Name: Rad50 Antibody (13B3)
Brand: Novus Biologicals
SKU: NB100-147
Rating: 4 (1 reviews)

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, Rt, PmSpecies Glossary
Applications	WB, Func, ICC/IF, IHC, IP, KD
Clone	13B3
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated

Immunogen	Amino acids 1-425 of Rad50 expressed in E. coli.
Localization	Nuclear
Specificity	This is specific for Rad50.
Isotype	IgG1
Clonality	Monoclonal
Host	Mouse
Gene	RAD50
Purity	Protein G purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	PBS
Preservative	No Preservative
Purity	Protein G purified

Publications using NB100-147	Applications	Species
Sivalingam VN, Latif A, Kitson S et al. Hypoxia and hyperglycaemia determine why some endometrial tumours fail to respond to metformin Br. J. Cancer 2019-12-10 [PMID: 31819173] (IHC-P, Human)	IHC-P	Human
Marie-Egyptienne DT, Chaudary N, Kalliomaki T et al. Cancer initiating-cells are enriched in the CA9 positive fraction of primary cervix cancer xenografts. Oncotarget. 2017-01-03 [PMID: 27901496] (IHC-P, Mouse)	IHC-P	Mouse
Semba H, Takeda N, Isagawa T et al. HIF-1alpha-PDk1 axis-induced active glycolysis plays an essential role in macrophage migratory capacity. Nat Commun. 2016-05-18 [PMID: 27189088] (IF/IHC, Mouse)	IF/IHC	Mouse
Samba-Louaka A, Pereira Jm, Nahori Ma et al. Listeria monocytogenes Dampens the DnA Damage Response. PLoS Pathog. 2014-10-01 [PMID: 25340842] (ICC/IF, Human)	ICC/IF	Human
Khongkow P, Karunarathna U, Khongkow M et al. FOXM1 targets NBS1 to regulate DNA damage-induced senescence and epirubicin resistance. Oncogene. 2013-10-21 [PMID: 24141789] (WB, Mouse, Human)	WB	Mouse, Human
Masuda Y, Noguchi K, Segawa H et al. Novel regulatory role for Kaposi's sarcoma-associated herpesvirus-encoded vFLIP in chemosensitization to bleomycin. Biochem Biophys Res Commun 2011-10-08 [PMID: 22037577] (Human)		Human
Cheng Q, Barboule N, Frit P et al. ku counteracts mobilization of PARP1 and MRN in chromatin damaged with DNA double-strand breaks. Nucleic Acids Res 2011-12-01 [PMID: 21880593] (Human)		Human
Soares D, Battistella A, Rocca C et al. Ataxia telangiectasia mutated- and Rad3-related kinase drives both the early and the late DNA-damage response to the monofunctional antitumour alkylator S23906 Biochem J 2011-07-01 [PMID: 21470188] (WB, Human)	WB	Human
Baydoun HH, Pancewicz J, Nicot C. Human T-lymphotropic type 1 virus p30 inhibits homologous recombination and favors unfaithful DNA repair. Blood 2011-06-01 [PMID: 21427292] (Human)		Human
Chen H, Ma Z et al. The mTOR inhibitor rapamycin suppresses DNA double-strand break repair. Radiat Res 2011-01-02 [PMID: 21268715] (WB, Human)	WB	Human
Stoepker C, Hain K, Schuster B et al. SLX4, a coordinator of structure-specific endonucleases, is mutated in a new Fanconi anemia subtype. Nat Genet 2011-02-01 [PMID: 21240277] (Human)		Human
Anderson VE, Walton MI, Eve PD et al. CCT241533 is a potent and selective inhibitor of CHK2 that potentiates the cytotoxicity of PARP inhibitors. Cancer Res 2011-01-01 [PMID: 21239475] (Human)		Human
Matsumoto Y, Miyamoto T, Sakamoto H et al. Two unrelated patients with MRE11A mutations and Nijmegen breakage syndrome-like severe microcephaly. DNA Repair (Amst) 2011-03-01 [PMID: 21227757] (Human)		Human
Luo Y, Chen AY, Qiu J et al. Bocavirus infection induces a DNA damage response that facilitates viral DNA replication and mediates cell death. J Virol 2011-01-01 [PMID: 21047968] (Human)		Human
Kuroda S, Fujiwara T, Shirakawa Y et al. Telomerase-dependent oncolytic adenovirus sensitizes human cancer cells to ionizing radiation via inhibition of DNA repair machinery. Cancer Res 2010-11-01 [PMID: 21045143] (Human)		Human
Machida K, McNamara G, Cheng KT et al. Hepatitis C virus inhibits DNA damage repair through reactive oxygen and nitrogen species and by interfering with the ATM-NBS1/Mre11/Rad50 DNA repair pathway in monocytes and hepatocytes. J Immunol 2010-12-01 [PMID: 20974981] (Human)		Human
Balasubramanian N, Bai P, Buchek G et al. Physical interaction between the herpes simplex virus type 1 exonuclease, UL12, and the DNA double-strand break-sensing MRN complex. J Virol 2010-12-01 [PMID: 20943970] (Human)		Human
Gomez-Godinez V, Wu T, Sherman AJ et al. Analysis of DNA double-strand break response and chromatin structure in mitosis using laser microirradiation. Nucleic Acids Res 2010-12-01 [PMID: 20923785] (Human)		Human
Palagyi A, Neveling K, Plinninger U et al. Genetic inactivation of the Fanconi anemia gene FANCC identified in the hepatocellular carcinoma cell line HuH-7 confers sensitivity towards DNA-interstrand crosslinking agents. Mol Cancer 2010-05-01 [PMID: 20509860] (Human)		Human
Cha H, Lowe JM, Li H et al. Wip1 directly dephosphorylates gamma-H2AX and attenuates the DNA damage response. Cancer Res 2010-05-01 [PMID: 20460517] (Human)		Human
Lee JH, Goodarzi AA, Jeggo PA et al. 53BP1 promotes ATM activity through direct interactions with the MRN complex. EMBO J 2010-02-01 [PMID: 20010693] (Human)		Human
Collaco RF, Bevington JM, Bhrigu V et al. Adeno-associated virus and adenovirus coinfection induces a cellular DNA damage and repair response via redundant phosphatidylinositol 3-like kinase pathways. Virology 2009-09-01 [PMID: 19628243] (Human)		Human
Abuzeid W M, Jiang X et al. Molecular disruption of RAD50 sensitizes human tumor cells to cisplatin-based chemotherapy. J Clin Invest 2009-01-07 [PMID: 19487811] (WB, Human)	WB	Human
Waltes R, Kalb R, Gatei M et al. Human RAD50 deficiency in a Nijmegen breakage syndrome-like disorder. Am J Hum Genet. 2009-05-01 [PMID: 19409520] (Human)		Human
Bartkova J, Tommiska J, Oplustilova L et al. Aberrations of the MRE11-RAD50-NBS1 DNA damage sensor complex in human breast cancer: MRE11 as a candidate familial cancer-predisposing gene. Mol Oncol 2008-12-01 [PMID: 19383352] (Human)		Human
Schwartz RA, Carson CT, Schuberth C et al. Adeno-associated virus replication induces a DNA damage response coordinated by DNA-dependent protein kinase. J Virol 2009-06-01 [PMID: 19339345] (Human)		Human
Zheng L, Kanagaraj R, Mihaljevic B et al. MRE11 complex links RECQ5 helicase to sites of DNA damage. Nucleic Acids Res 2009-05-01 [PMID: 19270065] (Human)		Human
Karen KA, Hoey PJ, Young CSH et al. Temporal regulation of the Mre11-Rad50-Nbs1 complex during adenovirus infection. J Virol;83(9):4565-73. 2009-05-01 [PMID: 19244322]
Carson CT, Orazio NI, Lee DV et al. Mislocalization of the MRN complex prevents ATR signaling during adenovirus infection. EMBO J 2009-03-01 [PMID: 19197236] (Human)		Human
Lakdawala SS, Schwartz RA, Ferenchak K et al. Differential requirements of the C terminus of Nbs1 in suppressing adenovirus DNA replication and promoting concatemer formation. J Virol 2008-09-01 [PMID: 18562516] (Human)		Human
Cervelli T, Palacios JA, Zentilin L et al. Processing of recombinant AAV genomes occurs in specific nuclear structures that overlap with foci of DNA-damage-response proteins. J Cell Sci 2008-02-01 [PMID: 18216333] (Human)		Human
Gregory DA, Bachenheimer SL. Characterization of mre11 loss following HSV-1 infection. Virology 2008-03-01 [PMID: 18177684] (Human)		Human
Schwartz RA, Palacios JA, Cassell GD et al. The Mre11/Rad50/Nbs1 complex limits adeno-associated virus transduction and replication. J Virol 2007-12-01 [PMID: 17898048] (Human)		Human
Gorodetsky E, Calkins S, Ahn J, Brooks PJ ATM, the Mre11/Rad50/Nbs1 complex, and topoisomerase I are concentrated in the nucleus of Purkinje neurons in the juvenile human brain. DNA Repair (Amst) 2007-11-01 [PMID: 17706468]
Zhong ZH, Jiang WQ, Cesare AJ et al. Disruption of telomere maintenance by depletion of the MRE11/RAD50/NBS1 complex in cells that use alternative lengthening of telomeres. J Biol Chem 2007-10-01 [PMID: 17693401] (Human)		Human
Bentle MS, Reinicke KE, Dong Y et al. Nonhomologous end joining is essential for cellular resistance to the novel antitumor agent, beta-lapachone. Cancer Res 2007-07-01 [PMID: 17638905] (Human)		Human
Cho YW, Hong T, Hong S et al. PTIP associates with MLL3- and MLL4-containing histone H3 lysine 4 methyltransferase complex. J Biol Chem 2007-07-01 [PMID: 17500065] (Human)		Human
Bae NS, Baumann P. A RAP1/TRF2 complex inhibits nonhomologous end-joining at human telomeric DNA ends. Mol Cell 2007-05-01 [PMID: 17499040] (Human)		Human
Lin X, Liu CC, Gao Q et al. RINT-1 serves as a tumor suppressor and maintains Golgi dynamics and centrosome integrity for cell survival. Mol Cell Biol 2007-07-01 [PMID: 17470549] (Human)		Human
You KT, Li LS, Kim NG et al. Selective translational repression of truncated proteins from frameshift mutation-derived mRNAs in tumors. PLoS Biol 2007-05-01 [PMID: 17456004] (Human)		Human
Dote H, Burgan WE, Camphausen K et al. Inhibition of hsp90 compromises the DNA damage response to radiation. Cancer Res 2006-09-01 [PMID: 16982765] (Human)		Human
Takemura H, Rao VA, Sordet O et al. Defective Mre11-dependent activation of Chk2 by ataxia telangiectasia mutated in colorectal carcinoma cells in response to replication-dependent DNA double strand breaks. J Biol Chem 2006-10-01 [PMID: 16905549] (Human)		Human
Kong LJ, Meloni AR, Nevins JR. The Rb-related p130 protein controls telomere lengthening through an interaction with a Rad50-interacting protein, RINT-1. Mol Cell 2006-04-01 [PMID: 16600870] (Human)		Human
Myers JS, Cortez D. Rapid activation of ATR by ionizing radiation requires ATM and Mre11. J Biol Chem 2006-04-01 [PMID: 16431910] (Human)		Human
Tommiska J, Seal S, Renwick A et al. Evaluation of RAD50 in familial breast cancer predisposition. Int J Cancer 2006-06-01 [PMID: 16385572] (Human)		Human
Al Rashid, S et al. Evidence for the Direct Binding of Phosphorylated p53 to Sites of DNA Breaks In vivo. Cancer Res 65(23): 10810-10821. 2005-01-01 [PMID: 16322227]
Mongiat-Artus P, Miquel C, Van der Aa M et al. Microsatellite instability and mutation analysis of candidate genes in urothelial cell carcinomas of upper urinary tract. Oncogene 2006-03-01 [PMID: 16288216] (Human)		Human
Oishi H, Kitagawa H, Wada O et al. An hGCN5/TRRAP histone acetyltransferase complex co-activates BRCA1 transactivation function through histone modification. J Biol Chem 2006-01-01 [PMID: 16260778] (Human)		Human
Liu Y, Shevchenko A, Shevchenko A et al. Adenovirus exploits the cellular aggresome response to accelerate inactivation of the MRN complex. J Virol 2005-11-01 [PMID: 16254336] (Human)		Human
Kim JS, Krasieva TB, Kurumizaka H et al. Independent and sequential recruitment of NHEJ and HR factors to DNA damage sites in mammalian cells. J Cell Biol 2005-08-01 [PMID: 16061690] (Human)		Human
Koh KH, Kang HJ, Li LS et al. Impaired nonhomologous end-joining in mismatch repair-deficient colon carcinomas. Lab Invest 2005-09-01 [PMID: 16025146] (Human)		Human
van Veelen LR, Cervelli T, van de Rakt MW et al. Analysis of ionizing radiation-induced foci of DNA damage repair proteins. Mutat Res 2005-07-01 [PMID: 15914204] (Human)		Human
Lilley CE, Carson CT, Muotri AR et al. DNA repair proteins affect the lifecycle of herpes simplex virus 1. Proc Natl Acad Sci USA 2005-04-01 [PMID: 15824307] (Human)		Human
Fasching CL, Bower K, Reddel RR. Telomerase-independent telomere length maintenance in the absence of alternative lengthening of telomeres-associated promyelocytic leukemia bodies. Cancer Res 2005-04-01 [PMID: 15805271] (Human)		Human
Angele S, Jones C, Reis Filho JS et al. Expression of ATM, p53, and the MRE11-Rad50-NBS1 complex in myoepithelial cells from benign and malignant proliferations of the breast. J Clin Pathol 2004-11-01 [PMID: 15509680] (Human)		Human
Wu, X et al. SV40 T antigen interacts with Nbs1 to disrupt DNA replication control. Genes; Development 18: 1305-1316. 2004-01-01 [PMID: 15175262]
O'Connor MS, Safari A, Liu D et al. The human Rap1 protein complex and modulation of telomere length. J Biol Chem 2004-07-01 [PMID: 15100233] (Human)		Human
Wilkinson DE, Weller SK. Recruitment of cellular recombination and repair proteins to sites of herpes simplex virus type 1 DNA replication is dependent on the composition of viral proteins within prereplicative sites and correlates with the induction of the DNA damage response. J Virol 2004-05-01 [PMID: 15078960] (Human)		Human
Dork T, Bendix-Waltes R, Wegner RD et al. Slow progression of ataxia-telangiectasia with double missense and in frame splice mutations. Am J Med Genet A 2004-04-01 [PMID: 15054841] (Human)		Human
Giannini G, Rinaldi C, Ristori E et al. Mutations of an intronic repeat induce impaired MRE11 expression in primary human cancer with microsatellite instability. Oncogene 2004-04-01 [PMID: 15048091] (Human)		Human
Carson CT, Schwartz RA, Stracker TH et al. The Mre11 complex is required for ATM activation and the G2/M checkpoint. EMBO J 2003-12-01 [PMID: 14657032] (Human)		Human
Uziel T, Lerenthal Y, Moyal L et al. Requirement of the MRN complex for ATM activation by DNA damage. EMBO J 2003-10-01 [PMID: 14532133] (Human)		Human
Xu X, Stern DF. NFBD1/MDC1 regulates ionizing radiation-induced focus formation by DNA checkpoint signaling and repair factors. FASEB J 2003-10-01 [PMID: 14519663] (Human)		Human
Angele S, Treilleux I, Bremond A et al. Altered expression of DNA double-strand break detection and repair proteins in breast carcinomas. Histopathology 2003-10-01 [PMID: 14511253] (Human)		Human
Evans, J. Distinct Roles of the Adenovirus E4 ORF3 Protein in Viral DNA Replication Inhibition of Genome Concatenation. J Virol 77(9): 5295-5304. 2003-01-01 [PMID: 12692231]
Stewart GS, Wang B, Bignell CR et al. MDC1 is a mediator of the mammalian DNA damage checkpoint. Nature 2003-02-01 [PMID: 12607005] (Human)		Human
Anderson LA, Perkins ND. Regulation of RelA (p65) function by the large subunit of replication factor C. Mol Cell Biol 2003-01-01 [PMID: 12509469] (Human)		Human
Hassane DC, Lee RB, Pickett CL. Campylobacter jejuni cytolethal distending toxin promotes DNA repair responses in normal human cells. Infect Immun 2003-01-01 [PMID: 12496208] (Human)		Human
Richie CT, Peterson C, Lu T et al. hSnm1 colocalizes and physically associates with 53BP1 before and after DNA damage. Mol Cell Biol 2002-12-01 [PMID: 12446782] (Human)		Human
Pichierri P, Averbeck D, Rosselli F et al. DNA cross-link-dependent RAD50/MRE11/NBS1 subnuclear assembly requires the Fanconi anemia C protein. Hum Mol Genet 2002-10-01 [PMID: 12354779] (Human)		Human
Kim JS, Krasieva TB, LaMorte V et al. Specific recruitment of human cohesin to laser-induced DNA damage. J Biol Chem 2002-11-01 [PMID: 12228239] (Human)		Human
Stracker TH, Carson CT, Weitzman MD. Adenovirus oncoproteins inactivate the Mre11-Rad50-NBS1 DNA repair complex. Nature 2002-07-01 [PMID: 12124628] (Human)		Human
Franchitto A, Pichierri P. Bloom's syndrome protein is required for correct relocalization of RAD50/MRE11/NBS1 complex after replication fork arrest. J Cell Biol 2002-04-01 [PMID: 11916980] (Human)		Human
Carbone, R et al. PML NBs associate with the hMre11 complex p53 at sites of irradiation induced DNA damage. Oncogene 21: 1633-1640. 2002-01-01 [PMID: 11896594]
Harfst E, Cooper S, Neubauer S et al. Normal V(D)J recombination in cells from patients with Nijmegen breakage syndrome. Mol Immunol 2000-10-01 [PMID: 11282395] (Human)		Human
Tauchi H, Kobayashi J, Morishima K et al. The forkhead-associated domain of NBS1 is essential for nuclear foci formation after irradiation but not essential for hRAD50[middle dot]hMRE11[middle dot]NBS1 complex DNA repair activity. J Biol Chem 2001-01-01 [PMID: 11062235] (Human)		Human
Paull TT, Rogakou EP, Yamazaki V et al. A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage. Curr Biol 2000-07-01 [PMID: 10959836] (Human)		Human
Wang Y, Cortez D, Yazdi P et al. BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures. Genes Dev 2000-04-01 [PMID: 10783165] (Human)		Human
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Rad50 Antibody (13B3)

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	Anatoli Grechov 12/17/2014
Application:	WB
Species:	Human

Gene Symbol	RAD50
Entrez	10111(Human)