Goat IgG Isotype Control [Unconjugated]

Images

 
SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/RatEphrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog #AF496,filled histogram) or Normal Goat IgG Isotype Control ...read more
Decreased macrophage recruitment correlates with decreased angiogenesis.A) Quantification of BrdU incorporation demonstrates that decreased macrophage infiltration does not significantly correlate with a change in ...read more
iFGFR1 activation in vivo promotes recruitment of CX3CR1-positive macrophages.MMTV-iFGFR1 transgenic mice were treated with B/B in order to analyze the population of macrophages that are recruited to the mammary ...read more
iFGFR1 activation in vivo promotes recruitment of CX3CR1-positive macrophages.MMTV-iFGFR1 transgenic mice were treated with B/B in order to analyze the population of macrophages that are recruited to the mammary ...read more

Product Details

Summary
Reactivity GtSpecies Glossary
Applications Ctrl
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Goat IgG Isotype Control [Unconjugated] Summary

Immunogen
NA
Specificity
Serum was obtained from naive (non-immunized) goats and purified for use as normal goat IgG.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGHG1
Purity Statement
Protein A or G purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

Dilutions
  • Control
Reviewed Applications
Read 2 Reviews rated 5
using
AB-108-C in the following application:

Publications
Read Publications using
AB-108-C in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Dissolve the lyophilized goat IgG in sterile PBS, pH 7.4. If 1 mL of PBS is used, the antibody concentration will be 1 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Goat IgG Isotype Control [Unconjugated]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG Isotype Control (AB-108-C)(616)

We have publications tested in 18 confirmed species: Human, Mouse, Rat, Bacteria - Streptococcus pyogenes, Bovine, Canine, Chicken, Ferret, Guinea Pig, Hamster, Porcine, Primate, Primate - C. aethiops, Primate - Macaca mulatta (Rhesus Macaque), Rabbit, Transgenic Mouse, Transgenic Rat, Xenograft.

We have publications tested in 35 applications: Binding Assay, Binding Control, Bioassay, Blocking, Cell Culture, ChIP, ChIP (chromatin immunoprecipit, ChIP Control, Co-Immunoprecipitation, Control, ELISA Control, Flow Cytometry, Flow Cytometry Control, Functional Assay, Functional Assay - Control, Functional Assay Control, ICC, ICC Control, IHC, IHC Control, IHC-F, IHC-Fr, IHC-P, Immunoprecipitation, Immunoprecipitation Control, In Vivo, In Vivo Control, Isotype Control, Neutralization, Neutralization Control, RNA Immunoprecipitation Contro, RNA immunoprecipitation, Tissue Culture, Western Blot, Western Blot Control.


Filter By Application
Binding Assay
(1)
Binding Control
(1)
Bioassay
(12)
Blocking
(1)
Cell Culture
(6)
ChIP
(6)
ChIP (chromatin immunoprecipit
(1)
ChIP Control
(1)
Co-Immunoprecipitation
(2)
Control
(118)
ELISA Control
(1)
Flow Cytometry
(17)
Flow Cytometry Control
(4)
Functional Assay
(7)
Functional Assay - Control
(1)
Functional Assay Control
(1)
ICC
(8)
ICC Control
(2)
IHC
(17)
IHC Control
(8)
IHC-F
(1)
IHC-Fr
(1)
IHC-P
(5)
Immunoprecipitation
(9)
Immunoprecipitation Control
(3)
In Vivo
(16)
In Vivo Control
(4)
Isotype Control
(34)
Neutralization
(53)
Neutralization Control
(44)
RNA Immunoprecipitation Contro
(1)
RNA immunoprecipitation
(1)
Tissue Culture
(1)
Western Blot
(5)
Western Blot Control
(1)
All Applications
Filter By Species
Human
(170)
Mouse
(180)
Rat
(25)
Bacteria - Streptococcus pyogenes
(1)
Bovine
(1)
Canine
(1)
Chicken
(2)
Ferret
(1)
Guinea Pig
(1)
Hamster
(2)
Porcine
(4)
Primate
(1)
Primate - C. aethiops
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(2)
Rabbit
(1)
Transgenic Mouse
(4)
Transgenic Rat
(1)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 616. Show All 616 Publications.
Publications using AB-108-C Applications Species
Justynski, O;Bridges, K;Krause, W;Forni, MF;Phan, QM;Sandoval-Schaefer, T;Carter, K;King, DE;Hsia, HC;Gazes, MI;Vyce, SD;Driskell, RR;Miller-Jensen, K;Horsley, V; Apoptosis recognition receptors regulate skin tissue repair in mice eLife 2023-12-21 [PMID: 38127424] (In Vivo, Mouse) In Vivo Mouse
Rogala, S;Ali, T;Melissari, MT;Währisch, S;Schuster, P;Sarre, A;Emídio, RC;Boettger, T;Rogg, EM;Kaur, J;Krishnan, J;Dumbovi?, G;Dimmeler, S;Ounzain, S;Pedrazzini, T;Herrmann, BG;Grote, P; The lncRNA Sweetheart regulates compensatory cardiac hypertrophy after myocardial injury in murine males Nature communications 2023-11-02 [PMID: 37919291] (RNA Immunoprecipitation Contro, Mouse) RNA Immunoprecipitation Contro Mouse
Antwi, MB;Dumitriu, G;Simón-Santamaria, J;Romano, JS;Li, R;Smedsrød, B;Vik, A;Eskild, W;Sørensen, KK; Liver sinusoidal endothelial cells show reduced scavenger function and downregulation of Fc gamma receptor IIb, yet maintain a preserved fenestration in the Glmpgt/gt mouse model of slowly progressing liver fibrosis PloS one 2023-11-01 [PMID: 37910485] (IHC, Mouse, Transgenic Mouse) IHC Mouse, Transgenic Mouse
Chandrasekar, B;Mummidi, S;DeMarco, VG;Higashi, Y; Empagliflozin Reverses Oxidized LDL-Induced RECK Suppression, Cardiotrophin-1 Expression, MMP Activation, and Human Aortic Smooth Muscle Cell Proliferation and Migration Mediators of inflammation 2023-10-04 [PMID: 37830075] (Western Blot Control, Human) Western Blot Control Human
Di Carlo, SE;Raffenne, J;Varet, H;Ode, A;Granados, DC;Stein, M;Legendre, R;Tuckermann, J;Bousquet, C;Peduto, L; Depletion of slow-cycling PDGFR?+ADAM12+ mesenchymal cells promotes antitumor immunity by restricting macrophage efferocytosis Nature immunology 2023-10-05 [PMID: 37798557] (In Vivo Control, Mouse) In Vivo Control Mouse
De Monte, L;Clemente, F;Ruggiero, E;Pini, R;Ceraolo, MG;Schiavo Lena, M;Balestrieri, C;Lazarevic, D;Belfiori, G;Crippa, S;Balzano, G;Falconi, M;Doglioni, C;Bonini, C;Reni, M;Protti, MP; Pro-tumor Tfh2 cells induce detrimental IgG4 production and PGE2-dependent IgE inhibition in pancreatic cancer EBioMedicine 2023-09-28 [PMID: 37776595] (Neutralization Control, Human) Neutralization Control Human
Molfetta, R;Lecce, M;Milito, ND;Putro, E;Pietropaolo, G;Marangio, C;Scarno, G;Moretti, M;De Smaele, E;Santini, T;Bernardini, G;Sciumè, G;Santoni, A;Paolini, R; SCF and IL-33 regulate mouse mast cell phenotypic and functional plasticity supporting a pro-inflammatory microenvironment Cell death & disease 2023-09-20 [PMID: 37730723] (Neutralization Control, Mouse) Neutralization Control Mouse
Militi, S;Nibhani, R;Jalali, M;Pauklin, S; RBL2-E2F-GCN5 guide cell fate decisions during tissue specification by regulating cell-cycle-dependent fluctuations of non-cell-autonomous signaling Cell reports 2023-09-18 [PMID: 37725511] (ICC Control, Human) ICC Control Human
Rogers, S;Zhang, C;Anagnostidis, V;Liddle, C;Fishel, ML;Gielen, F;Scholpp, S; Cancer-associated fibroblasts influence Wnt/PCP signaling in gastric cancer cells by cytoneme-based dissemination of ROR2 Proceedings of the National Academy of Sciences of the United States of America 2023-09-26 [PMID: 37722040] (ICC, Human) ICC Human
María Belén Giorello, Leandro Marcelo Martinez, Francisco Raúl Borzone, María Del Rosario Padin, María Florencia Mora, Ina Sevic et al. CD105 expression in cancer-associated fibroblasts: a biomarker for bone metastasis in early invasive ductal breast cancer patients Front Cell Dev Biol 2023-08-31 [PMID: 37719885]
Show All 616 Publications.

Reviews for IgG Isotype Control (AB-108-C) (2) 52

Average Rating: 5
(Based on 2 reviews)

Reviews using AB-108-C:
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
05/07/2019
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Summary

Lotes4118071
 IgG AB-108-C
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5
reviewed by:
Verified Customer
04/07/2017
View

Summary

LotES4116081

Comments

CommentsUsed ad an in vivo control. [In vivo] EAE was induced in C57BL/6 mice by immunization with an emulsion of MOG35-55 in complete Freund's adjuvant (CFA), followed by administration of pertussis toxin (PTX) in PBS, first on the day of immunization and then again 2 days later. Mice were then injected with a total of 40ug Normal Goat IgG (R&D AB108C) vs. PBS (100ul 1x PBS). A 100ul volume of 10ug Normal Goat IgG was injected intravenously every other day starting at day 13 post MOG for a total of 4 injections. Clinical scores were measured daily using the following scoring scale: 0) Normal mouse; no overt signs of disease; 1) Limp tail or hind limb weakness; 2)Limp tail and hind limb weakness; 3) Partial hind limb paralysis; 4)Complete hind limb paralysis; 5) Moribund state; death by EAE.

FAQs for IgG Isotype Control (AB-108-C) (0)

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05/07/2019
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Bioinformatics

Gene Symbol IGHG1