![Immunohistochemistry: Neuropeptide Y Antibody [NBP1-46535] - Low magnification images showing the double staining pattern of NHE1 with neuropeptide NPY. Scale bar: 100um. Insets: High magnification images showing individual cells with double staining. Scale bar: 10um. Asterisks mark Hoechst-stained nuclei. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-019-42872-w), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Neuropeptide-Y-Antibody-Immunohistochemistry-NBP1-46535-img0001.jpg "Immunohistochemistry: Neuropeptide Y Antibody [NBP1-46535] - Low magnification images showing the double staining pattern of NHE1 with neuropeptide NPY. Scale bar: 100um. Insets: High magnification images showing individual cells with double staining. Scale bar: 10um. Asterisks mark Hoechst-stained nuclei. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-019-42872-w), licensed under a CC-BY license.")
| Reactivity | Hu, Mu, Rt, Rb, XpSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Immunogen | A synthetic peptide from amino acid region 20-70 of mouse Neuropeptide Y conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in many other species including human, rat, zebrafish and xenopus. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | NPY |
| Purity | Unpurified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Use in ICC/IF reported in scientific literature (PMID: 27612517). Use in IHC-FrFl reported in scientific literature (PMID: 31609204). |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from whole antisera |
| Preservative | No Preservative |
| Purity | Unpurified |
| Reconstitution Instructions | Reconstitute in 150 ul of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format. |
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/ Immunofluorescence: Neuropeptide Y Antibody [NBP1-46535] - NHE1 distribution (A) & colocalisation with markers for specific cell types (B–D) & major inputs (E, F, G). (A) NHE1 immunoreactivity is distributed throughout the rostrocaudal axis of the SCN (encircled by the dotted lines). Scale bar: 200 µm. OC: optic chiasm. 3 V: third ventricle. (B–G1) Low magnification images showing the double staining pattern of NHE1 with neuropeptides NP2 (B), GRP (C), & VIP (D) as well as markers for afferent inputs vGluT2 (E), NPY (F), & SERT (G1). Scale bar: 100 µm. Insets: High magnification images showing individual cells with double staining. Scale bar: 10 µm. Asterisks mark Hoechst-stained nuclei. (G2) High magnification image showing high degree of colocalisation (yellow) between NHE1 (green) & SERT (red). Scale bar: 10 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31015514), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-46535_goat-polyclonal-neuropeptide-y-antibody-310202416161848.jpg "Immunocytochemistry/ Immunofluorescence: Neuropeptide Y Antibody [NBP1-46535] - NHE1 distribution (A) & colocalisation with markers for specific cell types (B–D) & major inputs (E, F, G). (A) NHE1 immunoreactivity is distributed throughout the rostrocaudal axis of the SCN (encircled by the dotted lines). Scale bar: 200 µm. OC: optic chiasm. 3 V: third ventricle. (B–G1) Low magnification images showing the double staining pattern of NHE1 with neuropeptides NP2 (B), GRP (C), & VIP (D) as well as markers for afferent inputs vGluT2 (E), NPY (F), & SERT (G1). Scale bar: 100 µm. Insets: High magnification images showing individual cells with double staining. Scale bar: 10 µm. Asterisks mark Hoechst-stained nuclei. (G2) High magnification image showing high degree of colocalisation (yellow) between NHE1 (green) & SERT (red). Scale bar: 10 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31015514), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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