![Western Blot: Bub1 Antibody [H00000699-D01P] - Analysis of BUB1 expression in mouse testis.](http://images.novusbio.com/fullsize/Bub1-Antibody-Western-Blot-H00000699-D01P-img0007.jpg "Western Blot: Bub1 Antibody [H00000699-D01P] - Analysis of BUB1 expression in mouse testis.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Func, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody reactive against mammalian transfected lysate. |
| Immunogen | BUB1 (NP_004327.1, 1 a.a. - 1085 a.a.) full-length human protein. MDTPENVLQMLEAHMQSYKGNDPLGEWERYIQWVEENFPENKEYLITLLEHLMKEFLDKKKYHNDPRFISYCLKFAEYNSDLHQFFEFLYNHGIGTLSSPLYIAWAGHLEAQGELQHASAVLQRGIQNQAEPREFLQQQYRLFQTRLTETHLPAQARTSEPLHNVQVLNQMITSKSNPGNNMACISKNQGSELSGVISSACDKESNMERRVITISKSEYSVHSSLASKVDVEQVVMYCKEKLIRGESEFSFEELRAQKYNQRRKHEQWVNEDRHYMKRKEANAFEEQLLKQKMDELHKKLHQVVETSHEDLPASQERSEVNPARMGPSVGSQQELRAPCLPVTYQQTPVNMEKNPREAPPVVPPLANAISAALVSPATSQSIAPPVPLKAQTVTDSMFAVASKDAGCVNKSTHEFKPQSGAEIKEGCETHKVANTSSFHTTPNTSLGMVQATPSKVQPSPTVHTKEALGFIMNMFQAPTLPDISDDKDEWQSLDQNEDAFEAQFQKNVRSSGAWGVNKIISSLSSAFHVFEDGNKENYGLPQPKNKPTGARTFGERSVSRLPSKPKEEVPHAEEFLDDSTVWGIRCNKTLAPSPKSPGDFTSAAQLASTPFHKLPVESVHILEDKENVVAKQCTQATLDSCEENMVVPSRDGKFSPIQEKSPKQALSSHMYSASLLRLSQPAAGGVLTCEAELGVEACRLTDTDAAIAEDPPDAIAGLQAEWMQMSSLGTVDAPNFIVGNPWDDKLIFKLLSGLSKPVSSYPNTFEWQCKLPAIKPKTEFQLGSKLVYVHHLLGEGAFAQVYEATQGDLNDAKNKQKFVLKVQKPANPWEFYIGTQLMERLKPSMQHMFMKFYSAHLFQNGSVLVGELYSYGTLLNAINLYKNTPEKVMPQGLVISFAMRMLYMIEQVHDCEIIHGDIKPDNFILGNGFLEQDDEDDLSAGLALIDLGQSIDMKLFPKGTIFTAKCETSGFQCVEMLSNKPWNYQIDYFGVAATVYCMLFGTYMKVKNEGGECKPEGLFRRLPHLDMWNEFFHVMLNIPDCHHLPSLDLLRQKLKKVFQQHYTNKIRALRNRLIVLLLECKRSRK |
| Specificity | BUB1 - BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast), |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | BUB1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4) |
| Preservative | No Preservative |
| Purity | Protein A purified |
| Publication using H00000699-D01P | Applications | Species |
|---|---|---|
| Serine/threonine Kinase BUB1 Promotes Proliferation and Radio-Resistance in Glioblastoma Yu H, Zhang S, Ibrahim AN et al. Res Pract [PMID: 31272759] (WB, KD, Human) | WB, KD | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for Bub1 Antibody (H00000699-D01P)Find related products by research area.
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| Gene Symbol | BUB1 |
| Entrez |
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| Uniprot |
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![Immunocytochemistry/Immunofluorescence: Bub1 Antibody [H00000699-D01P] - Analysis of antibody to BUB1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Bub1-Antibody-Immunocytochemistry-Immunofluorescence-H00000699-D01P-img0005.jpg "Immunocytochemistry/Immunofluorescence: Bub1 Antibody [H00000699-D01P] - Analysis of antibody to BUB1 on HeLa cell. Antibody concentration 10 ug/ml.")
![Western Blot: Bub1 Antibody [H00000699-D01P] - Analysis of BUB1 expression in Jurkat.](http://images.novusbio.com/fullsize/Bub1-Antibody-Western-Blot-H00000699-D01P-img0006.jpg "Western Blot: Bub1 Antibody [H00000699-D01P] - Analysis of BUB1 expression in Jurkat.")
![Western Blot: Bub1 Antibody [H00000699-D01P] - Analysis of BUB1 expression in transfected 293T cell line by BUB1 polyclonal antibody.Lane 1: BUB1 transfected lysate(122.40 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Bub1-Antibody-Western-Blot-H00000699-D01P-img0008.jpg "Western Blot: Bub1 Antibody [H00000699-D01P] - Analysis of BUB1 expression in transfected 293T cell line by BUB1 polyclonal antibody.Lane 1: BUB1 transfected lysate(122.40 KDa).Lane 2: Non-transfected lysate.")
![Proximity Ligation Assay: Bub1 Antibody [H00000699-D01P] - Analysis of protein-protein interactions between BUB1 and APC. HeLa cells were stained with anti-BUB1 rabbit purified polyclonal 1:1200 and anti-APC mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Bub1-Antibody-Proximity-Ligation-Assay-H00000699-D01P-img0004.jpg "Proximity Ligation Assay: Bub1 Antibody [H00000699-D01P] - Analysis of protein-protein interactions between BUB1 and APC. HeLa cells were stained with anti-BUB1 rabbit purified polyclonal 1:1200 and anti-APC mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).")
![Immunocytochemistry Mxi1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Mxi1_AF4185_Immunocytochemistry__Immunofluorescence_20286.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")