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ISG20 Antibody (1B2-3C9)

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 (1 Review)
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Western Blot: ISG20 Antibody (1B2-3C9) [H00003669-M01] - BT-474, HCC1954 and SUM190 whole cell lysates were loaded with 40 ug/lane. 10% SDS-PAGE. ISG20 Antibody (H00003669-M01) was used for primary antibody: 1:1000, 4C, overnight. WB image submitted by a verified customer review.
Western Blot: ISG20 Antibody (1B2-3C9) [H00003669-M01] - BT-474, HCC1954 and SUM190 whole cell lysates were loaded with 40 ug/lane. 10% SDS-PAGE. ISG20 Antibody (H00003669-M01) was used for primary antibody: 1:1000, 4C, ...read more
Immunocytochemistry/ Immunofluorescence: ISG20 Antibody (1B2-3C9) [H00003669-M01] - Analysis of monoclonal antibody to ISG20 on HeLa cell . Antibody concentration 10 ug/ml.
Western Blot: ISG20 Antibody (1B2-3C9) [H00003669-M01] - Analysis of ISG20 expression in transfected 293T cell line by ISG20 monoclonal antibody (M01), clone 1B2-3C9.Lane 1: ISG20 transfected lysate(20.4 KDa).Lane 2: ...read more
Sandwich ELISA: ISG20 Antibody (1B2-3C9) [H00003669-M01] - Detection limit for recombinant GST tagged ISG20 is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
1B2-3C9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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ISG20 Antibody (1B2-3C9) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
ISG20 (AAH07922.1, 1 a.a. ~ 181 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAGSREVVAMDCEMVGLGPHRESGLARCSLVNVHGAVLYDKFIRPEGEITDYRTRVSGVTPQHMVGATPFAVARLEILQLLKGKLVVGHDLKHDFQALKEDMSGYTIYDTSTDRLLWREAKLDHCRRVSLRVLSERLLHKSIQNSLLGHSSVEDARATMELYQISQRIRARRGLPRLAVSD
Specificity
ISG20 - interferon stimulated gene 20kDa
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ISG20
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactivity against recombinant protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. It has been use for IF.
Reviewed Applications
Read 1 Review rated 5
using
H00003669-M01 in the following applications:
Publications
Read Publications using H00003669-M01.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ISG20 Antibody (1B2-3C9)

  • CD25
  • Estrogen-regulated transcript 45 protein
  • HEM45EC 3.1.13.1
  • interferon stimulated exonuclease gene 20kDa
  • interferon stimulated gene (20kD)
  • interferon-stimulated gene 20 kDa protein
  • Promyelocytic leukemia nuclear body-associated protein ISG20

Background

Exonuclease with specificity for single-stranded RNA and, to a lesser extent for DNA. Degrades RNA at a rate that is approximately 35-fold higher than its rate for single-stranded DNA. Involved in the antiviral function of IFN against RNA viruses

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ISG20 Antibody (H00003669-M01)(2)


Review for ISG20 Antibody (H00003669-M01) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using H00003669-M01:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Yongkang Yang
 WB Human 09/12/2021
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Summary

ApplicationWestern Blot
Sample Testedbreast cancer cell line, Sample Amount: 40
SpeciesHuman
LotK9211-S3

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Recent Reviews

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Western Blot ISG20 H00003669-M01
Enlarge
 Yongkang Yang
09/12/2021
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol ISG20
Entrez
OMIM
Uniprot