![Western Blot: XRCC4 Antibody (OTI4H9) - Azide and BSA Free [NBP2-74896] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC4.](http://images.novusbio.com/fullsize/XRCC4-Antibody-OTI4H9-Azide-and-BSA-Free-Western-Blot-NBP2-74896-img0001.jpg "Western Blot: XRCC4 Antibody (OTI4H9) - Azide and BSA Free [NBP2-74896] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC4.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clone | OTI4H9 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full-length protein expressed in 293T cell transfected with human XRCC4 expression vector |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | XRCC4 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 38.3 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
Secondary Antibodies |
Isotype Controls |
Research Areas for XRCC4 Antibody (NBP2-74896)Find related products by research area.
|
|
Ku70: The DNA's Mr. Fix-it Ku70, known by several synonyms including X-ray repair cross-complementing, 5'-deoxyribose-5-phosphate lyase Ku70 protein 6, 70 kDa subunit of Ku antigen, XRCC6, and G22P1, is a 70 kDa protein that was shown to be involved in multiple cellular pathway... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | XRCC4 |
![Immunohistochemistry: XRCC4 Antibody (OTI4H9) - Azide and BSA Free [NBP2-74896] - Staining of paraffin-embedded lung tissue using anti-XRCC4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XRCC4-Antibody-OTI4H9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74896-img0006.jpg "Immunohistochemistry: XRCC4 Antibody (OTI4H9) - Azide and BSA Free [NBP2-74896] - Staining of paraffin-embedded lung tissue using anti-XRCC4 mouse monoclonal antibody.")
![Immunohistochemistry: XRCC4 Antibody (OTI4H9) - Azide and BSA Free [NBP2-74896] - Staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-XRCC4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XRCC4-Antibody-OTI4H9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74896-img0002.jpg "Immunohistochemistry: XRCC4 Antibody (OTI4H9) - Azide and BSA Free [NBP2-74896] - Staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-XRCC4 mouse monoclonal antibody.")
![Immunohistochemistry: XRCC4 Antibody (OTI4H9) - Azide and BSA Free [NBP2-74896] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-XRCC4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XRCC4-Antibody-OTI4H9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74896-img0003.jpg "Immunohistochemistry: XRCC4 Antibody (OTI4H9) - Azide and BSA Free [NBP2-74896] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-XRCC4 mouse monoclonal antibody.")
![Immunohistochemistry: XRCC4 Antibody (OTI4H9) - Azide and BSA Free [NBP2-74896] - Staining of paraffin-embedded colon tissue using anti-XRCC4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XRCC4-Antibody-OTI4H9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74896-img0004.jpg "Immunohistochemistry: XRCC4 Antibody (OTI4H9) - Azide and BSA Free [NBP2-74896] - Staining of paraffin-embedded colon tissue using anti-XRCC4 mouse monoclonal antibody.")
![Immunohistochemistry: XRCC4 Antibody (OTI4H9) - Azide and BSA Free [NBP2-74896] - Staining of paraffin-embedded Kidney tissue using anti-XRCC4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XRCC4-Antibody-OTI4H9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74896-img0005.jpg "Immunohistochemistry: XRCC4 Antibody (OTI4H9) - Azide and BSA Free [NBP2-74896] - Staining of paraffin-embedded Kidney tissue using anti-XRCC4 mouse monoclonal antibody.")
![Immunohistochemistry: XRCC4 Antibody (OTI4H9) - Azide and BSA Free [NBP2-74896] - Staining of paraffin-embedded thyroid tissue using anti-XRCC4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XRCC4-Antibody-OTI4H9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74896-img0007.jpg "Immunohistochemistry: XRCC4 Antibody (OTI4H9) - Azide and BSA Free [NBP2-74896] - Staining of paraffin-embedded thyroid tissue using anti-XRCC4 mouse monoclonal antibody.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")