BRD4 Antibodies
We offer BRD4 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Proximity Ligation Assay, Simple Western, Western Blot. Each BRD4 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our BRD4 Antibodies can be used in a variety of model species: Human, Mouse, Primate, Rat. Use the list below to choose the BRD4 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our BRD4 polyclonal antibodies.
Alternate Names for BRD4 Antibodies
anti-BRD4 antibody, anti-bromodomain containing 4 antibody, anti-bromodomain-containing 4 antibody, anti-CAPchromosome-associated protein antibody, anti-HUNK1bromodomain-containing protein 4 antibody, anti-HUNKI antibody, anti-MCAP antibody, anti-Protein HUNK1 antibody
16 Results for "brd4" in Primary Antibodies
![Western Blot: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Detection of mouse BRD4 by WB of BW5147.3 lysate. Antibody: BRD4 Antibody (BL-149-2H5). Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/BRD4-Antibody-BL-149-2H5-Western-Blot-NBP2-76393-img0008.jpg "Western Blot: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Detection of mouse BRD4 by WB of BW5147.3 lysate. Antibody: BRD4 Antibody (BL-149-2H5). Secondary: HRP-conjugated goat anti-rabbit IgG.")
![Immunocytochemistry/Immunofluorescence: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Antibody: Rabbit anti-BRD4 recombinant monoclonal [BL-149-2H5] (NBP2-76393). Secondary: DyLight 550-conjugated goat anti-rabbit IgG. Counterstain: Phalloidin Alexa Fluor 488-conjugated (green).](http://images.novusbio.com/fullsize/BRD4-Antibody-BL-149-2H5-Immunocytochemistry-Immunofluorescence-NBP2-76393-img0002.jpg)
![Immunohistochemistry-Paraffin: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Section of breast carcinoma. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-149-2H5] used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/BRD4-Antibody-BL-149-2H5-Immunohistochemistry-Paraffin-NBP2-76393-img0011.jpg)
![Flow Cytometry: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Detection of human BRD4 (shaded) in KG-1 cells by flow cytometry. Antibody: BRD4 Antibody (BL-149-2H5) or isotype control (unshaded). Secondary: DyLight(R) 650-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/BRD4-Antibody-BL-149-2H5-Flow-Cytometry-NBP2-76393-img0010.jpg)
![Chromatin Immunoprecipitation Sequencing: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Localization of human BRD4 binding sites in immunoprecipitates from Mia PaCa-2 cells by ChIP-Seq. Antibody: Rabbit anti-BRD4 recombinant monoclonal [BL-149-2H5] NBP2-76393.](http://images.novusbio.com/fullsize/BRD4-Antibody-BL-149-2H5-Chromatin-Immunoprecipitation-Sequencing-NBP2-76393-img0005.jpg)
![Western Blot: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Detection of human BRD4 by WB of HeLa, HEK293T, Jurkat, MDA-MB-231, A-549, SW620, and SK-MEL-28 lysate. Antibody: BRD4 Antibody (BL-149-2H5). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-eEF2.](http://images.novusbio.com/fullsize/BRD4-Antibody-BL-149-2H5-Western-Blot-NBP2-76393-img0007.jpg)
![Immunohistochemistry-Paraffin: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Detection of human BRD4 in FFPE Hela cells by ICC. Antibody: BRD4 Antibody (BL-149-2H5). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/BRD4-Antibody-BL-149-2H5-Immunohistochemistry-Paraffin-NBP2-76393-img0009.jpg)
![Immunoprecipitation: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Detection of human BRD4 by WB of immunoprecipitates from HeLa lysate. Antibodies: Rabbit anti-BRD4 recombinant monoclonal [BL-149-2H5] (NBP2-76393).](http://images.novusbio.com/fullsize/BRD4-Antibody-BL-149-2H5-Immunoprecipitation-NBP2-76393-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: BRD4 Antibody [NBP1-86640] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/BRD4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86640-img0008.jpg "Immunocytochemistry/Immunofluorescence: BRD4 Antibody [NBP1-86640] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640] - Staining of human tonsil shows moderate to strong nuclear positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/BRD4-Antibody-Immunohistochemistry-Paraffin-NBP1-86640-img0020.jpg)
![Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/BRD4-Antibody-Immunohistochemistry-Paraffin-NBP1-86640-img0017.jpg)
![Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/BRD4-Antibody-Immunohistochemistry-Paraffin-NBP1-86640-img0018.jpg)
![Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/BRD4-Antibody-Immunohistochemistry-Paraffin-NBP1-86640-img0019.jpg)
![Simple Western: BRD4 Antibody [NBP1-86640] - Simple Western lane view shows a specific band for BRD4 in 0.2 mg/ml of U-251MG sp lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.](http://images.novusbio.com/fullsize/BRD4-Antibody-Simple-Western-NBP1-86640-img0006.jpg)
![Simple Western: BRD4 Antibody [NBP1-86640] - Electropherogram image(s) of corresponding Simple Western lane view. BRD4 antibody was used at 1:60 dilution on U-251MG sp lysates(s).](http://images.novusbio.com/fullsize/BRD4-Antibody-Simple-Western-NBP1-86640-img0007.jpg)
![Western Blot: BRD4 Antibody (BL-151-6F11) [NBP2-76394] - Whole cell lysate (50 ug) from BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-151-6F11] usedat 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/BRD4-Antibody-BL-151-6F11-Western-Blot-NBP2-76394-img0011.jpg "Western Blot: BRD4 Antibody (BL-151-6F11) [NBP2-76394] - Whole cell lysate (50 ug) from BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-151-6F11] usedat 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds.")
![Immunocytochemistry/Immunofluorescence: BRD4 Antibody (BL-151-6F11) [NBP2-76394] - Detection of human BRD4 (red) in formaldelyde-fixed Hep-G2 cells by ICC-IF. Antibody: Rabbit anti-BRD4 recombinant monoclonal [BL-151-6F11] (NBP2-76394). Secondary: DyLight 594-conjugated goat anti-rabbit IgG. Counterstain: Phalloidin Alexa Fluor 488-conjugated (green) and DAPI (blue).](http://images.novusbio.com/fullsize/BRD4-Antibody-BL-151-6F11-Immunocytochemistry-Immunofluorescence-NBP2-76394-img0003.jpg)
![Immunohistochemistry-Paraffin: BRD4 Antibody (BL-151-6F11) [NBP2-76394] - Rabbit anti-BRD4 recombinant monoclonal [BL-151-6F11]. (Secondary: HRPconjugated goat anti-rabbit IgG Substrate: DAB.](http://images.novusbio.com/fullsize/BRD4-Antibody-BL-151-6F11-Immunohistochemistry-Paraffin-NBP2-76394-img0008.jpg)
![Flow Cytometry: BRD4 Antibody (BL-151-6F11) [NBP2-76394] - Antibody: Rabbit anti-BRD4 recombinant monoclonal [BL-151-6F11] or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/BRD4-Antibody-BL-151-6F11-Flow-Cytometry-NBP2-76394-img0009.jpg)
![Western Blot: BRD4 Antibody (BL-151-6F11) [NBP2-76394] - Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, MDA-MB-231, Hep-G2, A-549, SW620, and SK-MEL-28 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-151-6F11]used at 1:1000.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-GAPDH.](http://images.novusbio.com/fullsize/BRD4-Antibody-BL-151-6F11-Western-Blot-NBP2-76394-img0010.jpg)
![Immunohistochemistry-Paraffin: BRD4 Antibody (BL-151-6F11) [NBP2-76394] - Section of ovarian carcinoma. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-151-6F11] used at 1:50. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG antibody.](http://images.novusbio.com/fullsize/BRD4-Antibody-BL-151-6F11-Immunocytochemistry-Immunofluorescence-NBP2-76394-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: BRD4 Antibody (BL-151-6F11) [NBP2-76394] - Detection of human BRD4 by WB of immunoprecipitates from HeLa lysate. Antibodies: Rabbit anti-BRD4 recombinant monoclonal [BL-3-2F2] (NBP2-76394), rabbit anti-BRD4 recombinant monoclonal [BL-149-2H5] (NBP2-76394), and rabbit anti-BRD4 polyclonal (NBP1-18874).](http://images.novusbio.com/fullsize/BRD4-Antibody-BL-151-6F11-Immunoprecipitation-NBP2-76394-img0006.jpg)
![Western Blot: BRD4 Antibody (CL1115) [NBP2-52958] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-BRD4 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/BRD4-Antibody-CL1115-Western-Blot-NBP2-52958-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: BRD4 Antibody (CL1115) [NBP2-52958] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-BRD4 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Western Blot: BRD4 Antibody (CL1115) [NBP2-52958] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/BRD4-Antibody-CL1115-Western-Blot-NBP2-52958-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: BRD4 Antibody (CL1115) [NBP2-52958] - Staining of MCF-7 cells using the Anti-BRD4 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/BRD4-Antibody-CL1115-Immunocytochemistry-Immunofluorescence-NBP2-52958-img0013.jpg)
![Immunohistochemistry-Paraffin: BRD4 Antibody (CL1115) [NBP2-52958] - Staining of human kidney shows moderate nuclear positivity in cells in renal glomeruli and in tubules.](http://images.novusbio.com/fullsize/BRD4-Antibody-CL1115-Immunohistochemistry-Paraffin-NBP2-52958-img0012.jpg)
![Immunohistochemistry-Paraffin: BRD4 Antibody (CL1115) [NBP2-52958] - Staining of human stomach cancer shows moderate nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/BRD4-Antibody-CL1115-Immunohistochemistry-Paraffin-NBP2-52958-img0009.jpg)
![Immunohistochemistry-Paraffin: BRD4 Antibody (CL1115) [NBP2-52958] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/BRD4-Antibody-CL1115-Immunohistochemistry-Paraffin-NBP2-52958-img0010.jpg)
![Immunohistochemistry-Paraffin: BRD4 Antibody (CL1115) [NBP2-52958] - Staining of human cerebral cortex shows moderate nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize/BRD4-Antibody-CL1115-Immunohistochemistry-Paraffin-NBP2-52958-img0011.jpg)
![Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-18874] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BRD4 used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/BRD4-Antibody-Immunohistochemistry-Paraffin-NBP1-18874-img0034.jpg "Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-18874] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BRD4 used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB")
![Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-18874] - FFPE section of mouse CT26 colon carcinoma. Antibody: Affinity purified rabbit anti-BRD4 used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/BRD4-Antibody-Immunohistochemistry-Paraffin-NBP1-18874-img0033.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: BRD4 Antibody [NBP1-18874] - Detection of human BRD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD4 antibody NBP1-18874 used for IP at 3 ug per reaction. BRD4 was also immunoprecipitated by a different rabbit anti-BRD4 antibody and rabbit anti-BRD4 recombinant monoclonal antibody [BL-149-2H5] (NBP2-76393). For blotting immunoprecipitated BRD4, NBP2-76393 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 1 seconds.](http://images.novusbio.com/fullsize/BRD4-Antibody-Immunoprecipitation-NBP1-18874-img0035.jpg)
![Western Blot: BRD4 Antibody (CL1118) [NBP2-52959] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/BRD4-Antibody-CL1118-Western-Blot-NBP2-52959-img0007.jpg "Western Blot: BRD4 Antibody (CL1118) [NBP2-52959] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG")
![Immunocytochemistry/Immunofluorescence: BRD4 Antibody (CL1118) [NBP2-52959] - Staining of MCF-7 cells using the Anti-BRD4 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/BRD4-Antibody-CL1118-Immunocytochemistry-Immunofluorescence-NBP2-52959-img0013.jpg)
![Immunohistochemistry-Paraffin: BRD4 Antibody (CL1118) [NBP2-52959] - Staining of human kidney shows moderate to strong nuclear positivity in cells in tubules and in glomeruli.](http://images.novusbio.com/fullsize/BRD4-Antibody-CL1118-Immunohistochemistry-Paraffin-NBP2-52959-img0011.jpg)
![Immunohistochemistry-Paraffin: BRD4 Antibody (CL1118) [NBP2-52959] - Staining of human stomach cancer shows moderate to strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/BRD4-Antibody-CL1118-Immunohistochemistry-Paraffin-NBP2-52959-img0008.jpg)
![Immunohistochemistry-Paraffin: BRD4 Antibody (CL1118) [NBP2-52959] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/BRD4-Antibody-CL1118-Immunohistochemistry-Paraffin-NBP2-52959-img0009.jpg)
![Immunohistochemistry-Paraffin: BRD4 Antibody (CL1118) [NBP2-52959] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/BRD4-Antibody-CL1118-Immunohistochemistry-Paraffin-NBP2-52959-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: BRD4 Antibody [NBP2-38931] - Staining of human cell line PC-3 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/BRD4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38931-img0003.jpg "Immunocytochemistry/Immunofluorescence: BRD4 Antibody [NBP2-38931] - Staining of human cell line PC-3 shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: BRD4 Antibody [NBP2-38931] - Staining of human skin shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/BRD4-Antibody-Immunohistochemistry-Paraffin-NBP2-38931-img0006.jpg)
![Immunohistochemistry-Paraffin: BRD4 Antibody [NBP2-38931] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/BRD4-Antibody-Immunohistochemistry-Paraffin-NBP2-38931-img0004.jpg)
![Immunohistochemistry-Paraffin: BRD4 Antibody [NBP2-38931] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/BRD4-Antibody-Immunohistochemistry-Paraffin-NBP2-38931-img0005.jpg)
![Immunohistochemistry-Paraffin: BRD4 Antibody [NBP2-38931] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/BRD4-Antibody-Immunohistochemistry-Paraffin-NBP2-38931-img0007.jpg)
![Flow Cytometry: BRD4 Antibody (RAB-C131) - Chimeric - Azide and BSA Free [NBP3-20125] - HepG2 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG1 version of RAB-C131 (NBP3-20125, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20125_rabbit-brd4-mab-rab-c131-chimeric-azide-and-bsa-free-274202396461.png "Flow Cytometry: BRD4 Antibody (RAB-C131) - Chimeric - Azide and BSA Free [NBP3-20125] - HepG2 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG1 version of RAB-C131 (NBP3-20125, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Immunocytochemistry/Immunofluorescence: BRD4 Antibody (RAB-C131) - Chimeric - Azide and BSA Free [NBP3-20125] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of RAB-C131 (NBP3-20125) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right, top-bottom NBP3-20125, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20125_rabbit-brd4-mab-rab-c131-chimeric-azide-and-bsa-free-2742023924277.png)
![Western Blot: BRD4 Antibody (RAB-C131) - Chimeric - Azide and BSA Free [NBP3-20125] - Nuclear lysate of K562 cells (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RAB-C131 (NBP3-20125) at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20125_rabbit-brd4-mab-rab-c131-chimeric-azide-and-bsa-free-274202392422.png)
![Immunohistochemistry: BRD4 Antibody (1E5) [NBP3-26475] - Image of BRD4 Antibody (1E5) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26475_rabbit-brd4-mab-1e5-26220241535577.jpg "Immunohistochemistry: BRD4 Antibody (1E5) [NBP3-26475] - Image of BRD4 Antibody (1E5) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: BRD4 Antibody (1E5) [NBP3-26475] - Image of BRD4 Antibody (1E5) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26475_rabbit-brd4-mab-1e5-2722024820397.jpg)
![Western Blot: BRD4 Antibody (1E5) [NBP3-26475] - Positive Western Blot detected in: Hela whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate. All lanes: BRD4 Antibody at 1: 1500 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 153, 81, 89 kDa Observed band size: 153 kDa](http://images.novusbio.com/fullsize/nbp3-26475_rabbit-brd4-mab-1e5-282202411485830.jpg)
![Western Blot: BRD4 Antibody [NBP3-15953] - Western blot analysis of extracts of HeLa cells, using BRD4 antibody (NBP3-15953) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/BRD4-Antibody-Western-Blot-NBP3-15953-img0003.jpg "Western Blot: BRD4 Antibody [NBP3-15953] - Western blot analysis of extracts of HeLa cells, using BRD4 antibody (NBP3-15953) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: BRD4 Antibody [NBP3-15953] - Immunohistochemistry of paraffin-embedded human breast cancer using [KO Validated] BRD4 Rabbit pAb (NBP3-15953) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BRD4-Antibody-Immunohistochemistry-Paraffin-NBP3-15953-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: BRD4 Antibody [NBP3-15953] - Western blot analysis of extracts from normal (control) and BRD4 Rabbit pAb knockout (KO) HeLa cells, using BRD4 Rabbit pAb antibody (NBP3-15953) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/BRD4-Antibody-Knockout-Validated-NBP3-15953-img0001.jpg)
![Western Blot: BRD4 Antibody (7L7M8) [NBP3-15452] - Western blot analysis of extracts of various cell lines, using BRD4 Rabbit mAb (NBP3-15452) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/BRD4-Antibody-7L7M8-Western-Blot-NBP3-15452-img0002.jpg "Western Blot: BRD4 Antibody (7L7M8) [NBP3-15452] - Western blot analysis of extracts of various cell lines, using BRD4 Rabbit mAb (NBP3-15452) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: BRD4 Antibody (7L7M8) [NBP3-15452] - Immunohistochemistry of paraffin-embedded human colon using BRD4 Rabbit mAb (NBP3-15452) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BRD4-Antibody-7L7M8-Immunohistochemistry-Paraffin-NBP3-15452-img0001.jpg)
![Western Blot: BRD4 [p Thr204] Antibody (3V5R1) [NBP3-16958] - Western blot analysis of extracts of various cell lines, using (NBP3-16958) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight.C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/BRD4-p-Thr204-Antibody-3V5R1-Western-Blot-NBP3-16958-img0001.jpg "Western Blot: BRD4 [p Thr204] Antibody (3V5R1) [NBP3-16958] - Western blot analysis of extracts of various cell lines, using (NBP3-16958) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight.C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: BRD4 [p Thr204] Antibody (3V5R1) [NBP3-16958] - Western blot analysis of extracts of NIH/3T3 cells, using (NBP3-16958) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) for 30 minutes with NaCl (400mM) after serum-starvation 16-20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/BRD4-p-Thr204-Antibody-3V5R1-Western-Blot-NBP3-16958-img0002.jpg)