| Reactivity | HuSpecies Glossary |
| Applications | ELISA |
| Conjugate | HRP |
| Background | The Quantikine Human KLK3/PSA Immunoassay is a 4.5 hour solid phase immunoassay designed to measure KLK3/PSA in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human KLK3/PSA, and antibodies raised against the recombinant protein. Natural human KLK3/PSA showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quant...ikine kit standards, indicating that this kit can be used to determine relative levels of natural human KLK3/PSA. Show More |
| Specificity | Natural and recombinant human KLK3/PSA |
| Source | N/A |
| Assay Type | Solid Phase Sandwich ELISA |
| Inter-Assay | See PDF Datasheet for details |
| Intra-Assay | See PDF Datasheet for details |
| Spike Recovery | See PDF Datasheet for details |
| Sample Volume | See PDF Datasheet for details |
| Gene | KLK3 |
| Dilutions |
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| Application Notes | No significant interference observed with available related molecules. |
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| Publications |
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| Storage | Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
The human tissue kallikrein (KLK) gene family contains 15 members that play important roles in cancer. Notably, kallikrein-1, also known as tissue kallikrein, cleaves kininogen to release the vasoactive kinin peptide, bradykinin or lysyl-bradykinin. Kallikrein-3, called prostate specific antigen (PSA), is an established tumor marker that aids in the diagnosis, staging, and follow up of prostate cancer. Kallikrein-4 is specifically expressed in the prostate and over-expressed in prostate cancer. Kallikrein-5 is widely expressed but found at high levels in skin, breast, brain and testis; over-expression is an indicator of poor prognosis in ovarian cancer. Kallikrein-8 is expressed in the brain and is a novel marker of ovarian and cervical cancer.
Human plasma kallikrein, a serine protease, is synthesized in the liver and circulates in the plasma bound to high molecular weight (HMW) kininogen or as a free zymogen. Once activated by its physiological activator, coagulation factor XII, it displays endopeptidase activity towards peptide bonds after arginine (preferred) and lysine. It cleaves HMW kininogen, its major physiological substrate, to release the potent vasodilator peptide bradykinin. It is also able to cleave a number of inactive precursor proteins to generate active products, such as plasminogen and prourokinase.
| Gene Symbol | KLK3 |
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