Rad51 Antibody (14B4)

Rad51, a 37 kDa protein, is the human homolog of E. coli RecA protein and a member of the RAD52 epistasis group in S. cerevisiae. In recent studies it has been reported that BRCA1 interacts with Rad51 and disease-causing mutations have been found in the BRCA1 region necessary for BRCA1/Rad51 interaction, implying that this interaction is important for tumor suppression. BRCA2 and Rad51 have also been shown to interact by direct binding of the BRC repeats located within exon 11 of BRCA2. When these repeats are deleted, the interaction is lost and cells become hypersensitive to the DNA damage caused by methyl methanesulfonate (MMS).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, C. elegans.

We have publications tested in 7 applications: ChIP, Chemotaxis, ICC/IF, IF/IHC, IHC-P, In vitro, WB.

Showing Publications 1 - 10 of 64. Show All 64 Publications. Collapse Publications.

Publications using NB100-148	Applications	Species
Davies B, Zhang G, Moralli D et al. Characterization of meiotic recombination intermediates through gene knockouts in founder hybrid mice Genome research 2023-11-17 [PMID: 37977820] Details: Sample type: Testis
Ito M, Furukohri A, Matsuzaki K et al. FIGNL1 AAA+ ATPase remodels RAD51 and DMC1 filaments in pre-meiotic DNA replication and meiotic recombination Nature communications 2023-10-27 [PMID: 37891173] (ChIP, Mouse)	ChIP	Mouse
Shell DJ, Foley CA, Wang Q et al. Discovery of a 53BP1 Small Molecule Antagonist Using a Focused DNA-Encoded Library Screen Journal of medicinal chemistry 2023-10-26 [PMID: 37782247] (Human)		Human
Neizer-Ashun F, Dwivedi SKD, Dey A et al. KRCC1, a modulator of the DNA damage response Nucleic acids research 2022-10-16 [PMID: 36243983] (WB)	WB
Kiyga E, Adıguzel Z, Onay Ucar E Temozolomide increases heat shock proteins in extracellular vesicles released from glioblastoma cells Molecular biology reports 2022-06-25 [PMID: 35752701] (WB, Human)	WB	Human
Zhou Z, Yin H, Suye S et al. The changes of DNA double-strand breaks and DNA repair during ovarian reserve formation in mice Reproductive biology 2022-01-10 [PMID: 35026551] (IF/IHC, Mouse)	IF/IHC	Mouse
Fernando M, Duijf PHG, Proctor M et al. Dysregulated G2 phase checkpoint recovery pathway reduces DNA repair efficiency and increases chromosomal instability in a wide range of tumours Oncogenesis 2021-05-15 [PMID: 33993200] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Veo BL, Danis E, Pierce A et al. Transcriptional control of DNA repair networks by CDK7 regulates sensitivity to radiation in Myc-driven Medulloblastoma Cell Rep 2021-04-28 [PMID: 33910002]
Ma, L, Ruan, J Et al. MiCas9 increases large size gene knock-in rates and reduces undesirable on-target and off-target indel edits. Nat Commun 2020-11-27 [PMID: 33247137] (WB, Mouse)	WB	Mouse
GarcIa-Muse T Detection of DSBs in C. elegans Meiosis Methods Mol. Biol. 2020-08-26 [PMID: 32840787]
Sriramkumar S, Matthews TD, Ghobashi AH, Miller SA Platinum-Induced Ubiquitination of Phosphorylated H2AX by RING1A is Mediated by Replication Protein A in Ovarian Cancer bioRxiv Jan 1 2020 (WB, Human)	WB	Human
Kaminski P, Hong S, Kono T, et al. Topoisomerase 2 beta induces DNA breaks to regulate human papillomavirus replication bioRxiv 2021-01-10 [PMID: 33563836]
Dong C, An L, Yu CH, Huen MSY A DYRK1B-dependent pathway suppresses rDNA transcription in response to DNA damage Nucleic acids research 2021-01-19 [PMID: 33469661] (ICC/IF)	ICC/IF
Correll-Tash S, Lilley B, Harold Salmons IV et al. Double Strand Breaks (DSBs) as Indicators of Genomic Instability in PATRR-mediated Translocations Human molecular genetics 2020-12-01 [PMID: 33258468]
Sriramkumar, S, Matthews, T D Et al. Platinum-Induced Ubiquitination of Phosphorylated H2AX by RING1A Is Mediated by Replication Protein A in Ovarian Cancer. Mol Cancer Res 2020-11-01 [PMID: 32801161] (ICC/IF, Human)	ICC/IF	Human
Hinch AG, Becker PW, Li T et al. The Configuration of RPA, RAD51, and DMC1 Binding in Meiosis Reveals the Nature of Critical Recombination Intermediates Mol. Cell 2020-06-23 [PMID: 32610038]
Kono T, Hoover P, Poropatich K et al. Activation of DNA damage repair factors in HPV positive oropharyngeal cancers Virology 2020-01-01 [PMID: 32560902] (IHC-P, Human)	IHC-P	Human
Feng W, Simpson DA, Carvajal-Garcia J et al. Genetic determinants of cellular addiction to DNA polymerase theta Nat Commun [PMID: 31537809] (ICC/IF, Mouse)	ICC/IF	Mouse
Tu Z, Mu X, Chen X, et al. Dibutyl phthalate exposure disrupts the progression of meiotic prophase I by interfering with homologous recombination in fetal mouse oocytes Environ. Pollut. 2019-05-24 [PMID: 31158667] (ICC/IF, Mouse)	ICC/IF	Mouse
Deveryshetty, J;Peterlini, T;Ryzhikov, M;Brahiti, N;Dellaire, G;Masson, JY;Korolev, S; Novel RNA and DNA strand exchange activity of the PALB2 DNA Binding Domain and its critical role for DNA repair in cells Elife 2019-04-24 [PMID: 31017574] (WB, Human)	WB	Human
Shin N, Cuenca L, Karthikraj R et al. Assessing effects of germline exposure to environmental toxicants by high-throughput screening in C. elegans PLoS Genet. 2019-02-01 [PMID: 30763314] (ICC/IF, C. elegans)	ICC/IF	C. elegans
Garcia-Muse T, Galindo-Diaz U, Garcia-Rubio M et al. A Meiotic Checkpoint Alters Repair Partner Bias to Permit Inter-sister Repair of Persistent DSBs. Cell Rep 2019-01-15 [PMID: 30650366] (IF/IHC, C. elegans)	IF/IHC	C. elegans
Sulkowski PL, Sundaram RK, Oeck S et al. Krebs-cycle-deficient hereditary cancer syndromes are defined by defects in homologous-recombination DNA repair. Nat. Genet. 2018-08-01 [PMID: 30013182] (WB, Human)	WB	Human
PeriaSamy J, Kurdekar V, Jasti S et al. Targeting Phosphopeptide Recognition by the Human BRCA1 Tandem BRCT Domain to Interrupt BRCA1-Dependent Signaling. Cell Chem Biol 2018-03-09 [PMID: 29606576] (ICC/IF)	ICC/IF
Scanlon SE, Hegan DC, Sulkowski PL, Glazer PM. Suppression of homology-dependent DNA double-strand break repair induces PARP inhibitor sensitivity inVHL-deficient human renal cell carcinoma. Oncotarget. 2018-01-12 [PMID: 29435132] (WB, Human)	WB	Human
Li Q, Xie W, Wang N et al. CDC7-dependent transcriptional regulation of RAD54L is essential for tumorigenicity and radio-resistance of glioblastoma. Transl Oncol 2018-04-01 [PMID: 29413763]
Ha K, Ma C, Lin H et al. The anaphase promoting complex impacts repair choice by protecting ubiquitin signalling at DNA damage sites. Nat Commun. 2017-06-12 [PMID: 28604711] (ICC/IF, Human)	ICC/IF	Human
Nadarajan S, Lambert TJ, Altendorfer E et al. Polo-like kinase-dependent phosphorylation of the synaptonemal complex protein SYP-4 regulates double-strand break formation through a negative feedback loop. Elife. 2017-03-27 [PMID: 28346135] (ICC/IF, C. elegans)	ICC/IF	C. elegans
Pauty J, Couturier AM, Rodrigue A et al. Cancer-causing mutations in the tumor suppressor PALB2 reveal a novel cancer mechanism using a hidden nuclear export signal in the WD40 repeat motif. Nucleic Acids Res. 2017-03-17 [PMID: 28158555]
Ackermann L, Schell M, Pokrzywa W et al. E4 ligase-specific ubiquitination hubs coordinate DNA double-strand-break repair and apoptosis Nat. Struct. Mol. Biol. 2016-09-26 [PMID: 27669035] (IF/IHC, C. elegans)	IF/IHC	C. elegans
Neizer-Ashun F, Dwivedi S, Dey A et al. KRCC1, a novel modulator of the DNA damage response Research Square 2022-04-08 (WB)	WB
Phillips AF, Millet AR, Tigano M et al. Single-Molecule Analysis of mtDNA Replication Uncovers the Basis of the Common Deletion Mol. Cell 2017-01-11 [PMID: 28111015]
Fernando P Targeting DNA damage response defects in cancers The University of Queensland 2022-01-21
Li M, Yu X, Chen Q. Chemopreventive effects of ROS targeting in a murine model of BRCA1-deficient breast cancer. Cancer Res. 2016-11-04 [PMID: 27815389] (In vitro, Human)	In vitro	Human
Dok R, Abbasi Asbagh L, Van Limbergen EJ et al. Nuclear p16INK4a expression predicts enhanced radiation response in head and neck cancers. Oncotarget 2016-05-26 [PMID: 27246975] (IF/IHC, Human)	IF/IHC	Human
Liu H, Yan P, Fanning E. Human DNA helicase B functions in cellular homologous recombination and stimulates rad51-mediated 5'-3' heteroduplex extension in vitro PLoS ONE. 2015-01-25 [PMID: 25617833] (ICC/IF, Human) Details: RAD52 antibody used for ICC-IF on U2OS cells which were transfected with human DNA helicase B/HDHB. The immunostaining assay involved - treatment of cells with 5 Gy IR radiation, extraction of cells with 0.2% Triton X-100 in CSK buffer, fixation of cells with 3.7 % PFA at RT for 20 minutes, primary antibody incubation at 1:300 dilution in PBS-10%FBS, detection with IF labelled secondary antibody (Figure 3)	ICC/IF	Human
Cornago M, Garcia-Alberich C, Blasco-Angulo N et al. Histone deacetylase inhibitors promote glioma cell death by G2 checkpoint abrogation leading to mitotic catastrophe. Cell Death Dis. 2014-10-03 [PMID: 25275596] (WB, Human) Details: Survivin antibody used for ICC-IF on U251-MG cells in metaphase after HDAC inhibitor suberanilohydroxamic acid/SAHA treatment (Figure 7a). Antibody also used for WB on lysates of glioma cell lines U87, U251-MG, LN229 cells as well as human GBM primary cultures C55, C65, C48 and C60 treated or not with HDAC inhibitor SAHA and valproic acid/VPA (Figure 7b and c)	WB	Human
Del Nagro Christopher J, Choi Jonathan, Xiao Yang et al. Chk1 inhibition in p53-deficient cell lines drives rapid chromosome fragmentation followed by caspase-independent cell death. Cell Cycle. 2014-01-01 [PMID: 24247149] (ICC/IF, Human)	ICC/IF	Human
Wu X, Xu Y, Feng K et al. MutS Homologue hMSH5: Recombinational DSB Repair and Non-Synonymous Polymorphic Variants. PLoS One. 2013-09-04 [PMID: 24023853] (Chemotaxis, Human)	Chemotaxis	Human
Lans H, Lindvall JM, Thijssen K et al. DNA damage leads to progressive replicative decline but extends the life span of long-lived mutant animals. Cell Death Differ. 2013-09-06 [PMID: 24013725] (ICC/IF, IF/IHC, C. elegans)	ICC/IF, IF/IHC	C. elegans
Buonanno M, Garty G, Grad M et al. Microbeam irradiation of C. elegans nematode in microfluidic channels. Radiat Environ Biophys. 2013-08-13 [PMID: 23942865] (IF/IHC, ICC/IF, C. elegans)	IF/IHC, ICC/IF	C. elegans
Chu YL, Wu X, Xu Y, Her C. MutS homologue hMSH4: interaction with eIF3f and a role in NHEJ-mediated DSB repair. Mol Cancer 2013-06-02 [PMID: 23725059] (WB, Human)	WB	Human
Tompkins JD, Wu X, Her C. MutS homologue hMSH5: role in cisplatin-induced DNA damage response. Mol Cancer;11:10. 2012-03-08 [PMID: 22401567] (WB, Human)	WB	Human
Moldovan G-L, Dejsuphong D, Petalcorin MIR et al. Inhibition of Homologous Recombination by the PCNA-Interacting Protein PARI. Molecular Cell. 2011-12-06 [PMID: 22153967]
Meerang M, Ritz D, Paliwal S et al. The ubiquitin-selective segregase VCP/p97 orchestrates the response to DNA double-strand breaks. Nat Cell Biol;13(11):1376-82. 2011-10-23 [PMID: 22020440]
Harper NC, Rillo R, Jover-Gil S et al. Pairing Centers Recruit a Polo-like Kinase to Orchestrate Meiotic Chromosome Dynamics in C. elegans Developmental Cell. 2011-10-19 [PMID: 22018922]
Lee M, Daniels MJ, Garnett MJ et al. A mitotic function for the high-mobility group protein HMG20b regulated by its interaction with the BRC repeats of the BRCA2 tumor suppressor. Oncogene 2011-07-01 [PMID: 21399666] (ICC/IF)	ICC/IF
Klammer H, Kadhim M, Iliakis G et al. Evidence of an adaptive response targeting DNA nonhomologous end joining and its transmission to bystander cells. Cancer Res 2010-11-01 [PMID: 20861183] (ICC/IF)	ICC/IF
Ammazzalorso F, Pirzio LM, Bignami M et al. ATR and ATM differently regulate WRN to prevent DSBs at stalled replication forks and promote replication fork recovery. EMBO J 2010-09-01 [PMID: 20802463] (ICC/IF)	ICC/IF
Graeser M, McCarthy A, Lord CJ et al. A marker of homologous recombination predicts pathologic complete response to neoadjuvant chemotherapy in primary breast cancer. Clin Cancer Res 2010-12-01 [PMID: 20802015] (ICC/IF)	ICC/IF
Bowen C, Gelmann EP. NkX3.1 activates cellular response to DNA damage. Cancer Res 2010-04-01 [PMID: 20395202] (ICC/IF)	ICC/IF
Suzuki K, Yamauchi M, Oka Y et al. A novel and simple micro-irradiation technique for creating localized DNA double-strand breaks. Nucleic Acids Res 2010-07-01 [PMID: 20385602] (ICC/IF)	ICC/IF
Moldovan GL, Madhavan MV, Mirchandani KD et al. DNA polymerase POLN participates in crosslink repair and homologous recombination. Mol Cell Biol 2009-12-07 [PMID: 19995904]
Dungey FA, Caldecott KW, Chalmers AJ. Enhanced radiosensitization of human glioma cells by combining inhibition of poly(ADP-ribose) polymerase with inhibition of heat shock protein 90. Mol Cancer Ther 2009-08-01 [PMID: 19671736] (ICC/IF)	ICC/IF
Carlessi L, De Filippis L, Lecis D et al. DNA-damage response, survival and differentiation in vitro of a human neural stem cell line in relation to ATM expression. Cell Death Differ 2009-06-01 [PMID: 19229246] (ICC/IF)	ICC/IF
Stewart GS, Stankovic T, Byrd PJ et al. RIDDLE immunodeficiency syndrome is linked to defects in 53BP1-mediated DNA damage signaling. Proc Natl Acad Sci U S A 2007-10-01 [PMID: 17940005]
Shim, K et al. hXRCC2 enhances ADP/ATP processing and strand exchange by hRAD51. J Biol Chem;279(29):30385-94. 2004-07-16 [PMID: 15123651]
Warren M, Lord CJ, Masabanda J et al. Phenotypic effects of heterozygosity for a BRCA2 mutation. Hum Mol Genet 2003-10-01 [PMID: 12928478] (ICC/IF)	ICC/IF
Kao GD, McKenna WG, Guenther MG et al. Histone deacetylase 4 interacts with 53BP1 to mediate the DNA damage response. J Cell Biol 2003-03-01 [PMID: 12668657] (ICC/IF)	ICC/IF
Tarsounas, N et al. BRCA2-dependent independent formation of RAD51 nuclear foci. Oncogene. 22:1115-1123. 2003-01-01 [PMID: 12606939]
French, CA et al. Role of mammalian RAD51L2 (RAD51C) in recombination genetic stability. J Biol Chem 277:19322-19330. 2002-01-01 [PMID: 11912211]
Wiese C, Collins DW, Albala JS et al. Interactions involving the Rad51 paralogs Rad51C and XRCC3 in human cells. Nucleic Acids Res 2002-02-15 [PMID: 11842112]
Masson, J et al. Identification purification of two distinct complexes containing the five Rad51 paralogs. Genes Dev 15:3296-3207. 2001-01-01 [PMID: 11751635]
Masson, J et al. The meiosis-specific recombinase hDms1 forms ring structures interacts with hRad51. EMBO J. 18:6552-6560. 1999-01-01 [PMID: 10562567]
Show All 64 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Marina Rodriguez Candela	ICC	Human	06/14/2021	View Summary Application Immunocytochemistry Sample Tested human immortalized breast cancer cells Species Human Lot 43574 Comments Comments Cells were fixed with 4% PFA. RAD51 Ig was diluted 1:200.
Enlarge	5 reviewed by: Verified Customer	ICC	Mouse	10/09/2019	View Summary Application Immunocytochemistry Sample Tested testis cells Species Mouse Lot 42590
	4 reviewed by: Verified Customer	IHC-Fr	Other	12/12/2014	View Summary Application Immunohistochemistry-Frozen Sample Tested See PMID 23942865 Species Other
	5 reviewed by: Manuela Buonanno	IHC-Fr	Other	11/07/2013	View Summary Application Immunohistochemistry-Frozen Sample Tested whole C. elegans nematode Species Other
Enlarge	5 reviewed by: Western Forte	WB	Human	06/20/2012	View Summary Application Western Blot Sample Tested Hela whole cell lysate Species Human

Array NB100-148

• Rad51 Antibodies
• Rad51 Lysates
• Rad51 Protein