I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.

Name: CD4 Antibody [Unconjugated]
Brand: Novus Biologicals
SKU: AF-379-NA
Rating: 4 (1 reviews)

Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

CD4 Antibody [Unconjugated] (AF-379-NA): Novus Biologicals

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Publications using AF-379-NA	Applications	Species
Nakamura, S;Ohuchida, K;Hayashi, M;Katayama, N;Tsutsumi, C;Yamada, Y;Hisano, K;Okuda, S;Ohtsubo, Y;Iwamoto, C;Torata, N;Mizuuchi, Y;Shindo, K;Nakata, K;Moriyama, T;Morisaki, T;Oda, Y;Nakamura, M; Tertiary lymphoid structures correlate with enhancement of antitumor immunity in esophageal squamous cell carcinoma British journal of cancer 2023-08-21 [PMID: 37604932] (IHC, Human)	IHC	Human
Lee, AR;Lee, SY;Choi, JW;Um, IG;Na, HS;Lee, JH;Cho, ML; Establishment of a humanized mouse model of keloid diseases following the migration of patient immune cells to the lesion: Patient-derived keloid xenograft (PDKX) model Experimental & molecular medicine 2023-08-01 [PMID: 37524866]
Markus M. Xie, Bingbing Dai, Jason A. Hackney, Tianhe Sun, Juan Zhang, Janet K. Jackman et al. An agonistic anti-signal regulatory protein alpha antibody for chronic inflammatory diseases Cell Reports Medicine 2023-08-15 [PMID: 37490914]
Hrishikesh Pandit, Antonio Valentin, Matthew Angel, Claire Deleage, Cristina Bergamaschi, Jenifer Bear et al. Step-dose IL-7 treatment promotes systemic expansion of T cells and alters immune cell landscape in blood and lymph nodes iScience 2023-02-17 [PMID: 36685042]
R Haddad, O Zlotnik, T Goshen-Lag, M Levi, E Brook, B Brenner, Y Kundel, I Ben-Aharon, H Kashtan Tumor Lymphocyte Infiltration Is Correlated with a Favorable Tumor Regression Grade after Neoadjuvant Treatment for Esophageal Adenocarcinoma Journal of personalized medicine, 2022-04-13;12(4):. 2022-04-13 [PMID: 35455743] (IHC, Human)	IHC	Human
Julien Pothlichet, Annalisa Meola, Florence Bugault, Louise Jeammet, Anne G. Savitt, Berhane Ghebrehiwet et al. Microbial Protein Binding to gC1qR Drives PLA2G1B-Induced CD4 T-Cell Anergy Frontiers in Immunology 2022-03-22 [PMID: 35392090]
K Sangare, S Helmold Ha, M Moore, C Hogge, T Hoang, MA Rahman, DJ Venzon, C LaBranche, D Montefiori, M Robert-Gur, MA Thomas E4orf1 Suppresses E1B-Deleted Adenovirus Vaccine-Induced Immune Responses Vaccines, 2022-02-15;10(2):. 2022-02-15 [PMID: 35214753] (Flow Cyomtetry, Western Blot, Human)	Flow Cyomtetry, Western Blot	Human
AkusjArvi SS, Ambikan AT, Krishnan S Et al. Integrative proteo-transcriptomic and immunophenotyping signatures of HIV-1 elite control phenotype: A cross-talk between glycolysis and HIF signaling iScience 1/10/2022 [PMID: 35005552] Details: Citation using the Alexa Fluor 594 version of this antibody.
Liu W, Danckwardt-Lilliestr�m N, Schrott-Fischer A et al. Distribution of Immune Cells Including Macrophages in the Human Cochlea Frontiers in Neurology 2021-11-22 [PMID: 34880828]
BJ Mehrara, HJ Park, RP Kataru, J Bromberg, M Coriddi, JE Baik, J Shin, C Li, MR Cavalli, EM Encarnacio, M Lee, KJ Van Zee, E Riedel, JH Dayan Pilot Study of Anti-Th2 Immunotherapy for the Treatment of Breast Cancer-Related Upper Extremity Lymphedema Biology, 2021-09-18;10(9):. 2021-09-18 [PMID: 34571811] (IHC, Human)	IHC	Human
Bingying Du, Jiaming Teng, Rongkun Yin, Yuanyuan Tian, Tingwang Jiang, Yanan Du et al. Increased Circulating T Follicular Helper Cells Induced via IL-12/21 in Patients With Acute on Chronic Hepatitis B Liver Failure Frontiers in Immunology 2021-03-31 [PMID: 33868273]
Julien Pothlichet, Thierry Rose, Florence Bugault, Louise Jeammet, Annalisa Meola, Ahmed Haouz et al. PLA2G1B is involved in CD4 anergy and CD4 lymphopenia in HIV-infected patients Journal of Clinical Investigation 2020-04-27 [PMID: 32436864]
Luisa Bell, Alexander Lenhart, Andreas Rosenwald, Camelia M. Monoranu, Friederike Berberich-Siebelt Lymphoid Aggregates in the CNS of Progressive Multiple Sclerosis Patients Lack Regulatory T Cells Frontiers in Immunology 2020-01-15 [PMID: 32010141]
JP Sutherland, M Steain, ME Buckland, M Rodriguez, AL Cunningham, B Slobedman, A Abendroth Persistence of a T Cell Infiltrate in Human Ganglia Years After Herpes Zoster and During Post-herpetic Neuralgia Front Microbiol, 2019-09-11;10(0):2117. 2019-09-11 [PMID: 31572325] (IHC-P, Human)	IHC-P	Human
Liu W, Kampfe Nordstrom C, Danckwardt-Lilliestrom N, Rask-Andersen H, Human Inner Ear Immune Activity: A Super-Resolution Immunohistochemistry Study Front Neurol 7/10/2019 [PMID: 31354608]
Christopher S. Smillie, Moshe Biton, Jose Ordovas-Montanes, Keri M. Sullivan, Grace Burgin, Daniel B. Graham et al. Cellular and inter-cellular rewiring of the human colon during ulcerative colitis Cell 2019-07-01 [PMID: 31348891]
Damond N, Engler S, Zanotelli VRT et al. A Map of Human Type 1 Diabetes Progression by Imaging Mass Cytometry Cell Metab. 1/11/2019 [PMID: 30713109]
Luo Ba, Jintao Du, Feng Liu, Fenglin Yang, Miaomiao Han, Sixi Liu et al. Distinct Inflammatory Profiles in Atopic and Nonatopic Patients With Chronic Rhinosinustis Accompanied by Nasal Polyps in Western China Allergy, Asthma & Immunology Research 2015-04-21 [PMID: 25936910]
Reissfelder C, Stamova S, Gossmann C, Braun M, Bonertz A, Walliczek U, Grimm M, Rahbari N, Koch M, Saadati M, Benner A, Buchler M, Jager D, Halama N, Khazaie K, Weitz J, Beckhove P Tumor-specific cytotoxic T lymphocyte activity determines colorectal cancer patient prognosis. J Clin Invest, 2014-12-22;125(2):739-51. 2014-12-22 [PMID: 25562322] (IHC-Fr, Human)	IHC-Fr	Human
Del Corno M, Donninelli G, Varano B, Da Sacco L, Masotti A, Gessani S HIV-1 gp120 activates the STAT3/interleukin-6 axis in primary human monocyte-derived dendritic cells. J Virol, 2014-07-09;88(19):11045-55. 2014-07-09 [PMID: 25008924] (Neutralization, Human)	Neutralization	Human
Suzuki , Yasuhiro, Gatanaga , Hiroyuki, Tachikawa , Natsuo, Oka , Shinichi Slow turnover of HIV-1 receptors on quiescent CD4+ T cells causes prolonged surface retention of gp120 immune complexes in vivo. PLoS ONE, 2014-02-06;9(2):e86479. 2014-02-06 [PMID: 24516533] (ICC, Human)	ICC	Human
Swaminathan G, Rossi F, Sierra L, Gupta A, Navas-Martin S, Martin-Garcia J A role for microRNA-155 modulation in the anti-HIV-1 effects of Toll-like receptor 3 stimulation in macrophages. PLoS Pathog, 2012-09-20;8(9):e1002937. 2012-09-20 [PMID: 23028330] (ICC, Human)	ICC	Human
Chan Y, Chen K, Duncan S, Lathrop K, Latoche J, Logar A, Pociask D, Wahlberg B, Ray P, Ray A, Pilewski J, Kolls J Patients with cystic fibrosis have inducible IL-17+IL-22+ memory cells in lung draining lymph nodes. J Allergy Clin Immunol, 2012-07-11;131(4):1117-29, 1129. 2012-07-11 [PMID: 22795370] (IHC, Human)	IHC	Human
Ming Zeng, Peter J. Southern, Cavan S. Reilly, Greg J. Beilman, Jeffrey G. Chipman, Timothy W. Schacker et al. Lymphoid Tissue Damage in HIV-1 Infection Depletes Naïve T Cells and Limits T Cell Reconstitution after Antiretroviral Therapy PLoS Pathogens 2012-01-05 [PMID: 22241988]
Fasth AE, Dastmalchi M, Rahbar A, Salomonsson S, Pandya JM, Lindroos E, Nennesmo I, Malmberg KJ, Soderberg-Naucler C, Trollmo C, Lundberg IE, Malmstrom V T cell infiltrates in the muscles of patients with dermatomyositis and polymyositis are dominated by CD28null T cells. J. Immunol., 2009-09-14;183(7):4792-9. 2009-09-14 [PMID: 19752224] (IHC, Human)	IHC	Human
Englund P, Wahlstrom J, Fathi M, Rasmussen E, Grunewald J, Tornling G, Lundberg IE Restricted T cell receptor BV gene usage in the lungs and muscles of patients with idiopathic inflammatory myopathies. Arthritis Rheum., 2007-01-01;56(1):372-83. 2007-01-01 [PMID: 17195241] (IHC, Human)	IHC	Human
Wang B, He J, Liu C, Chang LJ An effective cancer vaccine modality: lentiviral modification of dendritic cells expressing multiple cancer-specific antigens. Vaccine, 2006-02-28;24(17):3477-89. 2006-02-28 [PMID: 16530303] (ICC, Human, Primate)	ICC	Human, Primate
Bjerke SL, Roller RJ Roles for herpes simplex virus type 1 UL34 and US3 proteins in disrupting the nuclear lamina during herpes simplex virus type 1 egress. Virology, 2006-01-19;347(2):261-76. 2006-01-19 [PMID: 16427676] (ICC, Human, Primate - Cholrocebus pygerythrus (Vervet Monkey))	ICC	Human, Primate - Cholrocebus pygerythrus (Vervet Monkey)
Show All 28 Publications. Collapse Publications.

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Details

Immunohistochemistry-Frozen CD4 AF-379-NA

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reviewed by:
Hanane Belhoul-Fakir

IHC-Fr

Porcine

12/03/2020

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Summary

Application	Immunohistochemistry-Frozen
Sample Tested	Porcine spleen
Species	Porcine
Lot	YS0719061

Comments

Pig spleen frozen OCT tissues were sectioned at 7µm and fixed in ice cold acetone for 7min. Once dry, blocking buffer 4% FCS/TBS was added to all tissues for 30min to minimize non-specific binding, followed by 10 min incubation with Bloxall, endogenous peroxidase blocking solution at room temperature. Tissues were washed in Tris buffered saline (TBS) for 5 min and incubated for 20min with normal horse serum (Vector laboratories # S-2012-50). Human Polyclonal goat anti-CD4 (Novus biologicals # AF379-NA) was diluted at 1:50 in 0.5% FCS/TBS and added to the arterial tissues for 1 hour incubation at room temperature. Following 5 min wash in TBS, tissues were incubated for 30min with ImmPRESS®HRP horse anti-goat reagent (Vector laboratories # MP-7405). To detect CD4 antigen, tissues were washed in TBS and incubated with VIP HRP substrate (Vector®VIP Substrate kit, Peroxidase (HRP) # SK-4600) for 1 min 30sec then placed immediately in MilliQ water to stop the reaction. Nuclei were counter-stained with methyl green (Sigma-Aldrich # M8884-25G). Control tissues were stained similarly in the absence of the primary antibody. Tissue sections were immediately dehydrated and mounted with ENTELLAN mounting medium (Proscitech, Cat#IA013). Images were captured on Nikon eclipse ME600 microscope and DS-Fi2 camera.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	WB, Simple Western, IHC, ICC/IF
Clonality	Polyclonal
Host	Goat
Conjugate	Unconjugated
Concentration	LYOPH

Catalog# & Conjugate	Size	Price
Alexa Fluor 488 FAB8165G

Alexa Fluor 700 FAB8165N-100

Biotin BAF379

Catalog# & Formulation	Size	Price
Carrier Free FAB8165G

Carrier Free AF-379-SP

Carrier Free FAB8165N-100

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 488	FAB8165G
Alexa Fluor 700	FAB8165N-100
Biotin	BAF379

CD4 Antibody [Unconjugated]

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Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD4 Lys26-Trp390 Accession # P01730
Specificity	Detects human CD4 in direct ELISAs and Western blots.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Goat
Gene	CD4
Purity Statement	Antigen Affinity-purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS.

	Hanane Belhoul-Fakir 12/03/2020
Application:	IHC-Fr
Species:	Porcine

Gene Symbol	CD4
Uniprot	P01730()