alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - Azide and BSA Free

Actin proteins are highly conserved in eukaryotic cells and are involved in cell motility, structure and integrity. Actin and myosin are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Actin filaments, along with microtubules, comprise a major component of the cytoskeleton and play essential roles in regulating cell shape and movement. Actin has been found to be expressed in at least six isoforms, each encoded by a separate gene and expressed in a developmentally regulated and tissue-specific manner. Alpha, beta and gamma actin isoforms have been identified. Alpha actins are a major constituent of the contractile apparatus, whereas beta and gamma actins are involved in the regulation of cell motility.

ACTA2 encodes alpha Smooth Muscle Actin and is also known as alpha-actin, alpha-actin-2, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA). Alpha Smooth Muscle Actin is frequently used as a marker of smooth muscle differentiation and has a theoretical molecular weight of 42 kDa. Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). Excessive accumulation of alpha smooth muscle actin-positive (ACTA2+) activated myofibroblasts is observed in Idiopathic pulmonary fibrosis (2). Expression of smooth muscle alpha actin is regulated by cell proliferation and is altered in pathological conditions including atherosclerosis and is common in metastatic cancers (1,3).

References

1. Chakraborty, R., Saddouk, F. Z., Carrao, A. C., Krause, D. S., Greif, D. M., & Martin, K. A. (2019). Promoters to Study Vascular Smooth Muscle. Arterioscler Thromb Vasc Biol, 39(4), 603-612. doi:10.1161/atvbaha.119.312449

2 .El Agha, E., Moiseenko, A., Kheirollahi, V., De Langhe, S., Crnkovic, S., Kwapiszewska, G., . . . Bellusci, S. (2017). Two-Way Conversion between Lipogenic and Myogenic Fibroblastic Phenotypes Marks the Progression and Resolution of Lung Fibrosis. Cell Stem Cell, 20(2), 261-273.e263. doi:10.1016/j.stem.2016.10.004

3. Chen, Y. C., Gonzalez, M. E., Burman, B., Zhao, X., Anwar, T., Tran, M., . . . Kleer, C. G. (2019). Mesenchymal Stem/Stromal Cell Engulfment Reveals Metastatic Advantage in Breast Cancer. Cell Rep, 27(13), 3916-3926.e3915. doi:10.1016/j.celrep.2019.05.084

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: FLOW, ICC/IF, IF/IHC.

Showing Publications 1 - 10 of 13. Show All 13 Publications. Collapse Publications.

Publications using NBP2-34522	Applications	Species
Dudgeon C, Casabianca A, Harris C et al. Netrin-1 feedforward mechanism promotes pancreatic cancer liver metastasis via hepatic stellate cell activation, retinoid, and ELF3 signaling Cell reports 2023-11-02 [PMID: 37922311] (FLOW, Mouse)	FLOW	Mouse
Lauterbach AL, Wallace RP, Alpar AT et al. Topically-applied collagen-binding serum albumin-fused interleukin-4 modulates wound microenvironment in non-healing wounds NPJ Regenerative medicine 2023-09-11 [PMID: 37696884] (FLOW, Mouse)	FLOW	Mouse
Medzikovic L, Aryan L, Ruffenach G et al. Myocardial fibrosis and calcification are attenuated by microRNA-129-5p targeting Asporin and Sox9 in cardiac fibroblasts JCI insight 2023-05-08 [PMID: 37154157] (ICC/IF, Mouse)	ICC/IF	Mouse
Sulaiman R, De P, Aske JC et al. Characterization and Clinical Relevance of Endometrial CAFs: Correlation between Post-Surgery Event and Resistance to Drugs International journal of molecular sciences 2023-03-29 [PMID: 37047422] (FLOW, Human)	FLOW	Human
Siewiera J, McIntyre TI, Cautivo KM et al. Circumvention of luteolysis reveals parturition pathways in mice dependent upon innate type 2 immunity Immunity 2023-01-24 [PMID: 36750100]
Xie J, Chen H, Hu K et al. CD248-targeted BBIR-T cell therapy against late-activated fibroblasts in cardiac repair after myocardial infarction Research Square 2023-11-21 (FLOW)	FLOW
Yuan T, Xia Y, Pan S et al. STAT6 promoting oxalate crystal deposition-induced renal fibrosis by mediating macrophage-to-myofibroblast transition via inhibiting fatty acid oxidation Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2023-12-01 [PMID: 37924395]
Piera L, Szymanski J, Juszczak M et al. Histamine is involved in the regulation of collagen content in cultured heart myofibroblasts via H(2), H(3) and H(4) histamine receptors Biomed Rep 2021-08-01 [PMID: 34276989] (FLOW) Details: Citation using the Azide and BSA Free format of this antibody.	FLOW
Bloodworth NC, Clark CR, West JD et al. Bone Marrow-Derived Proangiogenic Cells Mediate Pulmonary Arteriole Stiffening via Serotonin 2B Receptor Dependent Mechanism. Circ Res. 2018-12-07 [PMID: 30566041] (IF/IHC, Mouse) Details: Citation used the Alexa Fluor 647 format of this antibody.	IF/IHC	Mouse
Bowler MA, Bersi MR, Ryzhova LM et al. Cadherin-11 as a regulator of valve myofibroblast mechanobiology. Am J Physiol Heart Circ Physiol. 2018-12-01 [PMID: 30359089] (FLOW, Mouse) Details: Citation using the Azide and BSA Free form of this antibody.	FLOW	Mouse
Hirata Y, Aoki H, Shojima T et al. Activation of the AKT Pathway in the Ascending Aorta With Bicuspid Aortic Valve. Circ J. 2018-09-25 [PMID: 30089758] (ICC/IF, ICC/IF, Human)	ICC/IF, ICC/IF	Human
Guzy RD, Li L, Smith C et al. Pulmonary fibrosis requires cell-autonomous mesenchymal fibroblast growth factor (FGF) signaling J Biol Chem. 2017-06-23 [PMID: 28487375] (FLOW, Mouse) Details: Citation used the Alexa Fluor 647 format of this antibody.	FLOW	Mouse
Virk HS Redox-sensitive TRP ion channels in the interaction between human lung myofibroblasts and oxidative stress Thesis 2022-01-01 (ICC/IF, Human)	ICC/IF	Human
Show All 13 Publications. Collapse Publications.

Array NBP2-34522

• alpha-Smooth Muscle Actin Antibodies
• alpha-Smooth Muscle Actin Antibody Pair
• alpha-Smooth Muscle Actin ELISA Kits
• alpha-Smooth Muscle Actin Lysates
• alpha-Smooth Muscle Actin Proteins