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Orexin Antibody

Images

 
Western Blot: Orexin Antibody [NBP3-12327] - 1:500 antibody dilution in DiluObuffer. Apparent MW is 16 KDa.
Immunohistochemistry-Paraffin: Orexin Antibody [NBP3-12327] - Rat Spinal Cord. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin (QS) blue counterstain. 40X maginfication.
Immunohistochemistry-Paraffin: Orexin Antibody [NBP3-12327] - Mouse Brain. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin (QS) blue counterstain. 5X maginfication.
Immunohistochemistry-Paraffin: Orexin Antibody [NBP3-12327] - Mouse Brain. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin (QS) blue counterstain. 40X maginfication.

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, ELISA, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Orexin Antibody Summary

Immunogen
Synthetic peptide taken within amino acid region 1-50 on ORXN protein.
Clonality
Polyclonal
Host
Rabbit
Gene
HCRT
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:200
  • Western Blot 1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Contains Tris, HCl/glycine buffer (pH 7.4-7.8), 30% glycerol, 0.5% BSA, along with cryo-protective agents, and HEPES (exact storage and stabilization buffer information is proprietary).
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Orexin Antibody

  • hypocretin (orexin) neuropeptide precursor
  • NRCLP1
  • orexin
  • OXHcrt
  • PPORX
  • PPOXHypocretin

Background

Orexin, or hypocretin (HCRT), is a hypothalamic neuropeptide precusor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, are mediators in the central feedback mechanism that regulates feeding behavior.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Orexin Products

Array NBP3-12327

Research Areas for Orexin Antibody (NBP3-12327)

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Blogs on Orexin

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Bioinformatics

Gene Symbol HCRT