antibody (BAF317). (Showing 1 - 2 of 2 FAQs).

  1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
    • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
  2. I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
    • Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our IL-17/IL-17A Antibody [Biotin] and receive a gift card or discount.

Bioinformatics

Gene Symbol IL17A
Uniprot