Name: CCR5 Antibody (45531) [Unconjugated]
Brand: Novus Biologicals
SKU: MAB182
Rating: 4 (1 reviews)

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reviewed by:
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Flow

Human

08/28/2018

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Summary

Application	Flow Cytometry
Sample Tested	Peripheral blood mononuclear cells (PBMCs)
Species	Human
Lot	AWK0717121

Comments

Freshly isolated human PBMCs were stained with mouse anti-human CCR5 monoclonal antibody (MAB182) or mIgG2b isotype control at 2.5 µg/ml for 30 minutes on ice followed by goat anti-mouse IgG PE secondary staining. Additional CD3 APC staining.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	Flow, B/N, CyTOF-ready, ICC/IF
Clone	45531
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated
Concentration	LYOPH

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 FAB182U-100UG

Alexa Fluor 405 FAB182V-100UG

Alexa Fluor 488 FAB182G-100UG

Alexa Fluor 532 FAB182X

Alexa Fluor 594 FAB182T-100UG

Alexa Fluor 647 FAB182R-100UG

Alexa Fluor 700 FAB182N-100UG

Alexa Fluor 750 FAB182S-100UG

Allophycocyanin FAB182A

Allophycocyanin/Cy7 FAB182APCCY7

CoraFluor 1 FAB182CL1

DyLight 350 FAB182D

DyLight 405 FAB182E

DyLight 488 FAB182K

DyLight 550 FAB182L

DyLight 594 FAB182M

DyLight 650 FAB182W

DyLight 680 FAB182Y

DyLight 755 FAB182Z

Fluorescein FAB182F-025

Fluorescein FAB182F-100

HRP FAB182H

Janelia Fluor 525 FAB182JF525

Janelia Fluor 549 FAB182I

Janelia Fluor 585 FAB182JF585

Janelia Fluor 635 FAB182JF635

Janelia Fluor 646 FAB182J

Janelia Fluor 669 FAB182JF669

PE/Atto594 FAB182PEATT594

PE/Cy5.5 FAB182PECY55

PE/Cy7 FAB182PECY7

PerCP FAB182C

Phycoerythrin FAB182P-100

Phycoerythrin FAB182P-025

mFluor Violet 450 SE FAB182MFV450

mFluor Violet 500 SE FAB182MFV500

mFluor Violet 610 SE FAB182MFV610

Catalog# & Formulation	Size	Price
Carrier Free FAB182F-025

Carrier Free FAB182F-100

Carrier Free FAB182P-025

Carrier Free FAB182P-100

Carrier Free MAB182-SP

Carrier Free FAB182V-100UG

Carrier Free FAB182U-100UG

Carrier Free FAB182T-100UG

Carrier Free FAB182S-100UG

Carrier Free FAB182R-100UG

Carrier Free FAB182N-100UG

Carrier Free FAB182G-100UG

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB182U-100UG
Alexa Fluor 405	FAB182V-100UG
Alexa Fluor 488	FAB182G-100UG
Alexa Fluor 532	FAB182X
Alexa Fluor 594	FAB182T-100UG
Alexa Fluor 647	FAB182R-100UG
Alexa Fluor 700	FAB182N-100UG
Alexa Fluor 750	FAB182S-100UG
Allophycocyanin	FAB182A
Allophycocyanin/Cy7	FAB182APCCY7
CoraFluor 1	FAB182CL1
DyLight 350	FAB182D
DyLight 405	FAB182E
DyLight 488	FAB182K
DyLight 550	FAB182L
DyLight 594	FAB182M
DyLight 650	FAB182W
DyLight 680	FAB182Y
DyLight 755	FAB182Z
Fluorescein	FAB182F-025
Fluorescein	FAB182F-100
HRP	FAB182H
Janelia Fluor 525	FAB182JF525
Janelia Fluor 549	FAB182I
Janelia Fluor 585	FAB182JF585
Janelia Fluor 635	FAB182JF635
Janelia Fluor 646	FAB182J
Janelia Fluor 669	FAB182JF669
PE/Atto594	FAB182PEATT594
PE/Cy5.5	FAB182PECY55
PE/Cy7	FAB182PECY7
PerCP	FAB182C
Phycoerythrin	FAB182P-100
Phycoerythrin	FAB182P-025
mFluor Violet 450 SE	FAB182MFV450
mFluor Violet 500 SE	FAB182MFV500
mFluor Violet 610 SE	FAB182MFV610

CCR5 Antibody (45531) [Unconjugated]

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CCR5 Antibody (45531) [Unconjugated] Summary

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Publications for CCR5 Antibody (MAB182)(32)

Review for CCR5 Antibody (MAB182) (1) 41

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Immunogen	NS0 mouse myeloma cell line transfected with human CCR5 Met1-Leu352 Accession # P51681
Specificity	Detects human CCR5.
Source	N/A
Isotype	IgG2b
Clonality	Monoclonal
Host	Mouse
Gene	CCR5
Purity Statement	Protein A or G purified from ascites
Endotoxin Note	<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.5 mg/mL in sterile PBS.

Publications using MAB182	Applications	Species
Lee, JW;Lee, IH;Watanabe, H;Liu, Y;Sawada, K;Maekawa, M;Uehara, S;Kobayashi, Y;Imai, Y;Kong, SW;Iimura, T; Centrosome clustering control in osteoclasts through CCR5-mediated signaling Scientific reports 2023-11-27 [PMID: 38012303] (Neutralization, Human)	Neutralization	Human
M Weichseldo, Y Tagaya, M Reitz, AL DeVico, OS Latinovic Identifying CCR5 coreceptor populations permissive for HIV-1 entry and productive infection: implications for in vivo studies Journal of Translational Medicine, 2022-01-24;20(1):39. 2022-01-24 [PMID: 35073923] (ICC, Human)	ICC	Human
MC Mladinich, JN Conde, WR Schutt, SY Sohn, ER Mackow Blockade of Autocrine CCL5 Responses Inhibits Zika Virus Persistence and Spread in Human Brain Microvascular Endothelial Cells MBio, 2021-08-17;12(4):e0196221. 2021-08-17 [PMID: 34399621] (Neutralization, Human)	Neutralization	Human
C Jacquemard, F Koensgen, P Colin, B Lagane, E Kellenberg Modeling of CCR5 Recognition by HIV-1 gp120: How the Viral Protein Exploits the Conformational Plasticity of the Coreceptor Viruses, 2021-07-18;13(7):. 2021-07-18 [PMID: 34372601] (Flow Cytometry, Human)	Flow Cytometry	Human
YF Sun, L Wu, SP Liu, MM Jiang, B Hu, KQ Zhou, W Guo, Y Xu, Y Zhong, XR Zhou, ZF Zhang, G Liu, S Liu, YH Shi, Y Ji, M Du, NN Li, GB Li, ZK Zhao, XY Huang, LQ Xu, QC Yu, DH Peng, SJ Qiu, HC Sun, M Dean, XD Wang, WY Chung, AR Dennison, J Zhou, Y Hou, J Fan, XR Yang Dissecting spatial heterogeneity and the immune-evasion mechanism of CTCs by single-cell RNA-seq in hepatocellular carcinoma Nature Communications, 2021-07-02;12(1):4091. 2021-07-02 [PMID: 34215748] (IHC, Human)	IHC	Human
Denarda Dangaj, Marine Bruand, Alizée J. Grimm, Catherine Ronet, David Barras, Priyanka A. Duttagupta et al. Cooperation between Constitutive and Inducible Chemokines Enables T Cell Engraftment and Immune Attack in Solid Tumors Cancer Cell 6/10/2019 [PMID: 31185212]
G Nishikawa, K Kawada, J Nakagawa, K Toda, R Ogawa, S Inamoto, R Mizuno, Y Itatani, Y Sakai Bone marrow-derived mesenchymal stem cells promote colorectal cancer progression via CCR5 Cell Death Dis, 2019-03-19;10(4):264. 2019-03-19 [PMID: 30890699] (Flow Cytometry, Human)	Flow Cytometry	Human
Bray Denard, Sungwon Han, JungYeon Kim, Elliott M Ross, Jin Ye Regulating G protein-coupled receptors by topological inversion eLife 3/5/2019 [PMID: 30835201]
P Colin, Z Zhou, I Staropoli, J Garcia-Per, R Gasser, M Armani-Tou, Y Benureau, N Gonzalez, J Jin, BJ Connell, S Raymond, P Delobel, J Izopet, H Lortat-Jac, J Alcami, F Arenzana-S, A Brelot, B Lagane CCR5 structural plasticity shapes HIV-1 phenotypic properties PLoS Pathog., 2018-12-06;14(12):e1007432. 2018-12-06 [PMID: 30521629] (Competitive Binding Assay, Human)	Competitive Binding Assay	Human
JW Lee, A Hoshino, K Inoue, T Saitou, S Uehara, Y Kobayashi, S Ueha, K Matsushima, A Yamaguchi, Y Imai, T Iimura The HIV co-receptor CCR5 regulates osteoclast function Nat Commun, 2017-12-20;8(1):2226. 2017-12-20 [PMID: 29263385] (Neutralization, Mouse)	Neutralization	Mouse
WL Wu, CR Grotefend, MT Tsai, YL Wang, V Radic, H Eoh, IC Huang ?20 IFITM2 differentially restricts X4 and R5 HIV-1 Proc. Natl. Acad. Sci. U.S.A., 2017-06-19;114(27):7112-7117. 2017-06-19 [PMID: 28630320] (Flow Cytometry, ICC, Human)	Flow Cytometry, ICC	Human
James M. Fox, Richard Kasprowicz, Oliver Hartley, Nathalie Signoret CCR5 susceptibility to ligand-mediated down-modulation differs between human T lymphocytes and myeloid cells Journal of Leukocyte Biology 2015-07-01 [PMID: 25957306]
Ward , S T, Li , K K, Hepburn , E, Weston , C J, Curbishley , S M, Reynolds , G M, Hejmadi , R K, Bicknell , R, Eksteen , B, Ismail , T, Rot , A, Adams , D H The effects of CCR5 inhibition on regulatory T-cell recruitment to colorectal cancer. Br J Cancer, 2014-11-18;112(2):319-28. 2014-11-18 [PMID: 25405854] (IHC-P, Human)	IHC-P	Human
Hartmann N, Giese N, Giese T, Poschke I, Offringa R, Werner J, Ryschich E Prevailing role of contact guidance in intrastromal T-cell trapping in human pancreatic cancer. Clin Cancer Res, 2014-04-24;20(13):3422-33. 2014-04-24 [PMID: 24763614] (Flow Cytometry, Human)	Flow Cytometry	Human
Nakano Y, Monde K, Terasawa H, Yuan Y, Yusa K, Harada S, Maeda Y Preferential recognition of monomeric CCR5 expressed in cultured cells by the HIV-1 envelope glycoprotein gp120 for the entry of R5 HIV-1. Virology, 2014-01-31;452(0):117-24. 2014-01-31 [PMID: 24606688] (Flow Cytometry, Human)	Flow Cytometry	Human
Flegler A, Cianci G, Hope T CCR5 conformations are dynamic and modulated by localization, trafficking and G protein association. PLoS ONE, 2014-02-28;9(2):e89056. 2014-02-28 [PMID: 24586501] (IHC-Fr, Human)	IHC-Fr	Human
Alonzo , Francis, Kozhaya , Lina, Rawlings , Stephen, Reyes-Robles , Tamara, DuMont , Ashley L, Myszka , David G, Landau , Nathanie, Unutmaz , Derya, Torres , Victor J CCR5 is a receptor for Staphylococcus aureus leukotoxin ED. Nature, 2012-12-12;493(7430):51-5. 2012-12-12 [PMID: 23235831] (Bioassay, Human)	Bioassay	Human
Duluc , D, Gannevat , J, Anguiano , E, Zurawski , S, Carley , M, Boreham , M, Stecher , J, Dullaers , M, Banchereau , J, Oh , S Functional diversity of human vaginal APC subsets in directing T-cell responses. Mucosal Immunol, 2012-11-07;6(3):626-38. 2012-11-07 [PMID: 23131784] (Flow Cytometry, Human)	Flow Cytometry	Human
Jung DW, Che ZM, Kim J, Kim K, Kim KY, Williams D, Kim J Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7. Int. J. Cancer, 2010-07-15;127(2):332-44. 2010-07-15 [PMID: 19937793] (Neutralization, Human)	Neutralization	Human
Franciszkiewicz K, Le Floc'h A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention. Cancer Res., 2009-07-28;69(15):6249-55. 2009-07-28 [PMID: 19638592] (Flow Cytometry, Human)	Flow Cytometry	Human
Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases. J. Immunol., 2008-12-01;181(11):8053-67. 2008-12-01 [PMID: 19017998] (Flow Cytometry, ICC, IHC-P, Neutralization, Human)	Flow Cytometry, ICC, IHC-P, Neutralization	Human
Aldinucci D, Lorenzon D, Cattaruzza L, Pinto A, Gloghini A, Carbone A, Colombatti A Expression of CCR5 receptors on Reed-Sternberg cells and Hodgkin lymphoma cell lines: involvement of CCL5/Rantes in tumor cell growth and microenvironmental interactions. Int. J. Cancer, 2008-02-15;122(4):769-76. 2008-02-15 [PMID: 17935139] (Flow Cytometry, ICC, IHC-P, Human)	Flow Cytometry, ICC, IHC-P	Human
Bolovan-Fritts CA, Spector SA Endothelial damage from cytomegalovirus-specific host immune response can be prevented by targeted disruption of fractalkine-CX3CR1 interaction. Blood, 2007-09-25;111(1):175-82. 2007-09-25 [PMID: 17895402] (Neutralization, Human)	Neutralization	Human
Kang SG, Lim HW, Andrisani OM, Broxmeyer HE, Kim CH Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells. J. Immunol., 2007-09-15;179(6):3724-33. 2007-09-15 [PMID: 17785809] (Flow Cytometry, Human)	Flow Cytometry	Human
Abonyo BO, Lebby KD, Tonry JH, Ahmad M, Heiman AS Modulation of eotaxin-3 (CCL26) in alveolar type II epithelial cells. Cytokine, 2007-03-09;36(5):237-44. 2007-03-09 [PMID: 17350279] (Neutralization, Human)	Neutralization	Human
Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. Clin. Immunol., 2006-06-16;120(2):220-31. 2006-06-16 [PMID: 16782408] (Neutralization, Human)	Neutralization	Human
Lepej SZ, Rode OD, Jeren T, Vince A, Remenar A, Barsic B Increased expression of CXCR3 and CCR5 on memory CD4+ T-cells migrating into the cerebrospinal fluid of patients with neuroborreliosis: the role of CXCL10 and CXCL11. J. Neuroimmunol., 2005-04-19;163(1):128-34. 2005-04-19 [PMID: 15885315] (Flow Cytometry, Human)	Flow Cytometry	Human
Lim HW, Hillsamer P, Kim CH Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses. J. Clin. Invest., 2004-12-01;114(11):1640-9. 2004-12-01 [PMID: 15578096] (ICC, Human)	ICC	Human
Ottonello L, Montecucco F, Bertolotto M, Arduino N, Mancini M, Corcione A, Pistoia V, Dallegri F CCL3 (MIP-1alpha) induces in vitro migration of GM-CSF-primed human neutrophils via CCR5-dependent activation of ERK 1/2. Cell. Signal., 2005-03-01;17(3):355-63. 2005-03-01 [PMID: 15567066] (Flow Cytometry, Human)	Flow Cytometry	Human
Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS. J. Leukoc. Biol., 2003-05-01;73(5):584-90. 2003-05-01 [PMID: 12714572] (Neutralization, Human)	Neutralization	Human
Howard OM, Dong HF, Yang D, Raben N, Nagaraju K, Rosen A, Casciola-Rosen L, Hartlein M, Kron M, Yiadom K, Dwivedi S, Plotz PH, Oppenheim JJ Histidyl-tRNA synthetase and asparaginyl-tRNA synthetase, autoantigens in myositis, activate chemokine receptors on T lymphocytes and immature dendritic cells. J. Exp. Med., 2002-09-16;196(6):781-91. 2002-09-16 [PMID: 12235211] (IHC-Fr, Neutralization, Human)	IHC-Fr, Neutralization	Human
Lee B, Sharron M, Blanpain C, Doranz BJ, Vakili J, Setoh P, Berg E, Liu G, Guy HR, Durell SR, Parmentier M, Chang CN, Price K, Tsang M, Doms RW Epitope mapping of CCR5 reveals multiple conformational states and distinct but overlapping structures involved in chemokine and coreceptor function. J. Biol. Chem., 1999-04-02;274(14):9617-26. 1999-04-02 [PMID: 10092648] (Flow Cytometry, Human)	Flow Cytometry	Human
Show All 32 Publications. Collapse Publications.

Gene Symbol	CCR5
Uniprot	P51681()