BubR1 Antibody (8G1) - BSA Free

BubR1 (budding uninhibited by benzimidazole-related 1 or mitotic checkpoint serine/threonine-protein kinase BUB1 beta) is an essential component of mitotic checkpoint/control complex which is required for normal mitotic progression and any defects in this complex may result in chromosomal instability/aberrant mitosis. This evolutionarily conserved and crucial system of self-control in cell cycle warrants high quality of chromosomal segregation by postponing manifestation of anaphase in all chromosomes which are incorrectly bi-oriented to mitotic spindles. One of its checkpoint functions is to prevent the activity of anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity; and the other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. BubR1 is required for kinetochore localization of CENPE and it negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. BubR1 is also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest and thus it behaves as a tumor suppressior. Defects in BubR1 have been associated with tumor formation and are the underlying cause of premature chromatid separation trait (PCS) as well as mosaic variegated aneuploidy syndrome type 1 (MVA1).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.

Showing Publications 1 - 8 of 8.

Publications using NB100-353	Applications	Species
Kiseljak-Vassiliades K, Zhang Y, Kar A et al. Elucidating the Role of the Maternal Embryonic Leucine Zipper Kinase (MELK) in Adrenocortical Carcinoma Endocrinology 2018-05-21 [PMID: 29790920] (WB, Human)	WB	Human
Martini S, Soliman T, Gobbi G, Mirandola P et al. PKC epsilon Controls Mitotic Progression by Regulating Centrosome Migration and Mitotic Spindle Assembly Mol. Cancer Res. 2017-10-11 [PMID: 29021232] (ICC/IF, Human)	ICC/IF	Human
Silio V, Redondo-Munoz J, Carrera AC. Phosphoinositide 3-kinase beta regulates chromosome segregation in mitosis Mol Biol Cell 2012-12-01 [PMID: 23051731] (ICC/IF, Human)	ICC/IF	Human
Deeraksa A, Pan J, Sha Y et al. Plk1 is upregulated in androgen-insensitive prostate cancer cells and its inhibition leads to necroptosis. Oncogene 2012-08-01 [PMID: 22890325] (WB, Human)	WB	Human
De Rycker M, Rigoreau L, Dowding S et al. A high-content, cell-based screen identifies micropolyin, a new inhibitor of microtubule dynamics. Chem Biol Drug Des 2009-06-01 [PMID: 19635051] (ICC/IF, Human)	ICC/IF	Human
Bohers E, Sarafan-Vasseur N, Drouet A et al. Gradual reduction of BUBR1 protein levels results in premature sister-chromatid separation then in aneuploidy. Hum Genet 2008-12-01 [PMID: 18932004] (WB, Human)	WB	Human
Trachana, V et al. Dido disruption leads to centrosome amplification and mitotic checkpoint defects compromising chromosome stability. Proc Natl Acad Sci U S A;104(8):2691-6. 2007-02-20 [PMID: 17299043]
Chan, GK et al. Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at kinetochores and binds the cyclosome/APC. J Cell Biol;146(5):941-54. 1999-09-06 [PMID: 10477750]

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Verified Customer	WB	Human	09/06/2021	View Summary Application Western Blot Sample Tested HeLa cell lysates,One-cell embryo Species Human Lot 8G1

Array NB100-353

• BubR1 Antibodies
• BubR1 Lysate
• BubR1 Proteins