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DNA-PKcs Antibody (3H6) - BSA Free

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Western Blot: DNA-PKcs Antibody (3H6) [NBP2-22128] - HeLa (A), MCF7 (lB) and HEK293 (C) whole cell protein was separated by SDS-PAGE on 6% gel, transferred to PVDF and probed with NBP2-22128. A single band of DNA PKcs reactivity at approximately 460 kDa was detected in all lysates.
Western Blot: DNA-PKcs Antibody (3H6) [NBP2-22128] - HeLa (A), MCF7 (lB) and HEK293 (C) whole cell protein was separated by SDS-PAGE on 6% gel, transferred to PVDF and probed with NBP2-22128. A single band of DNA PKcs ...read more
Immunocytochemistry/ Immunofluorescence: DNA-PKcs Antibody (3H6) [NBP2-22128] - Analysis of Hela cells using DNA PKcs mouse mAb (green). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).
Immunohistochemistry-Paraffin: DNA-PKcs Antibody (3H6) [NBP2-22128] - Analysis of paraffin-embedded lung cancer (left) and brain tissues (right) using DNA PKcs mouse mAb with DAB staining.
Western Blot: DNA-PKcs Antibody (3H6) [NBP2-22128] - Analysis using DNA PKcs mAb against HEK293 (1) and DNA PKcs(AA: 2638-2971)-hIgGFc transfected HEK293 (2) cell lysates.
Immunohistochemistry-Paraffin: DNA-PKcs Antibody (3H6) [NBP2-22128] - Analysis of paraffin-embedded breast cancer (left) and colon cancer (right) using DNA PKcs mouse mAb with DAB staining.
ELISA: DNA-PKcs Antibody (3H6) [NBP2-22128] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
3H6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-22128AF350
Alexa Fluor 405
NBP2-22128AF405
Alexa Fluor 488
NBP2-22128AF488
Alexa Fluor 532
NBP2-22128AF532
Alexa Fluor 594
NBP2-22128AF594
Alexa Fluor 647
NBP2-22128AF647
Alexa Fluor 700
NBP2-22128AF700
Alexa Fluor 750
NBP2-22128AF750
Allophycocyanin
NBP2-22128APC
Biotin
NBP2-22128B
CoraFluor 1
NBP2-22128CL1
DyLight 350
NBP2-22128UV
DyLight 405
NBP2-22128V
DyLight 488
NBP2-22128G
DyLight 550
NBP2-22128R
DyLight 594
NBP2-22128DL594
DyLight 650
NBP2-22128C
DyLight 680
NBP2-22128FR
DyLight 755
NBP2-22128IR
FITC
NBP2-22128F
HRP
NBP2-22128H
Janelia Fluor 525
NBP2-22128JF525
Janelia Fluor 549
NBP2-22128JF549
Janelia Fluor 646
NBP2-22128JF646
Janelia Fluor 669
NBP2-22128JF669
PE
NBP2-22128PE
PerCP
NBP2-22128PCP
mFluor Violet 450 SE
NBP2-22128MFV450
mFluor Violet 500 SE
NBP2-22128MFV500
mFluor Violet 610 SE
NBP2-22128MFV610
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-22128SS
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DNA-PKcs Antibody (3H6) - BSA Free Summary

Immunogen
Purified recombinant fragment of human DNA PKcs expressed in E. coli. [Uniprot: P78527]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PRKDC
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
Application Notes
In Western blot, a band can be seen at 460-470 kDa representing DNA PKcs.
Theoretical MW
460 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-22128 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for DNA-PKcs Antibody (3H6) - BSA Free

  • DNA-dependent protein kinase catalytic subunit-like
  • DNA-dependent protein kinase
  • DNA-PK catalytic subunit
  • DNAPK
  • DNAPKcs
  • DNA-PKcs
  • DNPK1
  • EC 2.7.11.1
  • HYRC
  • HYRC1
  • p350
  • p460
  • PRKDC
  • XRCC7

Background

The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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DNA-PKcs Antibody
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NBP2-22128
DNA-PKcs Antibody (3H6) - BSA Free
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC

Publications for DNA-PKcs Antibody (NBP2-22128)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


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Bioinformatics

Gene Symbol PRKDC
Entrez
Uniprot