![Western Blot: DNA Ligase IV Antibody [NBP2-16182] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody diluted at 1:500.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Western-Blot-NBP2-16182-img0005.jpg "Western Blot: DNA Ligase IV Antibody [NBP2-16182] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody diluted at 1:500.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human DNA Ligase IV. The exact sequence is proprietary. |
| Localization | Nucleus |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | LIG4 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Theoretical MW | 104 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
|
| Publications |
|
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
| Publication using NBP2-16182 | Applications | Species |
|---|---|---|
| Hu HL, Shiflett LA, Kobayashi M et al. TOP2b-Dependent Nuclear DNA Damage Shapes Extracellular Growth Factor Responses via Dynamic AKT Phosphorylation to Control Virus Latency Mol. Cell 2019-03-26 [PMID: 30930055] (WB, Rat) | WB | Rat |
Secondary Antibodies |
Isotype Controls |
Research Areas for DNA Ligase IV Antibody (NBP2-16182)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: DNA Ligase IV Antibody [NBP2-16182] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16182-img0003.jpg "Immunocytochemistry/Immunofluorescence: DNA Ligase IV Antibody [NBP2-16182] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP2-16182] - Human ovarian cancer, using DNA ligase IV antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Immunohistochemistry-Paraffin-NBP2-16182-img0002.jpg "Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP2-16182] - Human ovarian cancer, using DNA ligase IV antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: DNA Ligase IV Antibody [NBP2-16182] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Western-Blot-NBP2-16182-img0001.jpg "Western Blot: DNA Ligase IV Antibody [NBP2-16182] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: DNA Ligase IV Antibody [NBP2-16182] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody diluted at 1:500.](http://images.novusbio.com/fullsize/DNA-Ligase-IV-Antibody-Western-Blot-NBP2-16182-img0004.jpg "Western Blot: DNA Ligase IV Antibody [NBP2-16182] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody diluted at 1:500.")
![Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP2-16182] -Rat colon.DNA ligase IV stained by DNA ligase IV antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/antibody/nbp2-16182_rabbit-polyclonal-dna-ligase-iv-antibody-immunohistochemistry-paraffin-992024123530..jpg "Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP2-16182] -Rat colon.DNA ligase IV stained by DNA ligase IV antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunocytochemistry/Immunofluorescence: DNA Ligase IV Antibody [NBP2-16182] -MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DNA ligase IV stained by DNA ligase IV antibody diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/antibody/nbp2-16182_rabbit-polyclonal-dna-ligase-iv-antibody-immunocytochemistry-immunofluorescence-992024123725..jpg "Immunocytochemistry/Immunofluorescence: DNA Ligase IV Antibody [NBP2-16182] -MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DNA ligase IV stained by DNA ligase IV antibody diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP2-16182] - DNA Ligase IV antibody detects DNA Ligase IV protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat colon.DNA Ligase IV stained by DNA Ligase IV antibody (NBP2-16182) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16182_rabbit-polyclonal-dna-ligase-iv-antibody-910202420134047.jpg "Immunohistochemistry-Paraffin: DNA Ligase IV Antibody [NBP2-16182] - DNA Ligase IV antibody detects DNA Ligase IV protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat colon.DNA Ligase IV stained by DNA Ligase IV antibody (NBP2-16182) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunocytochemistry/ Immunofluorescence: DNA Ligase IV Antibody [NBP2-16182] - DNA Ligase IV antibody detects DNA Ligase IV protein at nucleus and nucleolus by immunofluorescent analysis.Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DNA Ligase IV stained by DNA Ligase IV antibody (NBP2-16182) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-16182_rabbit-polyclonal-dna-ligase-iv-antibody-91020242041277.jpg "Immunocytochemistry/ Immunofluorescence: DNA Ligase IV Antibody [NBP2-16182] - DNA Ligase IV antibody detects DNA Ligase IV protein at nucleus and nucleolus by immunofluorescent analysis.Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DNA Ligase IV stained by DNA Ligase IV antibody (NBP2-16182) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .")
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_6532.jpg)
![Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase3_AF835_Immunohistochemistry_22976.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_9340.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")