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![Western Blot: FIH-1/HIF-1AN Antibody [NB100-428] - Analysis of astrocytes lysate from Rat brain using FIH-1/HIF-1AN antibody at 1:500 dilution with ECL detection. The antibody detected a major band at ~40 kDa position representing FIH-1/HIF-1AN protein.](http://images.novusbio.com/fullsize/FIH-1-HIF-1AN-Antibody-Western-Blot-NB100-428-img0007.jpg "Western Blot: FIH-1/HIF-1AN Antibody [NB100-428] - Analysis of astrocytes lysate from Rat brain using FIH-1/HIF-1AN antibody at 1:500 dilution with ECL detection. The antibody detected a major band at ~40 kDa position representing FIH-1/HIF-1AN protein.")
![Western Blot: FIH-1/HIF-1AN Antibody [NB100-428] - Representative picture of western blot in histopathologically unchanged tissue (N) and primary cancerous tissue (C) from patients with CRC. Immunodetection of bands was performed with Rp anti- PHD1 (Catalog # NB100-310), - PHD2 (Catalog # NB100-137), - PHD3 (Catalog # NB100-139) and -FIH-1/HIF-1AN Ab (Catalog # NB100-139), followed by incubation with goat anti-rabbit HRP-conjugated Ab. The membrane was stripped and incubated with Rp anti-GAPDH Ab, followed by incubation with goat anti-rabbit HRP-conjugated Ab. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-13-526), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/FIH-1-HIF-1AN-Antibody-Western-Blot-NB100-428-img0010.jpg)
![Immunohistochemistry: FIH-1/HIF-1AN Antibody [NB100-428] - Detection of FIH-1 in rat brain tissue.](http://images.novusbio.com/fullsize/FIH-1-HIF-1AN-Antibody-Immunohistochemistry-NB100-428-img0008.jpg)
![Western Blot: FIH-1/HIF-1AN Antibody [NB100-428] - Rat VSMC (vascular smoot muscle) cells treated (siFIH) or not (CTL) with a specific siRNA against FIH-1. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/FIH-1-HIF-1AN-Antibody-Western-Blot-NB100-428-img0009.jpg)
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Knockdown Validated: FIH-1/HIF-1AN Antibody [NB100-428] - (C) Cytoplasmic FIH-1 (lower band) and MMP14 expression levels in both aerobic and hypoxic conditions in the 3 cell lines. (E) siRNA targeting FIH-1 was used to assess whether FIH-1 had any effect on CAIX expression in MCF-7 cells cultured in 0.5% O2 conditions. cont, control. m, transfection reagent alone. scr, scrambled siRNA. pool, mixture of 4 siRNAs targeting FIH-1. 21, single siRNA targeting FIH-1. (F) FIH-1 and CAIX expression levels in aerobic MCF-7 cells treated with either siRNA against FIH-1 alone, or both 400 uM CoCl2 and siRNA together for 24, 48 and 72h. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28476026/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/FIH-1-HIF-1AN Antibody-Knockdown Validated-NB100-428-img0011.jpg)
![Immunohistochemistry: FIH-1/HIF-1AN Antibody [NB100-428] - 3D target protein expression in multicellular tumor spheroids3D expression analysis was carried out in the MCF-7, MDA-MB-231 & HBL-100 cell lines using multicellular tumor spheroids. The spheroids were cultured in spinner flasks for 1 week before fixation. Hypoxyprobe was used to examine the formation of hypoxic areas within the spheroids. NHE1, CAIX, MMP14 & FIH-1 expression was also analyzed. Incubation for an hour with PBS instead of primary antibody acted as a control. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.17143), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-428_rabbit-polyclonal-fih-1-hif-1an-antibody-310202415384122.jpg)
![Western Blot: Factor Inhibiting HIF-1 Antibody (162c) [NBP1-30333] - Analysis in A431 cell lysates using NBP1-30333.](http://images.novusbio.com/fullsize/Factor-Inhibiting-HIF-1-Antibody-162c-Western-Blot-NBP1-30333-img0006.jpg "Western Blot: Factor Inhibiting HIF-1 Antibody (162c) [NBP1-30333] - Analysis in A431 cell lysates using NBP1-30333.")
![Immunocytochemistry/Immunofluorescence: Factor Inhibiting HIF-1 Antibody (162c) [NBP1-30333] - FIH (162C) antibody was tested in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Factor-Inhibiting-HIF-1-Antibody-162c-Immunocytochemistry-Immunofluorescence-NBP1-30333-img0005.jpg)
![Immunohistochemistry: Factor Inhibiting HIF-1 Antibody (162c) [NBP1-30333] - Analysis of FIH in human renal cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Factor-Inhibiting-HIF-1-Antibody-162c-Immunohistochemistry-NBP1-30333-img0007.jpg)
![Immunohistochemistry: FIH-1/HIF-1AN Antibody (12F11) [NBP3-26233] - Image of FIH-1/HIF-1AN Antibody (12F11) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26233_rabbit-fih-1-hif-1an-mab-12f11-262202415355734.jpg "Immunohistochemistry: FIH-1/HIF-1AN Antibody (12F11) [NBP3-26233] - Image of FIH-1/HIF-1AN Antibody (12F11) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: FIH-1/HIF-1AN Antibody (12F11) [NBP3-26233] - Image of FIH-1/HIF-1AN Antibody (12F11) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26233_rabbit-fih-1-hif-1an-mab-12f11-272202485636.jpg)
![Western Blot: FIH-1/HIF-1AN Antibody (12F11) [NBP3-26233] - Positive Western Blot detected in: Ntera-2 whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, PC3 whole cell lysate, U87 whole cell lysate. All lanes: FIH-1/HIF-1AN Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 41 kDa Observed band size: 36-53 kDa](http://images.novusbio.com/fullsize/nbp3-26233_rabbit-fih-1-hif-1an-mab-12f11-282202411442647.jpg)
![Immunocytochemistry/Immunofluorescence: FIH-1/HIF-1AN Antibody (12F11) [NBP3-26233] - Staining of HepG2 cell with FIH-1/HIF-1AN Antibody (12F11) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 525-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26233_rabbit-fih-1-hif-1an-mab-12f11-28220241312243.jpg)
![Flow Cytometry: FIH-1/HIF-1AN Antibody (12F11) [NBP3-26233] - Overlay Peak curve showing Hela cells stained with FIH-1/HIF-1AN Antibody (12F11) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26233_rabbit-fih-1-hif-1an-mab-12f11-282202414312744.jpg)
![Immunohistochemistry-Paraffin: FIH-1/HIF-1AN Antibody [NBP2-54749] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/FIH-1-HIF-1AN-Antibody-Immunohistochemistry-Paraffin-NBP2-54749-img0004.jpg "Immunohistochemistry-Paraffin: FIH-1/HIF-1AN Antibody [NBP2-54749] - Staining of human testis shows high expression.")
![Immunohistochemistry-Paraffin: FIH-1/HIF-1AN Antibody [NBP2-54749] - Staining of human testis shows strong nuclear and cytoplasmic positivity in seminiferous ducts.](http://images.novusbio.com/fullsize/FIH-1-HIF-1AN-Antibody-Immunohistochemistry-Paraffin-NBP2-54749-img0002.jpg)
![Immunohistochemistry-Paraffin: FIH-1/HIF-1AN Antibody [NBP2-54749] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/FIH-1-HIF-1AN-Antibody-Immunohistochemistry-Paraffin-NBP2-54749-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FIH-1/HIF-1AN Antibody [NBP2-54749] - Staining in human testis and liver tissues using anti-HIF1AN antibody. Corresponding HIF1AN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FIH-1-HIF-1AN-Antibody-Immunohistochemistry-Paraffin-NBP2-54749-img0005.jpg)
![Western Blot: FIH-1/HIF-1AN Antibody (9R7V4) [NBP3-16256] - Western blot analysis of extracts of U-87MG cells, using FIH-1/HIF-1AN Rabbit mAb (NBP3-16256) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/FIH-1-HIF-1AN-Antibody-9R7V4-Western-Blot-NBP3-16256-img0002.jpg "Western Blot: FIH-1/HIF-1AN Antibody (9R7V4) [NBP3-16256] - Western blot analysis of extracts of U-87MG cells, using FIH-1/HIF-1AN Rabbit mAb (NBP3-16256) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![<b>Genetic Strategies Validation. </b>Knockout Validated: FIH-1/HIF-1AN Antibody (9R7V4) [NBP3-16256] - Western blot analysis of extracts from normal (control) and FIH-1/HIF-1AN knockout (KO) 293T cells, using FIH-1/HIF-1AN antibody (NBP3-16256) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/FIH-1-HIF-1AN-Antibody-9R7V4-Knockout-Validated-NBP3-16256-img0001.jpg)
![Western Blot: FIH-1/HIF-1AN Antibody [NBP2-99356] - Anti-FIH-1/HIF-1AN rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: MCF7 Whole Cell Lysate Lane C: K562 Whole Cell Lysate Lane D: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa](http://images.novusbio.com/fullsize/FIH-1-HIF-1AN-Antibody-Western-Blot-NBP2-99356-img0003.jpg "Western Blot: FIH-1/HIF-1AN Antibody [NBP2-99356] - Anti-FIH-1/HIF-1AN rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: MCF7 Whole Cell Lysate Lane C: K562 Whole Cell Lysate Lane D: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa")
![Immunohistochemistry-Paraffin: FIH-1/HIF-1AN Antibody [NBP2-99356] - Immunochemical staining of human FIH-1/HIF-1AN in human kidney with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/FIH-1-HIF-1AN-Antibody-Immunohistochemistry-Paraffin-NBP2-99356-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: FIH-1/HIF-1AN Antibody [NBP2-58365] - Staining of human cell line MCF7 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/FIH-1-HIF-1AN-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58365-img0001.jpg "Immunocytochemistry/Immunofluorescence: FIH-1/HIF-1AN Antibody [NBP2-58365] - Staining of human cell line MCF7 shows localization to nucleoplasm & cytosol.")
![Western Blot: Factor Inhibiting HIF-1 Overexpression Lysate (Adult Normal) [NBL1-11541] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Factor Inhibiting HIF-1.](http://images.novusbio.com/fullsize/Factor-Inhibiting-HIF-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11541-img0002.jpg "Western Blot: Factor Inhibiting HIF-1 Overexpression Lysate (Adult Normal) [NBL1-11541] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Factor Inhibiting HIF-1.")
![SDS-Page: Recombinant Human FIH-1/HIF-1AN Protein [NBC1-22596] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. Molecular Weight: 40.2 kDa (349 aa), confirmed by MALDI-TOF. Concentration: 1 mg/ml (determined by Bradford assay)](http://images.novusbio.com/fullsize/Recombinant-Human-FIH-1-HIF-1AN-Protein-SDS-Page-NBC1-22596-img0001.jpg "SDS-Page: Recombinant Human FIH-1/HIF-1AN Protein [NBC1-22596] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. Molecular Weight: 40.2 kDa (349 aa), confirmed by MALDI-TOF. Concentration: 1 mg/ml (determined by Bradford assay)")