![Western Blot: ZW10 Antibody (2E5) [H00009183-M05] - Analysis of ZW10 expression in transfected 293T cell line by ZW10 monoclonal antibody (M05), clone 2E5. Lane 1: ZW10 transfected lysatE (88.8 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ZW10-Antibody-2E5-Western-Blot-H00009183-M05-img0004.jpg "Western Blot: ZW10 Antibody (2E5) [H00009183-M05] - Analysis of ZW10 expression in transfected 293T cell line by ZW10 monoclonal antibody (M05), clone 2E5. Lane 1: ZW10 transfected lysatE (88.8 KDa). Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, KD |
| Clone | 2E5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | ZW10 (NP_004715, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. masfvtevla hsgrlekedl gtrisrltrr veeikgevcn miskkysefl psmqsaqgli tqvdklsedi dllksriese vrrdlhvstg eftdlkqqle |
| Specificity | ZW10 (2E5) |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ZW10 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validation and ELISA. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: ZW10 Antibody (2E5) [H00009183-M05] - Western blot analysis of ZW10 over-expressed 293 cell line, cotransfected with ZW10 Validated Chimera RNAi or non-transfected control. Blot probed with H00009183-M05. GAPDH (36.1 kDa) used as loading control.](http://images.novusbio.com/fullsize/ZW10-Antibody-2E5-Western-Blot-H00009183-M05-img0002.jpg "Western Blot: ZW10 Antibody (2E5) [H00009183-M05] - Western blot analysis of ZW10 over-expressed 293 cell line, cotransfected with ZW10 Validated Chimera RNAi or non-transfected control. Blot probed with H00009183-M05. GAPDH (36.1 kDa) used as loading control.")
![ELISA: ZW10 Antibody (2E5) [H00009183-M05] - Detection limit for recombinant GST tagged ZW10 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ZW10-Antibody-2E5-ELISA-H00009183-M05-img0003.jpg "ELISA: ZW10 Antibody (2E5) [H00009183-M05] - Detection limit for recombinant GST tagged ZW10 is approximately 0.1ng/ml as a capture antibody.")
![Immunocytochemistry Mxi1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Mxi1_AF4185_Immunocytochemistry__Immunofluorescence_20286.jpg)
