Vimentin Antibody

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Vimentin is a type III intermediate filament (IF) protein, encoded by the VIM gene, that plays an essential role in cytoskeletal function and is largely expressed by mesenchymal cell such as endothelial cells, fibroblasts, and immune cells (1). Human vimentin monomer protein is 466 amino acids (aa) in length with a theoretical molecular weight of ~54 kDa (2). Vimentin protein contains a central rod domain composed of four alpha-helices which is flanked by an N-terminal head domain and C-terminal tail domain (1,3). Vimentin monomers dimerize to form more complex structures resulting in the assembly of eight tetramers, referred to as unit-length filaments, which then assemble into full-length intermediate filaments that are 10-12 nm wide (1,3,4).

Activated macrophages have been shown to secrete phosphorylated vimentin which can be stimulated by a variety of pathophysiological factors including oxidized low-density lipoproteins and TNF-alpha or inhibited by IL-10 (1). The vimentin protein is often expressed at the cell surface playing a role in cell-cell interactions, tissue damage and repair, immune response, and pathogen recognition (1). Vimentin functions in many cytoskeletal processes including cell migration, which is highlighted by its upregulation during epithelial-to-mesenchymal transition (EMT) (4,5). Vimentin is a commonly used marker for EMT and is expressed by many tumor types (5). For example, high metastasis of oral squamous cell carcinomas also showed high vimentin positive expression in immunohistochemical staining analysis (5). A number of vimentin targeting compounds are in cancer-related clinical trials, however, given the multifunctional role of vimentin, the effect of inhibition on non-malignant cells needs to be thoroughly examined (5).

References

1. Ramos, I., Stamatakis, K., Oeste, C. L., & Perez-Sala, D. (2020). Vimentin as a Multifaceted Player and Potential Therapeutic Target in Viral Infections. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms21134675

2. Uniprot (P08670)

3. Morrow, C. S., & Moore, D. L. (2020). Vimentin's side gig: Regulating cellular proteostasis in mammalian systems. Cytoskeleton (Hoboken, N.J.). https://doi.org/10.1002/cm.21645

4. van Bodegraven, E. J., & Etienne-Manneville, S. (2020). Intermediate filaments against actomyosin: the david and goliath of cell migration. Current Opinion in Cell Biology. https://doi.org/10.1016/j.ceb.2020.05.006

5. Strouhalova, K., Prechova, M., Gandalovicova, A., Brabek, J., Gregor, M., & Rosel, D. (2020). Vimentin Intermediate Filaments as Potential Target for Cancer Treatment. Cancers. https://doi.org/10.3390/cancers12010184

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Canine, Cynomolgus Monkey.

We have publications tested in 9 applications: B/N, EM, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, KD, WB.

Showing Publications 1 - 10 of 69. Show All 69 Publications. Collapse Publications.

Publications using NB300-223	Applications	Species
Gupta K, Chen D, Wells RG Microcystin-RR is a biliary toxin selective for neonatal cholangiocytes bioRxiv : the preprint server for biology 2023-08-13 [PMID: 37609158] (ICC/IF)	ICC/IF
Ji C TRPV4 Regulates beta 1 Integrin-Mediated Cell-Matrix Adhesions and Collagen Remodeling Thesis 2021-01-01 [PMID: 37219464] (ICC/IF, Mouse)	ICC/IF	Mouse
Feng W, Bais A, He H et al. Single-cell transcriptomic analysis identifies murine heart molecular features at embryonic and neonatal stages Nature communications 2022-12-27 [PMID: 36575170] (IHC, Mouse) Details: Dilution used in IHC 1:500	IHC	Mouse
Ding XI A Filamin A Binding Protein Regulates Vimentin Assembly and Cell Extension Formation Thesis 2020-01-01 (EM, KD, Mouse)	EM, KD	Mouse
Wong EA, Evans S, Kraus CR et al. IL-10 Impairs Local Immune Response in Lung Granulomas and Lymph Nodes during Early Mycobacterium tuberculosis Infection J. Immunol. Feb 1 2020 (IHC, IHC-P, Cynomolgus Monkey)	IHC, IHC-P	Cynomolgus Monkey
Buchel DM Wnt/beta-catenin signaling in malignant mammary tumor progression and metastasis formation & Mechanisms of evasive resistance to sorafenib in hepatocellular carcinoma Thesis (ICC/IF, Human)	ICC/IF	Human
Ihms EA DOWN THE RABBIT HOLE: UNRAVELING THE PATHOGENESIS OF PULMONARY CAVITATION DURING MYCOBACTERIUM TUBERCULOSIS INFECTION Thesis Jan 1 2019 (IHC-P, Mouse)	IHC-P	Mouse
Patteson A, Pogoda K, Byfield F et al. Loss of vimentin intermediate filaments decreases peri-nuclear stiffness and enhances cell motility through confined spaces. arXiv preprint arXiv 2018-07-17
Zrelski MM, Hösele S, Kustermann M et al. Plectin deficiency in fibroblasts deranges intermediate filament and organelle morphology, migration, and adhesion The Journal of investigative dermatology 2023-09-14 [PMID: 37716646]
Dai DL, Li M, Lee EB. Human Alzheimer's disease reactive astrocytes exhibit a loss of homeostastic gene expression Acta Neuropathologica Communications 2023-08-02 [PMID: 37533101] (IHC-P)	IHC-P
Uppal S, Postnikova O, Villasmil R et al. Low-Density Lipoprotein Receptor (LDLR) Is Involved in Internalization of Lentiviral Particles Pseudotyped with SARS-CoV-2 Spike Protein in Ocular Cells International journal of molecular sciences 2023-07-24 [PMID: 37511618] (B/N)	B/N
Orzechowska-Licari EJ, Bialkowska AB, Yang VW Sonic Hedgehog and WNT signaling regulate a positive feedback loop between intestinal epithelial and stromal cells to promote epithelial regeneration Cellular and molecular gastroenterology and hepatology 2023-07-20 [PMID: 37481204] Details: 1:400 WB dilution
Ostrowska-Podhorodecka Z, Ali A, Norouzi M et al. Vimentin-mediated myosin 10 aggregation at tips of cell extensions drives MT1-MMP-dependent collagen degradation in colorectal cancer FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2023-08-01 [PMID: 37440280]
Kálmán M, Oszwald E, Pócsai K Three-plane description of astroglial architecture and gliovascular connections of area postrema in rat: Long tanycyte connections to other parts of brainstem The Journal of comparative neurology 2023-06-01 [PMID: 36994627] (ICC/IF, Rat)	ICC/IF	Rat
Cordova MC. Dissertation Miguel upload version Thesis. 2016-01-01 (ICC/IF, Human)	ICC/IF	Human
Vos S, Aaron R, Weng M et al. CD40 Upregulation in the Retina of Patients With Diabetic Retinopathy: Association With TRAF2/TRAF6 Upregulation and Inflammatory Molecule Expression Investigative ophthalmology & visual science 2023-06-01 [PMID: 37294707] (IHC, Human)	IHC	Human
Takahashi J, Mizutani T, Sugihara H et al. Suspension culture in a rotating bioreactor for efficient generation of human intestinal organoids Cell reports methods 2022-11-21 [PMID: 36452871] (IF/IHC, Human)	IF/IHC	Human
Jassim AH, Nsiah NY, Inman DM. Ocular Hypertension Results in Hypoxia within Glia and Neurons throughout the Visual Projection Antioxidants (Basel) 2022-04-29 [PMID: 35624752]
Chen M, Pekosz A, Villano JS et al. Evolution of nasal and olfactory infection characteristics of SARS-CoV-2 variants bioRxiv : the preprint server for biology 2022-04-12 [PMID: 35441175] (IF/IHC)	IF/IHC
Mecklenburg J, Wangzhou A, Hovhannisyan AH et al. Sex-dependent pain trajectories induced by prolactin require an inflammatory response for pain resolution Brain, behavior, and immunity 2022-01-19 [PMID: 35065194] (IF/IHC, Mouse)	IF/IHC	Mouse
Zhong Y, Zhang ZH, Wang JY et al. Zinc finger protein 91 mediates necroptosis by initiating RIPK1-RIPK3-MLKL signal transduction in response to TNF receptor 1 ligation Toxicology letters 2021-12-20 [PMID: 34942311]
Suprewicz ?, Swoger M, Gupta S et al. Extracellular Vimentin as a Target Against SARS-CoV-2 Host Cell Invasion Small 2022-02-01 [PMID: 34866333]
LUOnd F, Sugiyama N, Bill R Et al. Distinct contributions of partial and full EMT to breast cancer malignancy Developmental cell 2021-11-23 [PMID: 34847378] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Buechel D, Sugiyama N, Rubinstein N Et al. Parsing beta-catenin's cell adhesion and Wnt signaling functions in malignant mammary tumor progression Proceedings of the National Academy of Sciences of the United States of America 2021-08-24 [PMID: 34408016] (ICC/IF, Mouse)	ICC/IF	Mouse
Thillaiappan Nb, Smith Ha, Atakpa-Adaji P, Taylor Cw KRAP tethers IP3 receptors to actin and licenses them to evoke cytosolic Ca2+ signals Nature communications 2021-07-23 [PMID: 34301929]
Portillo JC, Yu JS, Hansen S et al. A cell-penetrating CD40-TRAF2,3 blocking peptide diminishes inflammation and neuronal loss after ischemia/reperfusion FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021-03-01 [PMID: 33675257] (IHC-P, Mouse)	IHC-P	Mouse
Suprewicz Ł, Swoger M, Gupta S, et al. Vimentin binds to SARS-CoV-2 spike protein and antibodies targeting extracellular vimentin block in vitro uptake of SARS-CoV-2 virus-like particles bioRxiv : the preprint server for biology 2021-01-08 [PMID: 33442680] (ICC/IF, Human)	ICC/IF	Human
Ke Y, Fan X, Hao R, et al. Human embryonic stem cell-derived extracellular vesicles alleviate retinal degeneration by upregulating Oct4 to promote retinal Müller cell retrodifferentiation via HSP90 Stem cell research & therapy 2021-01-07 [PMID: 33413616] (ICC/IF)	ICC/IF
Li J, Zhang L, Zheng Y et al. BAD inactivation exacerbates rheumatoid arthritis pathology by promoting survival of sublining macrophages eLife 2020-12-03 [PMID: 33270017] (ICC/IF)	ICC/IF
Baker MJ, Cooke M, Kreider-Letterman G et al. Evaluation of active Rac1 levels in cancer cells: a case of misleading conclusions from immunofluorescence analysis J. Biol. Chem. 2020-08-14 [PMID: 32817335] (ICC/IF, Human)	ICC/IF	Human
Mani V, Lyu Z, Kumar V et al. Epithelial-to-Mesenchymal Transition (EMT) and Drug Response in Dynamic Bioengineered Lung Cancer Microenvironment. Adv Biosyst 2020-07-07 [PMID: 32627339]
Ding I, Ostrowska-Podhorodecka Z, Lee W et al. Cooperative roles of PAK1 and filamin A in regulation of vimentin assembly and cell extension formation Biochim Biophys Acta Mol Cell Res 2020-05-07 [PMID: 32389644]
Calamak S, Ermis M, Sun H et al. A Circulating Bioreactor Reprograms Cancer Cells Toward a More Mesenchymal Niche Adv Biosyst 2020-04-16 [PMID: 32293132]
Zhou X, Wahane S, Friedl M Microglia and macrophages promote corralling, wound compaction and recovery after spinal cord injury via Plexin-B2 Nat. Neurosci. 2020-03-01 [PMID: 32112058] (IHC-P, Mouse)	IHC-P	Mouse
Patteson AE, Pogoda K, Byfield FJ et al. Loss of Vimentin Enhances Cell Motility through Small Confining Spaces Small 2019-11-13 [PMID: 31721440]
Patteson A E, Vahabikashi A et al. Vimentin protects cells against nuclear rupture and DNA damage during migration. J Cell Biol 2019-02-12 [PMID: 31676718] (ICC/IF, Mouse)	ICC/IF	Mouse
ClEment T, Lee JB, Ichkova A et al. Juvenile mild traumatic brain injury elicits distinct spatiotemporal astrocyte responses Glia 2019-10-31 [PMID: 31670865]
Ardalani H, Sengupta S, Harms V et al. 3-D Culture and Endothelial Cells Improve Maturity of Human Pluripotent Stem Cell-Derived Hepatocytes Acta Biomater 2019-07-27 [PMID: 31362140] (ICC/IF, Human)	ICC/IF	Human
Arno B, Galli F et al. TNAP limits TGF-beta-dependent cardiac and skeletal muscle fibrosis by inactivating the SMAD2/3 transcription factors. J Cell Sci 2019-08-08 [PMID: 31289197] (IF/IHC, Mouse)	IF/IHC	Mouse
Casado-Medrano, V;Barrio-Real, L;Wang, A;Cooke, M;Lopez-Haber, C;Kazanietz, MG; Distinctive requirement of PKC epsilon in the control of Rho GTPases in epithelial and mesenchymally transformed lung cancer cells Oncogene 2019-03-28 [PMID: 30923343] (ICC/IF, Human)	ICC/IF	Human
Saxena M, Kalathur RKR, Neutzner M et al. PyMT-1099, a versatile murine cell model for EMT in breast cancer Sci Rep 2018-08-14 [PMID: 30108334] (ICC/IF, Mouse)	ICC/IF	Mouse
Li , Wang C, Li Z et al. Nano-sized Al2O3 particle-induced autophagy reduces osteolysis in aseptic loosening of total hip arthroplasty by negative feedback regulation of RANKL expression in fibroblasts Cell Death Dis 2018-08-12 [PMID: 30082761] (ICC/IF, IF/IHC, Human)	ICC/IF, IF/IHC	Human
Li Q, Ingram L, Kim S, Beharry Z. Paracrine Fibroblast Growth Factor Initiates Oncogenic Synergy with Epithelial FGFR/Src Transformation in Prostate Tumor Progression. Neoplasia. 2018-02-11 [PMID: 29444487] (ICC/IF)	ICC/IF
Hulin A, Anstine LJ, Kim AJ et al. Macrophage Transitions in Heart Valve Development and Myxomatous Valve Disease Arterioscler. Thromb. Vasc. Biol. 2018-01-18 [PMID: 29348122] (Mouse)		Mouse
Tocchio A, Durmus NG, Sridhar K et al. Magnetically Guided Self-Assembly and Coding of 3D Living Architectures Adv. Mater. Weinheim [PMID: 29215164] (ICC/IF, Mouse)	ICC/IF	Mouse
Mao L, Gao W, Chen S et al. Epothilone B impairs functional recovery after spinal cord injury by increasing secretion of macrophage colony-stimulating factor Cell Death Dis 2017-11-02 [PMID: 29095439] (ICC/IF, Rat)	ICC/IF	Rat
Anstine LJ, Horne TE, Horwitz EM, Lincoln J. Contribution of Extra-Cardiac Cells in Murine Heart Valves is Age-Dependent. J Am Heart Assoc 2017-10-20 [PMID: 29054843] (ICC/IF, Mouse)	ICC/IF	Mouse
Lin Yu-Jung, Shyu Woei-Cherng, Chang Chi-Wei et al. Tumor Hypoxia Regulates Forkhead Box C1 to Promote Lung Cancer Progression. Theranostics 2019-03-05 [PMID: 28435461] (WB, Human)	WB	Human
Calibasi Kocal G, GUven S, Foygel K et al. Dynamic Microenvironment Induces Phenotypic Plasticity of Esophageal Cancer Cells Under Flow Sci Rep. 2016-12-01 [PMID: 27910892] (ICC/IF, Human)	ICC/IF	Human
Behrens J, Solga R, Ziemann A et al. Coronin 1C-free primary mouse fibroblasts exhibit robust rearrangements in the orientation of actin filaments, microtubules and intermediate filaments. Eur. J. Cell Biol. 2016-05-04 [PMID: 27178841] (ICC/IF, Mouse)	ICC/IF	Mouse
Nakamura R, Koshiba-Takeuchi K, Tsuchiya M et al. Expression analysis of Baf60c during heart regeneration in axolotls and neonatal mice. Dev. Growth Differ. 2016-05-01 [PMID: 27125315] (ICC/IF, Mouse)	ICC/IF	Mouse
Smith LR, Hammers DW, Sweeney HL, Barton ER. Increased collagen cross-linking is a signature of dystrophin-deficient muscle. Muscle Nerve. 2015-11-30 [PMID: 26616495] (IHC-Fr, Mouse, Human, Canine)	IHC-Fr	Mouse, Human, Canine
Lehoczky JA, Tabin CJ Lgr6 marks nail stem cells and is required for digit tip regeneration Proc Natl Acad Sci U S A. 2015-10-26 [PMID: 26460010] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Ge X, Pettan-Brewer C, Morton J et al. Mitochondrial catalase suppresses naturally occurring lung cancer in old mice. Pathobiol Aging Age Relat Dis 2015-09-24 [PMID: 26400209] (IF/IHC, Mouse)	IF/IHC	Mouse
Wong JK, Chen L, Huang Y et al. Attenuation of Cerebral Ischemic Injury in Smad1 Deficient Mice. PLoS ONE 2015-08-31 [PMID: 26317208] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Morissette Martin P, Maux A, Laterreur V et al. Enhancing repair of full-thickness excisional wounds in a murine model: Impact of tissue-engineered biological dressings featuring human differentiated adipocytes. Acta Biomater. 2015-04-29 [PMID: 25934321] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Pocsai K, Kalman M. Glial and perivascular structures in the subfornical organ: distinguishing the shell and core. J Histochem Cytochem 2015-05-01 [PMID: 25673286]
Marcuzzo S, Bonanno S, Kapetis D et al. Up-regulation of neural and cell cycle-related microRNAs in brain of amyotrophic lateral sclerosis mice at late disease stage Mol Brain. 2015-01-28 [PMID: 25626686] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Phamduong E, Rathore MK, Crews NR et al. Acute and Chronic Mu Opioids Differentially Regulate Thrombospondins 1 and 2 Isoforms in Astrocytes. ACS Chem Neurosci 2013-12-05 [PMID: 24304333] (ICC/IF, Rat)	ICC/IF	Rat
Murray ME, Mendez MG, Janmey PA et al. Substrate Stiffness Regulates Solubility of Cellular Vimentin. Mol Biol Cell. 2013-10-30 [PMID: 24173714] (ICC/IF, WB, Human)	ICC/IF, WB	Human
Stadler SC, Vincent CT, Fedorov VD et al. Dysregulation of PAD4-mediated citrullination of nuclear GSK3beta activates TGF-beta signaling and induces epithelial-to-mesenchymal transition in breast cancer cells. Proc Natl Acad Sci U S A 2013-07-16 [PMID: 23818587] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Busch SE, Moser RD, Gurley KE et al. ARF inhibits the growth and malignant progression of non-small-cell lung carcinoma. Oncogene 2013-06-10 [PMID: 23752194] (IHC-P, Mouse)	IHC-P	Mouse
Muir AB, Lim DM, Benitez AJ et al. Esophageal epithelial and mesenchymal cross-talk leads to features of epithelial to mesenchymal transition in vitro Exp Cell Res 2012-12-10 [PMID: 23237990] (ICC/IF, IF/IHC, Human)	ICC/IF, IF/IHC	Human
Zamanian JL, Xu L, Foo LC, Nouri N, Zhou L, Giffard RG, Barres BA. Genomic analysis of reactive astrogliosis. J Neurosci;32(18):6391-410. 2012-05-02 [PMID: 22553043] (IF/IHC, ICC/IF, Mouse)	IF/IHC, ICC/IF	Mouse
Ohashi Shinya, Natsuizaka Mitsuteru, Wong Gabrielle S et al. Epidermal growth factor receptor and mutant p53 expand an esophageal cellular subpopulation capable of epithelial-to-mesenchymal transition through ZEB transcription factors. Cancer Res 2010-01-01 [PMID: 20424117] (ICC/IF, Human)	ICC/IF	Human
Ponzo M, Lesurf R, Petkiewicz S et al. Met induces mammary tumors with diverse histologies and is associated with poor outcome and human basal breast cancer. Proc Natl Acad Sci U S A 2009-01-01 [PMID: 19617568]
Johnstone, C N et al. Inhibits Breast Cancer Tumorigenicity Promotes CDK9-Mediated Peroxisome Proliferator-Activated Receptor Gamma 1 Phosphorylation. Mol Cell Biol; 28: 687 - 704. [PMID: 17998334] (IF/IHC, Human)	IF/IHC	Human
Okawa T, Michaylira CZ, Kalabis J et al. The functional interplay between EGFR overexpression, hTERT activation, p53 mutation in esophageal epithelial cells with activation of stromal fibroblasts induces tumor development, invasion, differentiation. Genes amp; Dev, 2007; 21(21):2788-803. 2007-11-01 [PMID: 17974918] (WB, Human)	WB	Human
Rankin, E B, Tomaszewski, J E, Haase, V H. Renal Cyst Development in Mice with Conditional Inactivation of the von Hippel-Lindau Tumor Suppressor, . 66, 2576-2583. 2006-01-01 [PMID: 16510575] (IF/IHC, Mouse)	IF/IHC	Mouse
Show All 69 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	IHC-Fr	Mouse	01/30/2020	View Summary Application Immunohistochemistry-Frozen Sample Tested mouse frozen heart,Mouse heart frozen sections Species Mouse Lot 6814 Comments Comments Mouse heart after myocardial infarction Frozen section 4% PFA fixation Primary antibody concentration: 1:200 Secondary antibody concentration: 1:500
Enlarge	5 reviewed by: Verified Customer	ICC	Human	04/22/2019	View Summary Application Immunocytochemistry Sample Tested A7 cells Species Human Lot 6816-4 Comments Comments Cells were fixed with 10% formalin and permeabilized with 0.1% TritonX-100. After blocking, cells were incubated with primary antibody (1:600) at 4℃ O/N followed with Alexa Fluor-488 labeled secondary antibody and counterstained with DAPI and Phalloidin for nuclei and F-actin respectively. Results were imaged with Leica TCS SP5 confocal microscope.
Enlarge	5 reviewed by: Verified Customer	IHC-Fr	Mouse	03/11/2019	View Summary Application Immunohistochemistry-Frozen Sample Tested Mouse brain Species Mouse Lot 6816-4
Enlarge	5 reviewed by: Verified Customer	IHC-Fr	Rat	06/15/2017	View Summary Application Immunohistochemistry-Frozen Sample Tested Rat Kidney Sections (Fresh Frozen) Species Rat Lot 91412
Enlarge	5 reviewed by: Verified Customer	ICC	Rat	06/15/2017	View Summary Application Immunocytochemistry Sample Tested NRK52E Species Rat Lot 91412
Enlarge	5 reviewed by: Verified Customer	ICC	Mouse	06/15/2017	View Summary Application Immunocytochemistry Sample Tested NIH-3T3 mouse embryonic fibroblast cell line Species Mouse Lot 91412

Array NB300-223

• Vimentin Antibodies
• Vimentin ELISA Kits
• Vimentin Lysate
• Vimentin Proteins
• Vimentin Proteins and Enzyme