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CD4 Antibody [Alexa Fluor® 488]

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Human peripheral blood lymphocytes were stained with Goat Anti-Human CD4 Alexa Fluor® 488-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB8165G, filled histogram) or isotype control antibody ...read more
CD4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human CD4 Alexa Fluor® 488-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB8165G, green) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Alexa Fluor 488

Order Details

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View Available Formulations

CD4 Antibody [Alexa Fluor® 488] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD4
Lys26-Trp390
Accession # P01730
Specificity
Detects human CD4 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse CD4, recombinant feline CD4, recombinant cotton rat CD4, and recombinant canine CD4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CD4
Purity
Antigen Affinity-purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 5 uL/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
Publications
Read Publications using
FAB8165G in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide
Purity
Antigen Affinity-purified

Notes


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD4 Antibody [Alexa Fluor® 488]

  • CD_antigen: CD4
  • CD4 antigen (p55)
  • CD4 antigen
  • CD4 molecule
  • CD4 receptor
  • CD4
  • CD4mut
  • T-cell surface antigen T4/Leu-3
  • T-cell surface glycoprotein CD4

Background

CD4 is an approximately 55 kDa type I membrane glycoprotein that is expressed predominantly on most thymocytes and a subset of mature T lymphocytes. In humans, CD4 is also expressed to a lesser extent on monocytes and macrophage related cells. Human CD4 cDNA encodes a 458 amino acid (aa) residue precursor protein with a 25 aa residue signal peptide, a 371 aa residue extracellular region containing four immunoglobulin homology domains, a 24 aa residue transmembrane domain and a 38 aa residue cytoplasmic domain. CD4 is a coreceptor required for T cell recognition of antigens that are presented by class II major histocompatibility complexes. CD4 has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

  1. Capon, D.I. et al. (1991) Annu. Rev. Immunol. 9:649.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD4 Antibody (FAB8165G)(13)

We have publications tested in 4 confirmed species: Human, Primate, Primate - Macaca mulatta (Rhesus Macaque), Rhesus Macaque.

We have publications tested in 3 applications: Confocal Microscopy, ICC, IHC.


Filter By Application
Confocal Microscopy
(1)
ICC
(1)
IHC
(2)
All Applications
Filter By Species
Human
(1)
Primate
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
Rhesus Macaque
(1)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using FAB8165G Applications Species
Hua-Jun Wu, Daniel Temko, Zoltan Maliga, Andre L. Moreira, Emi Sei, Darlan Conterno Minussi et al. Spatial intra-tumor heterogeneity is associated with survival of lung adenocarcinoma patients Cell Genomics 2022-08-10 [PMID: 36419822]
Sebastian Jarosch, Jan Köhlen, Sabrina Wagner, Elvira D’Ippolito, Dirk H. Busch ChipCytometry for multiplexed detection of protein and mRNA markers on human FFPE tissue samples STAR Protocols 2022-06-01 [PMID: 35586313]
Jarosch S, KOhlen J, Sarker R Et al. Multiplexed imaging and automated signal quantification in formalin-fixed paraffin-embedded tissues by ChipCytometry Cell Rep Methods 4/27/2022 [PMID: 35475000]
A Pegu, L Xu, ME DeMouth, G Fabozzi, K March, CG Almasri, MD Cully, K Wang, ES Yang, J Dias, CM Fennessey, J Hataye, RR Wei, E Rao, JP Casazza, W Promsote, M Asokan, K McKee, SD Schmidt, X Chen, C Liu, W Shi, H Geng, KE Foulds, SF Kao, A Noe, H Li, GM Shaw, T Zhou, C Petrovas, JP Todd, BF Keele, JD Lifson, NA Doria-Rose, RA Koup, ZY Yang, GJ Nabel, JR Mascola Potent anti-viral activity of a trispecific HIV neutralizing antibody in SHIV-infected monkeys Cell Reports, 2022-01-04;38(1):110199. 2022-01-04 [PMID: 34986348] (IHC, Rhesus Macaque) IHC Rhesus Macaque
Megan L. Burger, Amanda M. Cruz, Grace E. Crossland, Giorgio Gaglia, Cecily C. Ritch, Sarah E. Blatt et al. Antigen dominance hierarchies shape TCF1+ progenitor CD8 T cell phenotypes in tumors Cell 2021-09-13 [PMID: 34534464]
Koikawa K, Kibe S, Suizu F Et al. Targeting Pin1 renders pancreatic cancer eradicable by synergizing with immunochemotherapy Cell 8/6/2021 [PMID: 34388391]
Anita K. Mehta, Emily M. Cheney, Christina A. Hartl, Constantia Pantelidou, Madisson Oliwa, Jessica A. Castrillon et al. Targeting immunosuppressive macrophages overcomes PARP inhibitor resistance in BRCA1-associated triple-negative breast cancer Nature Cancer 2021-03-19 [PMID: 33738458]
Rumana Rashid, Giorgio Gaglia, Yu-An Chen, Jia-Ren Lin, Ziming Du, Zoltan Maliga et al. Highly multiplexed immunofluorescence images and single-cell data of immune markers in tonsil and lung cancer Scientific Data 2019-12-17 [PMID: 31848351]
Ziming Du, Jia-Ren Lin, Rumana Rashid, Zoltan Maliga, Shu Wang, Jon C. Aster et al. Qualifying antibodies for image-based immune profiling and multiplexed tissue imaging Nature Protocols 2019-10-01 [PMID: 31534232]
Lin J. R, Izar B, et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. Elife 7/11/2018 [PMID: 29993362]

Details:
Citation using the Alexa Fluor 488 format of this antibody.
Show All 13 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD4 Antibody (FAB8165G). (Showing 1 - 1 of 1 FAQs).

  1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
    • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

Secondary Antibodies

 

Isotype Controls

Additional CD4 Products

Blogs on CD4. Showing 1-10 of 23 blog posts - Show all blog posts.

Is Monkeypox Still A Threat?
By Jamshed Arslan, Pharm D, PhD Monkeypox is not deadly like its cousin, smallpox, nor is it as contagious as COVID-19. Yet, it continues to scare the world. In May 2022, a multinational outbreak of a cont...  Read full blog post.

Synthetic Biotic Medicine as Immunotherapy Against Cancer: Evidence From Arginine-Producing Engineered Bacteria
By Jamshed Arslan, Pharm D, PhDWhat do nuts, dairy and red meat have in common? In addition to the fact that they are all edible, one of the answers is L-arginine. This amino acid improves T cell’s respons...  Read full blog post.

Immune Cell Metabolic Flux Influences Type I Diabetes
By Hunter MartinezWhat is Immunometabolism?It is well established that abnormal metabolic environments can be a risk factor for disease development. One characteristic example is the role of dyslipidemia (high lev...  Read full blog post.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.


  Read full blog post.

Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

mTOR Signaling and the Tumor Microenvironment
By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein ...  Read full blog post.

Flow Cytometry Basics for the Non-Expert
By Jody Bonnevier, PhD.Flow cytometry: A Challenging Interdisciplinary ApplicationFlow cytometry has long been the cell analysis method of choice for immunologists, however more and more disciplines have recognize...  Read full blog post.

The role of MHC Class II RT1B and immune response post brain injury
The major histocompatibility complex (MHC) is responsible for binding peptide fragments arising from pathogens in order to display them on the cell surface for recognition from immune cells.  Once recognized, the foreign pathogen is typically evade...  Read full blog post.

Showing 1-10 of 23 blog posts - Show all blog posts.
mFluor Violet Conjugated Antibodies

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Bioinformatics

Gene Symbol CD4
Uniprot