alpha-Smooth Muscle Actin Antibody (1A4/asm-1) - BSA Free

Actin proteins are highly conserved in eukaryotic cells and are involved in cell motility, structure and integrity. Actin and myosin are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Actin filaments, along with microtubules, comprise a major component of the cytoskeleton and play essential roles in regulating cell shape and movement. Actin has been found to be expressed in at least six isoforms, each encoded by a separate gene and expressed in a developmentally regulated and tissue-specific manner. Alpha, beta and gamma actin isoforms have been identified. Alpha actins are a major constituent of the contractile apparatus, whereas beta and gamma actins are involved in the regulation of cell motility.

ACTA2 encodes alpha Smooth Muscle Actin and is also known as alpha-actin, alpha-actin-2, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA). Alpha Smooth Muscle Actin is frequently used as a marker of smooth muscle differentiation and has a theoretical molecular weight of 42 kDa. Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). Excessive accumulation of alpha smooth muscle actin-positive (ACTA2+) activated myofibroblasts is observed in Idiopathic pulmonary fibrosis (2). Expression of smooth muscle alpha actin is regulated by cell proliferation and is altered in pathological conditions including atherosclerosis and is common in metastatic cancers (1,3).

References

1. Chakraborty, R., Saddouk, F. Z., Carrao, A. C., Krause, D. S., Greif, D. M., & Martin, K. A. (2019). Promoters to Study Vascular Smooth Muscle. Arterioscler Thromb Vasc Biol, 39(4), 603-612. doi:10.1161/atvbaha.119.312449

2 .El Agha, E., Moiseenko, A., Kheirollahi, V., De Langhe, S., Crnkovic, S., Kwapiszewska, G., . . . Bellusci, S. (2017). Two-Way Conversion between Lipogenic and Myogenic Fibroblastic Phenotypes Marks the Progression and Resolution of Lung Fibrosis. Cell Stem Cell, 20(2), 261-273.e263. doi:10.1016/j.stem.2016.10.004

3. Chen, Y. C., Gonzalez, M. E., Burman, B., Zhao, X., Anwar, T., Tran, M., . . . Kleer, C. G. (2019). Mesenchymal Stem/Stromal Cell Engulfment Reveals Metastatic Advantage in Breast Cancer. Cell Rep, 27(13), 3916-3926.e3915. doi:10.1016/j.celrep.2019.05.084

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Feline, Porcine,  Human.

We have publications tested in 7 applications: FLOW, ICC/IF, IF/IHC, IHC-Fr, IHC-P, Simple Western, WB.

Showing Publications 1 - 10 of 51. Show All 51 Publications. Collapse Publications.

Publications using NBP2-33006	Applications	Species
Hiver S, Shimizu-Mizuno N, Ikawa Y et al. Gse1, a component of the CoREST complex, is required for placenta development in the mouse Developmental biology 2023-04-04 [PMID: 37019373] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Liu P, Zhang D, Huang G et al. Laminin alpha 1 as a target for the treatment of epidural fibrosis by regulating fibrotic mechanisms International journal of molecular medicine 2023-01-01 [PMID: 36382649] (IHC-P, Rat) Details: Dilution used in IHC-P 1:200	IHC-P	Rat
Karamched SR Targeted EDTA Chelation Therapy with Albumin Nanoparticles to Reverse Arterial Calcification and Restore Vascular Health in Chronic Kidney Disease Thesis (IF/IHC, WB, Porcine)	IF/IHC, WB	Porcine
Liu F, Wang L, Zhai X et al. A multi-functional double cross-linked chitosan hydrogel with tunable mechanical and antibacterial properties for skin wound dressing Carbohydrate Polymers 2023-12-01 [PMID: 37839832] (IHC-P, Rat)	IHC-P	Rat
Chemaly M, Marlevi D, Iglesias M et al. Biliverdin Reductase B Is a Plasma Biomarker for Intraplaque Hemorrhage and a Predictor of Ischemic Stroke in Patients with Symptomatic Carotid Atherosclerosis Biomolecules 2023-05-24 [PMID: 37371462] (IHC-P, Mouse)	IHC-P	Mouse
Salemi S, Schori L, Gerwinn T et al. Myostatin Overexpression and Smad Pathway in Detrusor Derived from Pediatric Patients with End-Stage Lower Urinary Tract Dysfunction International Journal of Molecular Sciences 2023-02-24 [PMID: 36901894] (IHC-P, Human)	IHC-P	Human
Siewiera J, McIntyre TI, Cautivo KM et al. Circumvention of luteolysis reveals parturition pathways in mice dependent upon innate type 2 immunity Immunity 2023-01-24 [PMID: 36750100]
Goncalves AN, Moura RS, Correia-Pinto J, Nogueira-Silva C Intraluminal chloride regulates lung branching morphogenesis: involvement of PIEZO1/PIEZO2 Respiratory research 2023-02-05 [PMID: 36740669] (WB, Rat)	WB	Rat
Chen JH, Wu PT, Chyau CC et al. The Nephroprotective Effects of Hibiscus sabdariffa Leaf and Ellagic Acid in Vitro and in Vivo Models of Hyperuricemic Nephropathy Journal of agricultural and food chemistry 2022-12-23 [PMID: 36562602]
Xu Y, Lai L, Chen Z et al. Scleral Remolding-Related Gene Expression After Scleral Collagen Cross-Linking Using Ultraviolet A and Riboflavin in Myopic Guinea Pig Model Current eye research 2022-12-15 [PMID: 36519626]
Cicalese S, Torimoto K, Okuno K et al. Endoplasmic Reticulum Chemical Chaperone 3-Hydroxy-2-Naphthoic Acid Reduces Angiotensin II-Induced Vascular Remodeling and Hypertension In Vivo and Protein Synthesis In Vitro Journal of the American Heart Association 2022-11-29 [PMID: 36444851] (IHC-P, Mouse) Details: Dilution: 1:500	IHC-P	Mouse
Liao J, Gan Y, Peng M et al. GDF15 alleviates the progression of benign tracheobronchial stenosis by inhibiting epithelial-mesenchymal transition and inactivating fibroblasts Experimental cell research 2022-12-15 [PMID: 36336027] (ICC/IF, Human)	ICC/IF	Human
Kim DK, Jeong J, Lee DS Et al. PD-L1-directed PlGF/VEGF blockade synergizes with chemotherapy by targeting CD141(+) cancer-associated fibroblasts in pancreatic cancer Nat Commun 2022-10-22 [PMID: 36272973] (FLOW, Mouse) Details: Citation using the HRP version of this antibody.	FLOW	Mouse
Gerwinn T, Salemi S, Schori LJ et al. Improved contractile potential in detrusor microtissues from pediatric patients with end stage lower urinary tract dysfunction Frontiers in Cell and Developmental Biology 2022-10-04 [PMID: 36268506]
Itzhak I, Cohen S, Fishman S, Fishman P A3 adenosine receptor allosteric modulator CF602 reverses erectile dysfunction in a diabetic rat model Andrologia 2022-06-22 [PMID: 35732294]
Varn FS, Johnson KC, Martinek J Et al. Glioma progression is shaped by genetic evolution and microenvironment interactions Cell 2022-06-01 [PMID: 35649412] (IHC-P, Human) Details: Citation using the Alexa Fluor 647 version of this antibody.	IHC-P	Human
Li X, Yong T, Wei Z et al. Reversing insufficient photothermal therapy-induced tumor relapse and metastasis by regulating cancer-associated fibroblasts Nature communications 2022-05-19 [PMID: 35589680] (IF/IHC, WB, Mouse, Human)	IF/IHC, WB	Mouse, Human
Paul PK, Das R, Drow TJ et al. Pancreatic Stellate Cells Prolong Ex Vivo Islet Viability and Function and Improve Engraftment Stem cells translational medicine 2022-04-19 [PMID: 35438788] (WB)	WB
Tang PC, V Roche M, Young Um S et al. Characterization of an induced pluripotent stem cell line (UMi040-A) bearing an auditory neuropathy spectrum disorder-associated variant in TMEM43 Stem cell research 2022-03-21 [PMID: 35364395] (ICC/IF)	ICC/IF
Rauchbach E, Zeigerman H, Abu-Halaka D, Tirosh O Cholesterol Induces Oxidative Stress, Mitochondrial Damage and Death in Hepatic Stellate Cells to Mitigate Liver Fibrosis in Mice Model of NASH Antioxidants (Basel, Switzerland) 2022-03-11 [PMID: 35326188] (IF/IHC, Mouse)	IF/IHC	Mouse
Sartori AM, Salemi S, Hofer AS et al. Early Transcutaneous Tibial Nerve Stimulation Acutely Improves Lower Urinary Tract Function in Spinal Cord Injured Rats Neurotrauma reports 2022-02-14 [PMID: 35211695] (WB, Simple Western, Rat)	WB, Simple Western	Rat
Ishiwata R, Morimoto Y Hyperphosphatemia-induced degradation of transcription factor EB exacerbates vascular calcification Biochimica et biophysica acta. Molecular basis of disease 2021-12-16 [PMID: 34921974] (IHC-P, Rat)	IHC-P	Rat
Torimoto K, Okuno K, Kuroda R Et al. Glucose consumption of vascular cell types in culture; toward optimization of experimental conditions American journal of physiology. Cell physiology 2021-11-24 [PMID: 34817269]
Gerwinn T, Salemi S, Krattiger L Et al. Spheroids of Bladder Smooth Muscle Cells for Bladder Tissue Engineering BioMed research international 2021-11-11 [PMID: 34805410] (Simple Western, Rat)	Simple Western	Rat
Li F, Guo D, Zhi S Et al. Etoposide-induced protein 2.4 ameliorates high glucose-induced epithelial-mesenchymal transition by activating adenosine monophosphate-activated protein kinase pathway in renal tubular cells The international journal of biochemistry & cell biology 2021-11-18 [PMID: 34801707] (WB, IHC-P, Mouse, Human)	WB, IHC-P	Mouse, Human
Koopmans T, van Beijnum H, Roovers EF et al. Ischemic tolerance and cardiac repair in the spiny mouse (Acomys) NPJ Regen Med 2021-11-17 [PMID: 34789755] Details: Citation using the Alexa Fluor 647 format of this antibody.
Nourbakhsh A, Gosstola N, Fernandez-Valle C Et Al. Characterization of UMi031-A-2 inducible pluripotent stem cell line with a neurofibromatosis type 2-associated mutation Stem Cell Research 2021-08-01 [PMID: 34352618]
Piera L, Szymanski J, Juszczak M et al. Histamine is involved in the regulation of collagen content in cultured heart myofibroblasts via H(2), H(3) and H(4) histamine receptors Biomed Rep 2021-08-01 [PMID: 34276989] (FLOW) Details: Citation using the Azide and BSA Free format of this antibody.	FLOW
Dykxhoorn DM, Tong X, Gosstola NC, Liu XZ Derivation of iPSC line UMi029-A bearing a hearing-loss associated variant in the SMPX gene Stem cell research 2021-05-24 [PMID: 34052664]
Park J, Foox J, Hether T et al. Systemic Tissue and Cellular Disruption from SARS-CoV-2 Infection revealed in COVID-19 Autopsies and Spatial Omics Tissue Maps bioRxiv : the preprint server for biology 2021-03-09 [PMID: 33758858] (Human)		Human
Cho HJ, Kim HJ, Lee K et al. Bioengineered Multicellular Liver Microtissues for Modeling Advanced Hepatic Fibrosis Driven Through Non-Alcoholic Fatty Liver Disease Small (Weinheim an der Bergstrasse, Germany) 2021-03-09 [PMID: 33690979]
Karamched SR Targeted EDTA Chelation Therapy with Albumin Nanoparticles to Reverse Arterial Calcification and Restore Vascular Health in Chronic Kidney Disease Rejuvenation Res 2020-12-01 [PMID: 33256549]
Oikawa K, Teixeira LBC, Keikhosravi A et al. Microstructure and resident cell-types of the feline optic nerve head resemble that of humans Exp Eye Res 2020-10-19 [PMID: 33091431] (ICC/IF, Feline)	ICC/IF	Feline
Hreha TN, Collins CA, Daugherty AL et al. TGF beta 1 orchestrates renal fibrosis following Escherichia coli pyelonephritis Physiol Rep 2020-03-01 [PMID: 32227630] (IHC-P, FLOW, Mouse)	IHC-P, FLOW	Mouse
Yang L, Han B, Zhang M et Al. Activation of BK Channels Prevents Hepatic Stellate Cell Activation and Liver Fibrosis Through the Suppression of TGF beta 1/SMAD3 and JAK/STAT3 Profibrotic Signaling Pathways Front Pharmacol 2020-03-06 [PMID: 32210801] (ICC/IF, IF/IHC, Rat)	ICC/IF, IF/IHC	Rat
Li S, Lin Q, Shao X, et al. Gastric cancer-derived mesenchymal stromal cells trigger M2 macrophage polarization that promotes metastasis and EMT in gastric cancer Cell Death Dis 2019-12-04 [PMID: 31801938] (ICC/IF, Mouse)	ICC/IF	Mouse
GrUneboom A, Hawwari I, Weidner D et al. A network of trans-cortical capillaries as mainstay for blood circulation in long bones Nat Metab. 2019-02-01 [PMID: 31620676] (ICC/IF, Human) Details: Citation used the Alexa Fluor 647 format of this antibody.	ICC/IF	Human
Ruffenach G, Umar S, Vaillancourt M et al. Histological hallmarks and role of Slug/PIP axis in pulmonary hypertension secondary to pulmonary fibrosis EMBO Mol Med. 2019-09-01 [PMID: 31468711] (ICC/IF, Human, Rat) Details: Citation used the Alexa Fluor 647 format of this antibody.	ICC/IF	Human, Rat
Tan T, Hu H, Wang H et al Bioinspired lipoproteins-mediated photothermia remodels tumor stroma to improve cancer cell accessibility of second nanoparticles. Nat Commun. 2019-07-25 [PMID: 31346166] (FLOW, Mouse) Details: Citation using the Alexa Fluor 488 version of this antibody.	FLOW	Mouse
Kangawa A, Nishimura T, Nishimura T et al. Spontaneous Age-Related Histopathological Changes in Microminipigs Toxicol Pathol 2019-07-23 [PMID: 31337280]
Chen YC, Gonzalez ME, Burman B et al. Mesenchymal Stem/Stromal Cell Engulfment Reveals Metastatic Advantage in Breast Cancer Cell Rep 2019-06-25 [PMID: 31242423] (IHC-P, Mouse)	IHC-P	Mouse
An, S;Raju, I;Surenkhuu, B;Kwon, JE;Gulati, S;Karaman, M;Pradeep, A;Sinha, S;Mun, C;Jain, S; Neutrophil extracellular traps (NETs) contribute to pathological changes of ocular graft-vs.-host disease (oGVHD) dry eye: Implications for novel biomarkers and therapeutic strategies Ocul Surf 2019-04-06 [PMID: 30965123] (WB, Human)	WB	Human
Bloodworth NC, Clark CR, West JD et al. Bone Marrow-Derived Proangiogenic Cells Mediate Pulmonary Arteriole Stiffening via Serotonin 2B Receptor Dependent Mechanism. Circ Res. 2018-12-07 [PMID: 30566041] (IF/IHC, Mouse) Details: Citation used the Alexa Fluor 647 format of this antibody.	IF/IHC	Mouse
Bowler MA, Bersi MR, Ryzhova LM et al. Cadherin-11 as a regulator of valve myofibroblast mechanobiology. Am J Physiol Heart Circ Physiol. 2018-12-01 [PMID: 30359089] (FLOW, Mouse) Details: Citation using the Azide and BSA Free form of this antibody.	FLOW	Mouse
Hirata Y, Aoki H, Shojima T et al. Activation of the AKT Pathway in the Ascending Aorta With Bicuspid Aortic Valve. Circ J. 2018-09-25 [PMID: 30089758] (ICC/IF, Human)	ICC/IF	Human
Canuto MJM, Yacoub CRD, Trindade MAB, Avancini J. Histoid leprosy: clinical and histopathological analysis of patients in follow-up in University Clinical Hospital of endemic country. Int. J. Dermatol. 2018-01-31 [PMID: 29384191] (Human)		Human
Chen Y, Wu Z, Yuan B et al. MicroRNA-146a-5p attenuates irradiation-induced and LPS-induced hepatic stellate cell activation and hepatocyte apoptosis through inhibition of TLR4 pathway. Cell Death Dis. 2018-01-18 [PMID: 29348414] (ICC/IF, Human)	ICC/IF	Human
Guzy RD, Li L, Smith C et al. Pulmonary fibrosis requires cell-autonomous mesenchymal fibroblast growth factor (FGF) signaling J Biol Chem. 2017-06-23 [PMID: 28487375] (FLOW, Mouse) Details: Citation used the Alexa Fluor 647 format of this antibody.	FLOW	Mouse
El Agha E, Moiseenko A, Kheirollahi V et al. Two-Way Conversion between Lipogenic and Myogenic Fibroblastic Phenotypes Marks the Progression and Resolution of Lung Fibrosis. Cell Stem Cell. 2016-11-14 [PMID: 27867035] Details: This citation used the Alexa Fluor 405 version of this antibody.
Zhu L, Zhao S, Liu S et al. PTEN Regulates Renal Extracellular Matrix Deposit via Increased CTGF in Diabetes Mellitus J Cell Biochem [PMID: 26447680]
Show All 51 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	4 reviewed by: Nick Dordai	WB	Human	12/01/2022	View Summary Application Western Blot Sample Tested MCF-7,MDA-MB-231,SUM159 Species Human Lot IBR092203A-4
	5 reviewed by: Verified Customer	IHC-Fr	Canine	12/16/2019	View Summary Application Immunohistochemistry-Frozen Sample Tested brain and spinal cord Species Canine Lot IBR051902A Comments Comments Alexa Fluor conjugated secondary antibodies were used
	5 reviewed by: Verified Customer	IHC-Fr	Feline	02/07/2017	View Summary Application Immunohistochemistry-Frozen Sample Tested Vascular Smooth Muscle Cells Species Feline Lot N/A Comments Comments Antibody dilution was 1:600

Array NBP2-33006

• alpha-Smooth Muscle Actin Antibodies
• alpha-Smooth Muscle Actin Antibody Pair
• alpha-Smooth Muscle Actin ELISA Kits
• alpha-Smooth Muscle Actin Lysates
• alpha-Smooth Muscle Actin Proteins