MAD2L1 Antibody

The spindle checkpoint ensures the fidelity of chromosome segregation in mitosis and meiosis. In response to defects in the mitotic apparatus, it blocks the activity of the anaphase-promoting complex, a large ubiquitin ligase required for chromosome segregation. Recent studies indicate that the spindle checkpoint monitors both the attachment of chromosomes to the mitotic spindle and the tension across the sister chromatid generated by microtubules. Upon checkpoint activation, checkpoint protein complexes containing BubR1(Mad3), Bub3, Mad2 and Cdc20 directly bind to the anaphase-promoting complex and inhibit its ligase activity. Therefore, the checkpoint proteins form a complex intracellular signalling network to inhibit the anaphase-promoting complex.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.

Showing Publications 1 - 10 of 14. Show All 14 Publications. Collapse Publications.

Publications using NB100-563	Applications	Species
Itoh Go, Ikeda Masanori, Iemura Kenji et al. Lateral attachment of kinetochores to microtubules is enriched in prometaphase rosette and facilitates chromosome alignment and bi-orientation establishment. Medicine 2018-03-01 [PMID: 29497093] (ICC/IF, Human)	ICC/IF	Human
Ikeda M, Tanaka K. Plk1 bound to Bub1 contributes to spindle assembly checkpoint activity during mitosis Sci Rep 2017-08-18 [PMID: 28821799] (WB, Human)	WB	Human
Guccini S, Lombardi S, Pisani A et al. Effects of spindle poisons in peripheral human lymphocytes by the in vitro cytokinesis-block micronucleus assay. Mutagenesis 2012-11-01 [PMID: 22952150]
Matson DR, Demirel PB, Stukenberg PT, Burke DJ. A conserved role for COMA/CENP-H/I/N kinetochore proteins in the spindle checkpoint. FEBS J. 2012-03-16 [PMID: 22426531]
McHedlishvili N, Wieser S, Holtackers R et al. Kinetochores accelerate centrosome separation to ensure faithful chromosome segregation. J Cell Sci 2012-02-01 [PMID: 22399803]
Klebig C, Korinth D, Meraldi P et al. Bub1 regulates chromosome segregation in a kinetochore-independent manner. J Cell Biol 2009-06-01 [PMID: 19487456]
Chan YW, Fava LL, Uldschmid A et al. Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly. J Cell Biol. 2009-06-01 [PMID: 19468067]
Yun M, Han YH, Yoon SH et al. p31comet Induces cellular senescence through p21 accumulation and Mad2 disruption. Mol Cancer Res 2009-03-01 [PMID: 19276188]
Lince-Faria M, Maffini S, Orr B et al. Spatiotemporal control of mitosis by the conserved spindle matrix protein Megator. J Cell Biol 2009-03-01 [PMID: 19273613]
Vader G, Cruijsen CW, van Harn T et al. The chromosomal passenger complex controls spindle checkpoint function independent from its role in correcting microtubule kinetochore interactions. Mol Biol Cell 2007-11-01 [PMID: 17699588]
Santamaria A, Neef R, Eberspacher U et al. Use of the novel Plk1 inhibitor ZK-thiazolidinone to elucidate functions of Plk1 in early and late stages of mitosis. Mol Biol Cell 2007-10-01 [PMID: 17671160]
Agoston AT, Argani P, De Marzo AM et al. Retinoblastoma pathway dysregulation causes DNA methyltransferase 1 overexpression in cancer via MAD2-mediated inhibition of the anaphase-promoting complex. Am J Pathol 2007-05-01 [PMID: 17456764]
Wu, G, Glickstein, S, Liu, W, Fujita, T, Li, W, Yang, Q, Duvoisin, R, Wan, Y. The Anaphase-promoting Complex Coordinates Initiation of Lens Differentiation, . 18, 1018-1029. 2007-01-01 [PMID: 17215516]
Ito S, Mantel CR, Han MK et al. Mad2 is required for optimal hematopoiesis: Mad2 associates with c-Kit in MO7e cells. Blood 2007-03-01 [PMID: 17038523]
Show All 14 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Verified Customer	WB	Human	08/27/2014	View Summary Application Western Blot Sample Tested Whole cell lysate from HeLa cells Species Human

Array NB100-563

• MAD2L1 Antibodies
• MAD2L1 Lysate
• MAD2L1 Proteins