Reactivity | Hu, Pm, Ch(-), Mu(-), Rt(-)Species Glossary |
Applications | Flow, ICC/IF |
Clone | KU729 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Unconjugated |
Concentration | 0.2 mg/ml |
Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34663) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
Immunogen | Nuclear extract of human HL-60 cells |
Localization | Nuclear |
Marker | Nuclear Marker |
Specificity | Recognizes a dimer of two proteins of 70kDa and ~80kDa, identified as two subunits of Ku. This monoclonal antibody recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis. |
Isotype | IgG1 Kappa |
Clonality | Monoclonal |
Host | Mouse |
Gene | XRCC6 |
Purity | Protein A or G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Immunocytochemistry (Acetone-fixed cells): 0.5-1.0ug/ml for 30 minutes at RT. Optimal dilution for a specific application should be determined. Optimal dilution for a specific application should be determined. |
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Reviewed Applications |
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Storage | Store at 4C. |
Buffer | 10 mM PBS with 0.05% BSA |
Preservative | 0.05% Sodium Azide |
Concentration | 0.2 mg/ml |
Purity | Protein A or G purified |
Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
ICC | Human | 03/22/2022 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Ku70/XRCC6 Antibody (NBP2-34247)Find related products by research area.
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