EGLN2/PHD1 Antibody

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

EGLN2 (EGL/EGg Laying-Nine #2; also known as PHD1 and HPH3) is a 43 kDa member of the EglN family of proteins. It is ubiquitously expressed and found principally in the nucleus. EGLN2 hydroxylates proline on HIF-1 alpha . HIF-1 is an alpha / beta heterodimeric transcriptional activator that upregulates genes involved in mitigating the effects of hypoxia. Normally, and in the presence of abundant oxygen, the HIF-1 alpha -chain is hydroxylated by PHD family members, which results in its ubiquitylation and degradation. Under low oxygen tension, EGLN2 activity is decreased, the HIF-1 alpha subunit is retained, and HIF-1 activates genes. Human EGLN2 is 407 amino acids (aa) in length (SwissProt #:Q96KS0). It contains one iron 2-oxoglutarate (Fe2OG) dioxygenase domain (aa 278-376) plus an iron-binding (His297 and His358), and a 2-oxoglutarate-binding (Arg367) site. There is one alternative start site at Met34 that generates a 40 kDa isoform. In addition, there is another potential splice form that shows a 16 aa substitution for aa 1-281. Full-length human EGLN2 shares 91% aa sequence identity with mouse EGLN2.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.

Showing Publications 1 - 7 of 7.

Publications using AF6394	Applications	Species
Dey A, Prabhudesai S, Zhang Y et al. Cystathione ?-synthase regulates HIF-1? stability through persulfidation of PHD2 Science Advances 2020-07-03 [PMID: 32937467]
Dey A, Prabhudesai S, Zhang Y et al. Cystathione ?-synthase regulates HIF-1? stability through persulfidation of PHD2 Science Advances 7/3/2020 [PMID: 32937467]
T Liu, L Zhao, H Hou, L Ding, W Chen, X Li Ginsenoside 20(S)-Rg3 suppresses ovarian cancer migration via hypoxia-inducible factor 1 alpha and nuclear factor-kappa B signals Tumour Biol., 2017-05-01;39(5):1010428317692. 2017-05-01 [PMID: 28459376] (Western Blot, Human)	Western Blot	Human
NQO1 inhibits proteasome-mediated degradation of HIF-1? Nat Commun, 2016-12-14;7(0):13593. 2016-12-14 [PMID: 27966538] (Western Blot, Human)	Western Blot	Human
Eun-Taex Oh Docetaxel induced-JNK2/PHD1 signaling pathway increases degradation of HIF-1? and causes cancer cell death under hypoxia Sci Rep, 2016-06-06;6(0):27382. 2016-06-06 [PMID: 27263528] (Western Blot, Human)	Western Blot	Human
Deschoemaeker S, Di Conza G, Lilla S et al. PHD1 regulates p53-mediated colorectal cancer chemoresistance. EMBO Mol Med. 8/19/2015 [PMID: 26290450]
Liu T, Zhao L, Zhang Y, Chen W, Liu D, Hou H, Ding L, Li X Ginsenoside 20(S)-Rg3 targets HIF-1alpha to block hypoxia-induced epithelial-mesenchymal transition in ovarian cancer cells. PLoS ONE, 2014-09-08;9(9):e103887. 2014-09-08 [PMID: 25197976] (Western Blot, Human)	Western Blot	Human

Array AF6394

• EGLN2/PHD1 Antibodies
• EGLN2/PHD1 Lysates
• EGLN2/PHD1 Proteins