![Western Blot: Aurora B Antibody - BSA Free [NB100-294] - Whole extract of PC-12 cells were separated on SDS-PAGE and probed with Anti-Aurora B antibody produced in Rabbit. The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat.Lanes:1. 1:1000 antibody2. 1:2000 antibody3. Negative Control](http://images.novusbio.com/fullsize/Aurora-B-Antibody---BSA-Free-Western-Blot-NB100-294-img0002.jpg "Western Blot: Aurora B Antibody - BSA Free [NB100-294] - Whole extract of PC-12 cells were separated on SDS-PAGE and probed with Anti-Aurora B antibody produced in Rabbit. The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat.Lanes:1. 1:1000 antibody2. 1:2000 antibody3. Negative Control")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP, MA |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 12.5 mg/ml |
| Immunogen | Synthetic peptide corresponding to amino acids 1-19 of human Aurora B with C-terminal added cysteine conjugated to KLH. Uniprot accession no. Q96GD4 |
| Marker | Mitosis Marker |
| Specificity | Aurora B |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | AURKB |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | By Western blot, it detects a band of approximately 41 kDa. Detection of the Aurora B band by immunoblotting is specifically inhibited with the immunizing peptide. |
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| Theoretical MW | 41 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 10mM PBS (pH 7.4) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 12.5 mg/ml |
| Purity | IgG purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
IF | Human | 04/21/2016 |
Summary
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reviewed by:
Verified Customer |
IHC-P | Human | 10/02/2015 |
Summary
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reviewed by:
Andrew Cosgrove |
ICC | Human | 11/06/2013 |
Summary
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reviewed by:
Verified Customer |
IF | Human | 10/04/2013 |
Summary
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reviewed by:
Duane Compton |
IF | Human | 05/02/2012 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Aurora B Antibody (NB100-294)Find related products by research area.
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| Verified Customer 04/21/2016 |
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| Application: | IF | |
| Species: | Human |
| Verified Customer 10/02/2015 |
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| Application: | IHC-P | |
| Species: | Human |
| Andrew Cosgrove 11/06/2013 |
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| Application: | ICC | |
| Species: | Human |
![Immunocytochemistry/Immunofluorescence: Aurora B Antibody - BSA Free [NB100-294] - HeLa human cervix epitheloid carcinoma cells were fixed and permeabilized with 4% paraformaldehyde followed by cold methanol. Fixed cells were stained with 1:50 Anti-Aurora B antibody produced in rabbit. The antibody was developed using Anti-Rabbit IgG (whole molecule)-FITC antibody produced in goat.](http://images.novusbio.com/fullsize/Aurora-B-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-294-img0001.jpg "Immunocytochemistry/Immunofluorescence: Aurora B Antibody - BSA Free [NB100-294] - HeLa human cervix epitheloid carcinoma cells were fixed and permeabilized with 4% paraformaldehyde followed by cold methanol. Fixed cells were stained with 1:50 Anti-Aurora B antibody produced in rabbit. The antibody was developed using Anti-Rabbit IgG (whole molecule)-FITC antibody produced in goat.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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