MDA‑MB‑231 human breast cancer cell line was stained with Mouse Anti-Human MMP‑14/MT1‑MMP Monoclonal Antibody (Catalog # MAB9181, filled histogram) or isotype control antibody (Catalog # MAB0041, open ...read more
MMP‑14/MT1‑MMP was detected in immersion fixed paraffin-embedded sections of Human Prostate Cancer using Mouse Anti-Human MMP‑14/MT1‑MMP Monoclonal Antibody (Catalog # MAB9181) at ...read more
Mouse myeloma cell line NS0-derived recombinant human MMP‑14/MT1‑MMP Tyr112-Ala541 (predicted) Accession # P50281
Specificity
Detects human MMP‑14/MT1‑MMP in direct ELISAs and Western blots. Does not detect E. coli-expressed recombinant human MMP-14 catalytic domain (aa 112-284).
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MMP14
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for MMP-14/MT1-MMP Antibody (128527) [Unconjugated]
EC 3.4.24
EC 3.4.24.80
matrix metallopeptidase 14 (membrane-inserted)
matrix metalloproteinase 14 (membrane-inserted)
matrix metalloproteinase-14
membrane type 1 metalloprotease
Membrane-type matrix metalloproteinase 1
Membrane-type-1 matrix metalloproteinase
MMP14
MMP-14
MMP-X1
MT1MMP
MT1-MMP
MT1-MMPMTMMP1
MT-MMP 1
MT-MMP1
Background
As the first member of membrane type (MT) MMPs, MMP-14, also known as MT1-MMP, plays an important role in extracellular matrix (ECM) remodeling by being able to degrade type I collagen, activate pro-MMP-2 and process cell adhesion molecules such as CD44 and integrin alpha V (1). MMP-14 is therefore a key enzyme in many physiological and pathological processes such as angiogenesis and tumor invasion. Structurally, MMP-14 consists of the following domains: a pro domain containing the furin cleavage site, a catalytic domain containing the zinc-binding site, a hinge region, a hemopexin-like domain, a transmembrane domain, and a cytoplasmic tail (2).
Seike, M. (2003) Cancer Lett. 194:1.
Sato, H. et al. (1994) Nature 370:61.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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