![Immunocytochemistry/Immunofluorescence: BRD4 Antibody [NBP1-86640] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/BRD4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86640-img0008.jpg "Immunocytochemistry/Immunofluorescence: BRD4 Antibody [NBP1-86640] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | Simple Western, ICC/IF, IHC, ChIP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: PQPAKPQQVIQHHHSPRHHKSDPYSTGHLREAPSPLMIHSPQMSQFQSLTHQSPPQQNVQPKKQELRAASVVQPQPLVVVKEEKIHSPIIRSEPFSPSLRPEPPKHPESIKAPVHLPQRPEMKPVDVGRPVIRPPEQNAPPP |
| Predicted Species | Rat (94%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | BRD4 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. IHC-Paraffin HIER pH6 retrieval is recommended. Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for BRD4 Antibody (NBP1-86640)Find related products by research area.
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Epigenetic Control of Autophagy By Christina Towers, PhD. In the last 20 years, epigenetic regulation has become front and center for almost all fields of biology and its role in diseases like cancer and neurodegeneration are being heavily studi... Read full blog post. |
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Understanding the relationship between NUT and BET proteins in NMC NUT has been found to fuse with bromodomain-containing proteins 3 and 4 (BRD3 and BRD4) in NUT midline carcinoma (NMC), a very rare, extremely aggressive, and genetically defined human cancer. NMC has recently been designated as a sub classificati... Read full blog post. |
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NUT - A Protein Coding Gene The NUT gene is found on chromosome 15q14 and encodes for the NUT protein which is a key component of the RNA polymerase II Mediator complex. This multi-subunit assembly is required for all RNA pol II-dependent transcriptional activation, coordinating... Read full blog post. |
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| Gene Symbol | BRD4 |
![Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640] - Staining of human tonsil shows moderate to strong nuclear positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/BRD4-Antibody-Immunohistochemistry-Paraffin-NBP1-86640-img0020.jpg "Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640] - Staining of human tonsil shows moderate to strong nuclear positivity in non-germinal center cells.")
![Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/BRD4-Antibody-Immunohistochemistry-Paraffin-NBP1-86640-img0017.jpg "Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.")
![Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/BRD4-Antibody-Immunohistochemistry-Paraffin-NBP1-86640-img0018.jpg "Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.")
![Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/BRD4-Antibody-Immunohistochemistry-Paraffin-NBP1-86640-img0019.jpg "Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.")
![Simple Western: BRD4 Antibody [NBP1-86640] - Simple Western lane view shows a specific band for BRD4 in 0.2 mg/ml of U-251MG sp lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.](http://images.novusbio.com/fullsize/BRD4-Antibody-Simple-Western-NBP1-86640-img0006.jpg "Simple Western: BRD4 Antibody [NBP1-86640] - Simple Western lane view shows a specific band for BRD4 in 0.2 mg/ml of U-251MG sp lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.")
![Simple Western: BRD4 Antibody [NBP1-86640] - Electropherogram image(s) of corresponding Simple Western lane view. BRD4 antibody was used at 1:60 dilution on U-251MG sp lysates(s).](http://images.novusbio.com/fullsize/BRD4-Antibody-Simple-Western-NBP1-86640-img0007.jpg "Simple Western: BRD4 Antibody [NBP1-86640] - Electropherogram image(s) of corresponding Simple Western lane view. BRD4 antibody was used at 1:60 dilution on U-251MG sp lysates(s).")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")