| Reactivity | HuSpecies Glossary |
| Applications | WB, Simple Western |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | E. coli-derived recombinant human SGPL1 Lys342-His568 Accession # O95470 |
| Specificity | Detects human SGPL1 in direct ELISAs and Western blots. |
| Source | N/A |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | SGPL1 |
| Purity Statement | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Publications |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Reconstitute at 0.2 mg/mL in sterile PBS. |
SGPL1 (Sphingosine-1-phosphate lyase; also hSPL) is a 60‑63 kDa member of the SGPL1 subfamily, group II decarboxylase family of enzymes. It is very widely expressed, and particularly concentrated in cells undergoing rapid turnover. SGPL1 is found in the ER, and cleaves phosphorylated sphingoid bases into fatty aldehydes and phosphoethanolamine. This regulates a diversity of cellular processes such as apoptosis and T cell migration. Human SGPL1 is 568 amino acids (aa) in length. It is a type III transmembrane protein (luminal N-terminus with no signal sequence) that contains a 507 aa cytoplasmic domain (aa 62‑568). This domain contains a vitamin B6 binding site (aa 344‑364) that is part of a large catalytic region (aa 116‑506). Over aa 342‑568, human SGPL1 shares 86% aa identity with mouse SGPL1.
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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