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FIH-1/HIF-1AN Antibody [DyLight 488]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP, MS, KD, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 488

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-428AF350
Alexa Fluor 405
NB100-428AF405
Alexa Fluor 488
NB100-428AF488
Alexa Fluor 532
NB100-428AF532
Alexa Fluor 594
NB100-428AF594
Alexa Fluor 647
NB100-428AF647
Alexa Fluor 700
NB100-428AF700
Alexa Fluor 750
NB100-428AF750
Allophycocyanin
NB100-428APC
Biotin
NB100-428B
DyLight 350
NB100-428UV
DyLight 405
NB100-428V
DyLight 550
NB100-428R
DyLight 594
NB100-428DL594
DyLight 650
NB100-428C
DyLight 680
NB100-428FR
DyLight 755
NB100-428IR
FITC
NB100-428F
HRP
NB100-428H
Janelia Fluor 549
NB100-428JF549
Janelia Fluor 646
NB100-428JF646
PE
NB100-428PE
PerCP
NB100-428PCP
Unconjugated
NB100-428
mFluor Violet 450 SE
NB100-428MFV450
mFluor Violet 500 SE
NB100-428MFV500
mFluor Violet 610 SE
NB100-428MFV610
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FIH-1/HIF-1AN Antibody [DyLight 488] Summary

Immunogen
A synthetic peptide made to the C-terminal region of human Factor Inhibiting HIF-1 (between residues 300 and the C-terminus). [UniProt# Q9NWT6]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIF1AN
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Electron Microscopy
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockdown Validated
  • Knockout Validated
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Human, rat and mouse (PMID 19720742).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for FIH-1/HIF-1AN Antibody [DyLight 488]

  • DKFZp762F1811
  • EC 1.14.11.16
  • factor inhibiting HIF1
  • Factor inhibiting HIF-1
  • FIH1
  • FIH-1
  • FIH1FIH-1
  • FLJ20615
  • FLJ22027
  • HIF1AN
  • HIF-1AN
  • hypoxia inducible factor 1, alpha subunit inhibitor
  • hypoxia-inducible factor 1-alpha inhibitor
  • Hypoxia-inducible factor asparagine hydroxylase
  • Peptide-aspartate beta-dioxygenase

Background

Factor Inhibiting HIF1 (FIH) protein facilitates repression of HIF-1 transcriptional activity by binding to VHL, which acts as a transcriptional corepressor. VHL inhibits HIF-1 alpha transactivation function by recruiting histone deacetylases. Involvement of VHL in association with FIH provides a unifying mechanism for the modulation of HIF-1 alpha protein stabilization and transcriptional activation in response to changes in cellular oxygen concentration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FIH-1/HIF-1AN
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NB100-428G
FIH-1/HIF-1AN Antibody [DyLight 488]
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IP, MS, KD, KO

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Bioinformatics

Gene Symbol HIF1AN