Home » IL-17/IL-17A » IL-17/IL-17A Antibodies » IL-17/IL-17A Antibody (41809) [Phycoerythrin]
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IL-17/IL-17A Antibody (41809) [Phycoerythrin]

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Human CD4+peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 50 ng/mL PMA, 200 ng/mL Calcium Ionomycin, 200 ng/mL LPS, 20 ng/mL Recombinant Human IL-23 (Catalog # 1290-IL), and 40 ng/mL Recombinant Human IL-6 (Catalog # 206-IL) overnight to induce Th17 development were stained with Mouse Anti-Human IL-17/IL-17A PE-conjugated Monoclonal Antibody (Catalog # IC317P) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC0041P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Human CD4+peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 50 ng/mL PMA, 200 ng/mL Calcium Ionomycin, 200 ng/mL LPS, 20 ng/mL Recombinant Human IL-23 (Catalog # 1290-IL), and 40 ng/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
41809
Clonality
Monoclonal
Host
Mouse
Conjugate
Phycoerythrin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB317U
Alexa Fluor 405
FAB317V
Alexa Fluor 488
IC317G-100UG
Alexa Fluor 532
FAB317X
Alexa Fluor 594
FAB317T
Alexa Fluor 647
FAB317R
Alexa Fluor 700
FAB317N
Alexa Fluor 750
FAB317S
Allophycocyanin
IC317A
Biotin
FAB317B
CoraFluor 1
FAB317CL1
DyLight 350
FAB317D
DyLight 405
FAB317E
DyLight 488
FAB317K
DyLight 550
FAB317L
DyLight 594
FAB317M
DyLight 650
FAB317W
DyLight 680
FAB317Y
DyLight 755
FAB317Z
FITC
FAB317F
HRP
FAB317H
Janelia Fluor 525
FAB317JF525
Janelia Fluor 549
FAB317I
Janelia Fluor 585
FAB317JF585
Janelia Fluor 635
FAB317JF635
Janelia Fluor 646
FAB317J
Janelia Fluor 669
FAB317JF669
PerCP
FAB317C
Unconjugated
MAB317-100
mFluor Violet 450 SE
FAB317MFV450
mFluor Violet 500 SE
FAB317MFV500
mFluor Violet 610 SE
FAB317MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB317-100
Carrier Free
IC317G-100UG
Carrier Free
IC317A
Carrier Free
MAB317-SP
Carrier Free
MAB317-100
Carrier Free
MAB317-500
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IL-17/IL-17A Antibody (41809) [Phycoerythrin] Summary

Immunogen
E. coli-derived recombinant human IL-17/IL-17A
Ile20-Ala155
Accession # Q16552
Specificity
Detects human IL-17/IL-17A in direct ELISAs. In direct ELISAs, approximately 12% cross-reactivity with recombinant canine IL-17/IL-17A is observed and 25%-50% reactivity with recombinant human (rh) IL-17A/IL-17F heterodimer is observed. No cross-reactivity with recombinant mouse IL-17/IL-17A, rhIL-17B, rhIL-17C, rhIL-17D, rhIL-17E, or rhIL-17F is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
IL17A
Purity Statement
Protein A or G purified from ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells
Reviewed Applications
Read 1 Review rated 3
using
IC317P in the following application:
Publications
Read Publication using
IC317P in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17/IL-17A Antibody (41809) [Phycoerythrin]

  • CTLA8
  • CTLA-8
  • CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
  • Cytotoxic T-lymphocyte-associated antigen 8
  • IL17
  • IL-17
  • IL17A
  • IL-17A
  • IL-17Acytotoxic T-lymphocyte-associated protein 8
  • IL-17CTLA-8
  • IL17interleukin-17A
  • interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
  • interleukin 17A

Background

IL-17/IL-17A is a pro-inflammatory cytokine secreted by activated T cells. It is the prototype member of the IL-17 family that also includes IL-17B, C, D, E, and F.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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IL-17/IL-17A
Antibody (41809) [Phy ...
IC317P
IL-17/IL-17A Antibody (41809) [Phycoerythrin]
Species: Hu
Applications: Flow

Publications for IL-17/IL-17A Antibody (IC317P)(1)

We have publications tested in 1 confirmed species: Hamster.

We have publications tested in 1 application: Flow Cytometry.


Filter By Application
Flow Cytometry
(1)
All Applications
Filter By Species
Hamster
(1)
All Species

Review for IL-17/IL-17A Antibody (IC317P) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using IC317P:
Filter by Applications
Flow
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  3
reviewed by:
Verified Customer
 Flow Human 01/27/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID: 21139119
SpeciesHuman

FAQs for IL-17/IL-17A Antibody (IC317P). (Showing 1 - 2 of 2 FAQ).

  1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
    • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
  2. I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
    • Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

Secondary Antibodies

 

Isotype Controls

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Blogs on IL-17/IL-17A

There are no specific blogs for IL-17/IL-17A, but you can read our latest blog posts.
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Recent Reviews

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1
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 Verified Customer
01/27/2015
Application: Flow
Species: Human
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Bioinformatics

Gene Symbol IL17A
Uniprot