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ZWILCH Antibody

Images

 
Western Blot: ZWILCH Antibody [NBP2-21057] - Sample (30 ug of whole cell lysate) A: MCF-7 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: ZWILCH Antibody [NBP2-21057] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: ZWILCH Antibody [NBP2-21057] - Paraffin-embedded human breast carcinoma. ZWILCH antibody diluted at 1:500.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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ZWILCH Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ZWILCH. The exact sequence is proprietary.
Localization
Kinetochore
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ZWILCH
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse (81%), Bovine (85%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for ZWILCH Antibody

  • dJ337O18.5
  • SWIM domain containing 1
  • zinc finger, SWIM-type containing 1

Background

Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ZWILCH Antibody (NBP2-21057) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ZWILCH
Uniprot