![Western Blot: XRCC4 Antibody (4H9) [NBP1-48053] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC4.](http://images.novusbio.com/fullsize/XRCC4-Antibody-4H9-Western-Blot-NBP1-48053-img0001.jpg "Western Blot: XRCC4 Antibody (4H9) [NBP1-48053] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC4.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clone | OTI4H9 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full-length protein expressed in 293T cell transfected with human XRCC4 expression vector |
| Specificity | This antibody is specific for Homo sapiens X-ray repair complementing defective repair in Chinese hamster cells 4 (XRCC4), transcript variant 3. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | XRCC4 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 38.3 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP1-48053 | Applications | Species |
|---|---|---|
| Xing M, Oksenych V Genetic interaction between DNA repair factors PAXX, XLF, XRCC4 and DNA-PKcs in human cells FEBS Open Bio 2019-05-29 [PMID: 31141305] |
Secondary Antibodies |
Isotype Controls |
Research Areas for XRCC4 Antibody (NBP1-48053)Find related products by research area.
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Ku70: The DNA's Mr. Fix-it Ku70, known by several synonyms including X-ray repair cross-complementing, 5'-deoxyribose-5-phosphate lyase Ku70 protein 6, 70 kDa subunit of Ku antigen, XRCC6, and G22P1, is a 70 kDa protein that was shown to be involved in multiple cellular pathway... Read full blog post. |
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![Immunohistochemistry-Paraffin: XRCC4 Antibody (4H9) [NBP1-48053] Staining of paraffin-embedded thyroid tissue using anti-XRCC4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XRCC4-Antibody-4H9-Immunohistochemistry-Paraffin-NBP1-48053-img0013.jpg "Immunohistochemistry-Paraffin: XRCC4 Antibody (4H9) [NBP1-48053] Staining of paraffin-embedded thyroid tissue using anti-XRCC4 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: XRCC4 Antibody (4H9) [NBP1-48053] - Staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-XRCC4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XRCC4-Antibody-4H9-Immunohistochemistry-Paraffin-NBP1-48053-img0004.jpg "Immunohistochemistry-Paraffin: XRCC4 Antibody (4H9) [NBP1-48053] - Staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-XRCC4 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: XRCC4 Antibody (4H9) [NBP1-48053] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-XRCC4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XRCC4-Antibody-4H9-Immunohistochemistry-Paraffin-NBP1-48053-img0005.jpg "Immunohistochemistry-Paraffin: XRCC4 Antibody (4H9) [NBP1-48053] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-XRCC4 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: XRCC4 Antibody (4H9) [NBP1-48053] - Staining of paraffin-embedded colon tissue using anti-XRCC4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XRCC4-Antibody-4H9-Immunohistochemistry-Paraffin-NBP1-48053-img0008.jpg "Immunohistochemistry-Paraffin: XRCC4 Antibody (4H9) [NBP1-48053] - Staining of paraffin-embedded colon tissue using anti-XRCC4 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: XRCC4 Antibody (4H9) [NBP1-48053] - Staining of paraffin-embedded Kidney tissue using anti-XRCC4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XRCC4-Antibody-4H9-Immunohistochemistry-Paraffin-NBP1-48053-img0010.jpg "Immunohistochemistry-Paraffin: XRCC4 Antibody (4H9) [NBP1-48053] - Staining of paraffin-embedded Kidney tissue using anti-XRCC4 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: XRCC4 Antibody (4H9) [NBP1-48053] - Staining of paraffin-embedded lung tissue using anti-XRCC4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/XRCC4-Antibody-4H9-Immunohistochemistry-Paraffin-NBP1-48053-img0011.jpg "Immunohistochemistry-Paraffin: XRCC4 Antibody (4H9) [NBP1-48053] - Staining of paraffin-embedded lung tissue using anti-XRCC4 mouse monoclonal antibody.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")