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APLF Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: APLF Antibody [NBP2-38212] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: APLF Antibody [NBP2-38212] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells and glial cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

APLF Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: SQLEGSTEIAKTQMTPTNSVSFLGENRDCNKQQPILAERKRILPTWMLAEHLSDQNLSVPAISGGNVIQGSGKEEICKDKSQLN
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
APLF
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
APLF Protein (NBP2-38212PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for APLF Antibody

  • APFL
  • aprataxin- and PNK-like factor
  • aprataxin and PNK-like factor
  • aprataxin and PNKP like factor
  • Apurinic-apyrimidinic endonuclease APLF
  • C2orf13FLJ16593
  • chromosome 2 open reading frame 13
  • EC 4.2.99.18
  • MGC47799
  • PALF
  • PNK and APTX-like FHA domain-containing protein
  • PNK and APTX-like FHA protein
  • Xip1
  • XRCC1-interacting protein 1

Background

C2ORF13 is a component of the cellular response to chromosomal DNA single- and double-strand breaks (Iles et al., 2007(PubMed 17353262)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol APLF
Uniprot