FIH-1/HIF-1AN Antibody

Factor inhibiting HIF1 (FIH1) is an asparaginyl hydroxylase enzyme that regulates HIF's transcriptional activity by hydroxylating HIF-1 alpha at 'Asp-803' in the CAD (C-terminal transactivation domain). Hydroxylation via PHDs followed by proteosome degradation is another mechanism that regulates HIF1, however, FIH1 is able to exercise the control even under severe hypoxic conditions, when PHD enzymes fail to do so. Localized mainly in cytoplasm, FIH1 functions as oxygen sensor and FIH1 interaction with NOTCH1 results in its nuclear localization whereas FIH1-ABPA3 interaction leads to its perinuclear localization. During normoxic conditions, FIH1 hydroxylates a conserved asparaginyl residue within CTAD of HIF-1 alpha/HIF-2 alpha, leading to steric clash that prevents the recruitment of the co-activators p300 and CBP. On the other hand, under hypoxic conditions, FIH1 is inactive, which results in activation of HIF-alpha signaling. FIH1 is involved in transcriptional repression through interaction with HIF1A, VHL and HDAC and hydroxylates specific Asn residues within ARD (ankyrin repeat domains) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A as well as other ARD-containing proteins. FIH1 also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. FIH1 has the ability to negatively regulate NOTCH1 activity thus accelerating myogenic differentiation, and positively regulates ASB4 activity which promotes vascular differentiation. FIH1-Mint3 binding affects FIH1's ability to modify HIF-1 alpha in an oxygen-independent manner and FIH1-Bax interaction plays inhibitory role in apoptosis.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: EM, ICC/IF, IHC-P, IP, KO, WB.

Showing Publications 1 - 10 of 29. Show All 29 Publications. Collapse Publications.

Publications using NB100-428	Applications	Species
E G, Sun B, Liu B et al. Enhanced BPGM/2,3-DPG pathway activity suppresses glycolysis in hypoxic astrocytes via FIH-1 and TET2 Brain research bulletin 2022-11-08 [PMID: 36334804] (WB, Human) Details: Dilution used in WB 1:1000	WB	Human
Vera Schützhold, Jan Gravemeyer, Anne Bicker, Thomas Hager, Claudia Padberg, Jana Schäfer, Anna Wrobeln, Melanie Steinbrink, Seher Zeynel, Thomas Hankeln, Jürgen Christian Becker, Joachim Fandrey, Sandra Winning Knockout of Factor-Inhibiting HIF ( Hif1an ) in Colon Epithelium Attenuates Chronic Colitis but Does Not Reduce Colorectal Cancer in Mice. Journal of immunology (Baltimore, Md. : 1950) 2022-03-07 [PMID: 35121641]
Li ZL, Wang B, Lv LL et al. FIH-1-modulated HIF-1 alpha C-TAD promotes acute kidney injury to chronic kidney disease progression via regulating KLF5 signaling Acta pharmacologica Sinica 2021-03-03 [PMID: 33658705]
Chung Y, Saitoh Y, Hayashi T et al. Mint3 is dispensable for pancreatic and kidney functions in mice Biochemistry and biophysics reports 2020-12-01 [PMID: 33319072] (WB)	WB
Mader J, Huber J, Bonn F et al. Oxygen-dependent asparagine hydroxylation of the ubiquitin-associated (UBA) domain in Cezanne regulates ubiquitin binding J. Biol. Chem. 2020-01-14 [PMID: 31937588] (WB, KO, Human)	WB, KO	Human
Ugidos N, Mena J, Baquero S et al. Interactome of the Autoimmune Risk Protein ANKRD55 Front. Immunol. 2019-09-18 [PMID: 31620119]
Docherty C K, Nilsen M et al. Influence of 2-Methoxyestradiol and Sex on Hypoxia-Induced Pulmonary Hypertension and Hypoxia-Inducible Factor-1-alpha. J Am Heart Assoc 2019-05-03 [PMID: 30819028] (WB, Human)	WB	Human
Pillai MR, Mihi B, Ishiwata K et al. Myc-induced nuclear antigen constrains a latent intestinal epithelial cell-intrinsic anthelmintic pathway PLoS ONE 2019-02-26 [PMID: 30807587] (WB, Mouse)	WB	Mouse
Meehan J, Ward C, Turnbull A et al. Inhibition of pH regulation as a therapeutic strategy in hypoxic human breast cancer cells. Oncotarget 2017-04-17 [PMID: 28476026]
Bullen JW, Tchernyshyov I, Holewinski RJ et al. Protein kinase A-dependent phosphorylation stimulates the transcriptional activity of hypoxia-inducible factor 1 Sci Signal 2016-05-31 [PMID: 27245613] (WB, Human)	WB	Human
Nakaoka HJ, Hara T, Yoshino S et al. NECAB3 Promotes Activation of Hypoxia-inducible factor-1 during Normoxia and Enhances Tumourigenicity of Cancer Cells Sci Rep 2016-03-07 [PMID: 26948053] (IP, Human)	IP	Human
Kiriakidis S, Henze AT, Kruszynska-Ziaja I et al. Factor-inhibiting HIF-1 (FIH-1) is required for human vascular endothelial cell survival FASEB J. 2015-04-02 [PMID: 25837583] (WB, Human)	WB	Human
Karttunen S, Duffield M, Scrimgeour Nr et al. Oxygen-dependent hydroxylation by Factor Inhibiting HIF (FIH) regulates the TRPV3 ion channel. J. Cell. Sci. 2014-11-20 [PMID: 25413349] (WB)	WB
Rawluszko AA, Bujnicka KE, Horbacka K et al. Expression and DNA methylation levels of prolyl hydroxylases PHD1, PHD2, PHD3 and asparaginyl hydroxylase FIH in colorectal cancer. BMC Cancer. 2013-11-06 [PMID: 24195777] (WB, Human)	WB	Human
Janke K, Brockmeier U, Kuhlmann K et al. Factor Inhibiting HIF-1 (FIH-1) modulates protein interactions of Apoptosis-Stimulating p53 binding Protein 2 (ASPP2). J Cell Sci 2013-04-19 [PMID: 23606740] (WB, Human)	WB	Human
Nishida C, Kusubata K, Tashiro Y et al. MT1-MMP plays a critical role in hematopoiesis by regulating HIF-mediated chemokine/cytokine gene transcription within niche cells Blood 2012-06-07 [PMID: 22544701]
Lim CS, Kiriakidis S, Paleolog EM, Davies AH. Increased activation of the hypoxia-inducible factor pathway in varicose veins. J Vasc Surg;55(5):1427-1439e1. 2012-05-01 [PMID: 22277691] (WB, Human)	WB	Human
Wilkins SE, Karttunen S, Hampton-Smith RJ et al. Factor inhibiting HIF (FIH) recognises distinct molecular features within hypoxia inducible factor (HIF)-[alpha] versus ankyrin repeat substrates. The Journal of Biological Chemistry. 2012-01-23 [PMID: 22270367]
Melvin A, Mudie S, Rocha S. The chromatin remodeler ISWI regulates the cellular response to hypoxia: role of FIH. Mol Biol Cell 2011-11-01 [PMID: 21900490] (Human)		Human
Singleton RS, Trudgian DC, Fischer R et al. Quantitative mass spectrometry reveals the dynamics of Factor Inhibiting HIF (FIH)-catalysed hydroxylation. J Biol Chem. 2011-07-30 [PMID: 21808058]
Schodel J, Bohr D, Klanke B et al. Factor inhibiting HIF limits the expression of hypoxia-inducible genes in podocytes and distal tubular cells. Kidney Int. 2010-01-01 [PMID: 20720525]
Luo T, Zhang X, Nicholson WL et al. Molecular characterization of antibody epitopes of Ehrlichia chaffeensis ankyrin protein 200 and tandem repeat protein 47 and evaluation of synthetic immunodeterminants for serodiagnosis of human monocytotropic ehrlichiosis. Clin Vaccine Immunol 2010-01-01 [PMID: 19955322]
Minamishima YA, Moslehi J, Padera RF et al. A feedback loop involving the Phd3 prolyl hydroxylase tunes the mammalian hypoxic response in vivo. Mol Cell Biol 2009-11-01 [PMID: 19720742] (WB, Mouse)	WB	Mouse
Chan DA, Kawahara TL, Sutphin PD et al. Tumor vasculature is regulated by PHD2-mediated angiogenesis and bone marrow-derived cell recruitment. Cancer Cell 2009-06-02 [PMID: 19477431]
Xu X, Vaithiyalingam S, Glick GG et al. The basic cleft of RPA70N binds multiple checkpoint proteins, including RAD9, to regulate ATR signaling. Mol Cell Biol 2008-12-01 [PMID: 18936170]
Zheng X, Linke S, Dias JM et al. Interaction with factor inhibiting HIF-1 defines an additional mode of cross-coupling between the Notch and hypoxia signaling pathways. Proc Natl Acad Sci U S A;105(9):3368-73. 2008-03-04 [PMID: 18299578] (WB)	WB
Li, J et al. Protein kinase C-mediated modulation of FIH-1 expression by the homeodomain protein CDP/Cut/Cux. Mol Cell Biol;27(20):7345-53. 2007-10-01 [PMID: 17682059]
Ferguson III, JE, Wu, Y et al. ASB4 is a hydroxylation substrate of FIH and promotes vascular differentiation via an oxygen-dependent mechanism. Mol Cell Biol;27(18):6407-19. 2007-09-01 [PMID: 17636018]
Khan Z, Michalopoulos GK, Stolz DB. Peroxisomal localization of hypoxia-inducible factors and hypoxia-inducible factor regulatory hydroxylases in primary rat hepatocytes exposed to hypoxia-reoxygenation. Am J Pathol 2006-10-01 [PMID: 17003483] (ICC/IF, EM, WB, IHC-P, Rat)	ICC/IF, EM, WB, IHC-P	Rat
Show All 29 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Darren Richard	WB	Rat	06/05/2020	View Summary Application Western Blot Sample Tested Vascular Smooth Muscle Cells,Whole cell lysate Species Rat Lot A3
Enlarge	4 reviewed by: Verified Customer	WB	Human	11/12/2017	View Summary Application Western Blot Sample Tested Cell lysates from prostate cancer cell lines Species Human Lot A2 Comments Comments Sample: Whole cell lysates of prostate cancer cell lines (20ug) Blocking: 5% nonfat dry milk reconstituted in TBS-T for 1 hour at room temperature Primary Antibody diluted (1:500) in 5% nonfat dry milk reconstituted in TBS-T overnight at 4deg C Secondary Antibody: Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution in TBS-T
	4 reviewed by: Verified Customer	WB	Human	12/12/2014	View Summary Application Western Blot Sample Tested See PMID 22277691 Species Human

Array NB100-428

• FIH-1/HIF-1AN Antibodies
• FIH-1/HIF-1AN Lysate
• FIH-1/HIF-1AN Proteins