Fibronectin Antibody - BSA Free

Fibronectin (FN-EDA, FN, fibronectin 1) is a glycoprotein that is involved in cell adhesion as well as cell migration processes such as embryogenesis, wound healing, host defense and metastasis. Fibronectin appears to be an important factor in the ability of cancerous cells to metastasize by inducing matrix metalloproteinases (MMPs). In a study of human laryngeal carcinoma cells, researchers showed that FN-induced expression of MMP-9 increased the DNA binding activity of transcription factors NF-kappa-B, Activator protein-1 and Specificity protein-1 to the promoter region (PMID: 20638438). Additionally, a study of renal cell cancer showed that the mRNA expression of fibronectin 1 was higher in patients with renal cell cancer as compared to patients with healthy kidneys. This disparity in mRNA expression levels could potentially be used to determine the aggressiveness of renal cell cancer as well as indicate the progression of the disease in patients (PMID: 20860816). Furthermore, a knockdown study of endogenous FN in the A431-III cell line showed a significant decrease of the migratory and invasive activity of the cells as well as an inhibition of secreted MMP-9 (PMID: 21036738).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Bovine, Canine, Equine.

We have publications tested in 7 applications: EM, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, WB.

Showing Publications 1 - 10 of 52. Show All 52 Publications. Collapse Publications.

Publications using NBP1-91258	Applications	Species
Sung MS, Kim SY, Eom GH, Park SW High VEGF Concentrations Accelerate Human Trabecular Meshwork Fibrosis in a TAZ-Dependent Manner International journal of molecular sciences 2023-06-01 [PMID: 37298577] (WB, Human)	WB	Human
Barreto R, Carvalho H, Matias G et al. THE EXTRACELLULAR MATRIX PROTEIN PATTERN IN THE CANINE NEOPLASTIC MAMMARY GLAND Tissue and Cell 2023-02-01 [PMID: 36933273] (IHC, Canine)	IHC	Canine
Wu X, Zhang D, Qiao X et al. Regulating the cell shift of endothelial cell-like myofibroblasts in pulmonary fibrosis The European respiratory journal 2023-06-01 [PMID: 36758986] (WB, Mouse)	WB	Mouse
Li XF, Selli C, Zhou HL et al. Macrophages promote anti-androgen resistance in prostate cancer bone disease The Journal of experimental medicine 2023-04-03 [PMID: 36749798] (IHC-P, ICC/IF, Mouse) Details: Dilution used in IHC-P and ICC 1:500	IHC-P, ICC/IF	Mouse
Jung S, Jiang L, Zhao J et al. Clathrin Light Chain Conjugated Drug Delivery for Cancer Bioengineering & Translational Medicine 2021-11-28 [PMID: 36684105] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Kuczwara V, Schuler G, Pfarrer C et al. Ultrastructural and Immunohistochemical Characterization of Maternal Myofibroblasts in the Bovine Placenta around Parturition Veterinary Sciences 2023-01-07 [PMID: 36669044] (ICC/IF)	ICC/IF
Rüegg AB, Kowalewski MP, Ulbrich SE Endometrial extracellular matrix components do not change over the course of embryonic diapause and reactivation in the roe deer (Capreolus capreolus) Reproduction in domestic animals = Zuchthygiene 2023-05-01 [PMID: 36645739] (IHC)	IHC
Wu M, Matar DY, Yu Z et al. Continuous NPWT Regulates Fibrosis in Murine Diabetic Wound Healing Pharmaceutics 2022-10-06 [PMID: 36297560] (IHC-P, Mouse)	IHC-P	Mouse
Gu L, Li A, Lin J et al. Knockdown of SOX9 alleviates tracheal fibrosis through the Wnt/B-catenin signaling pathway Journal of molecular medicine (Berlin, Germany) 2022-11-01 [PMID: 36192639]
Lee GH, Cheon J, Kim D, Jun HS. Lysophosphatidic Acid Promotes Epithelial-Mesenchymal Transition in Kidney Epithelial Cells via the LPAR1/MAPK-AKT/KLF5 Signaling Pathway in Diabetic Nephropathy International Journal of Molecular Sciences 2022-09-10 [PMID: 36142408] (IHC, WB)	IHC, WB
Passanha FR, Geuens T, LaPointe VLS Cadherin-11 Influences Differentiation in Human Mesenchymal Stem Cells by Regulating the Extracellular Matrix Via the TGF?1 Pathway Stem cells (Dayton, Ohio) 2022-07-27 [PMID: 35416252] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Sterken BA, Ackermann T, MUller C et al. C/EBP beta isoform-specific regulation of migration and invasion in triple-negative breast cancer cells NPJ breast cancer 2022-01-18 [PMID: 35042889] (WB, Human)	WB	Human
Zhang D, Liu B, Jie X et al. Uncovering Bupi Yishen Formula Pharmacological Mechanisms Against Chronic Kidney Disease by Network Pharmacology and Experimental Validation Frontiers in Pharmacology 2021-11-15 [PMID: 34867380]
Carrick FR, Valerio LSA, Gonzalez-Vega MN, Engel D Accelerated Wound Healing Using a Novel Far-Infrared Ceramic Blanket Life 2021-01-01 [PMID: 34575027] (WB, Mouse)	WB	Mouse
Wen S, Deng F, Li L et al. VX-765 ameliorates renal injury and fibrosis in diabetes by regulating caspase-1-mediated pyroptosis and inflammation Journal of diabetes investigation 2021-09-08 [PMID: 34494385] (IHC-P, WB, Mouse, Human)	IHC-P, WB	Mouse, Human
Geuens T, Ruiter FAA, Schumacher A et al. Thiol-ene cross-linked alginate hydrogel encapsulation modulates the extracellular matrix of kidney organoids by reducing abnormal type 1a1 collagen deposition Biomaterials 2021-06-19 [PMID: 34198162] (IF/IHC, Human)	IF/IHC	Human
Hupy ML, Pedler MG, Shieh B et al. Suppression of epithelial to mesenchymal transition markers in mouse lens by a Smad7-based recombinant protein Chemico-Biological Interactions 2021-08-01 [PMID: 33961834] (EM)	EM
Chen X, Li C, Liu J et al. Inhibition of ER stress by targeting the IRE1alpha-TXNDC5 pathway alleviates crystalline silica-induced pulmonary fibrosis International immunopharmacology 2021-03-07 [PMID: 33691254] (IHC-P, Mouse)	IHC-P	Mouse
Jiang L, Jung S, Zhao J et al. Simultaneous targeting of primary tumor, draining lymph node, and distant metastases through high endothelial venule-targeted delivery Nano Today 2021-02-01 [PMID: 33391389] (ICC/IF, Mouse) Details: Immunofluorescence was performed on mouse cancer cells.	ICC/IF	Mouse
Liu Y, Zhang H, Wang S et al. Reduced pericyte and tight junction coverage in old diabetic rats are associated with hyperglycemia-induced cerebrovascular pericyte dysfunction American journal of physiology. Heart and circulatory physiology 2020-12-11 [PMID: 33306445]
Jiang L, Wang YJ, Zhao J et al. Direct Tumor Killing and Immunotherapy through Anti-SerpinB9 Therapy Cell 2020-11-25 [PMID: 33242418]
Roberts AL, Mavlyutov TA, Perlmutter TE et al. Fibronectin extra domain A (FN-EDA) elevates intraocular pressure through Toll-like receptor 4 signaling Sci Rep 2020-06-17 [PMID: 32555351] (IHC-P, Mouse)	IHC-P	Mouse
Sen P, Helmke A, Liao CM et al. SerpinB2 Regulates Immune Response in Kidney Injury and Aging Journal of the American Society of Nephrology 2020-05-01 [PMID: 32209589]
Wen S, Li S, Li L, Fan Q circACTR2: A Novel Mechanism Regulating High Glucose-Induced Fibrosis in Renal Tubular Cells via Pyroptosis Biol. Pharm. Bull. 2020-03-03 [PMID: 32115515] (WB, Human)	WB	Human
Wen S, Wang ZH, Zhang CX et al. Caspase-3 Promotes Diabetic Kidney Disease Through Gasdermin E-Mediated Progression to Secondary Necrosis During Apoptosis Diabetes Metab Syndr Obes 2020-02-10 [PMID: 32104028] (WB, Human, Mouse)	WB	Human, Mouse
Sharma S, Plotkin M Id1 expression in kidney endothelial cells protects against diabetes-induced microvascular injury FEBS Open Bio. [PMID: 31957231] (IF/IHC, ICC/IF, Mouse)	IF/IHC, ICC/IF	Mouse
He X, Chen S, Li C et al. Trehalose Alleviates Crystalline Silica-Induced Pulmonary Fibrosis via Activation of the TFEB-Mediated Autophagy-Lysosomal System in Alveolar Macrophages Cells 2020-01-04 [PMID: 31947943] (IF/IHC, Mouse)	IF/IHC	Mouse
Hernandez H, Roberts AL, McDowell CM Nuclear factor-kappa beta signaling is required for transforming growth factor Beta-2 induced ocular hypertension Exp. Eye Res. 2020-01-08 [PMID: 31923415] (IHC-P, Mouse)	IHC-P	Mouse
Fu C, Lu Y, Williams MA, et al. Emergency myelopoiesis contributes to immune cell exhaustion and pulmonary vascular remodeling Br. J. Pharmacol. 2019-12-03 [PMID: 31793661] (WB, Mouse)	WB	Mouse
Landry NM, Rattan SG, Dixon IMC An Improved Method of Maintaining Primary Murine Cardiac Fibroblasts in Two-Dimensional Cell Culture Sci Rep 2019-09-09 [PMID: 31501457] (WB, Mouse)	WB	Mouse
Barreto RS, Romagnolli P, Fratini P et al. Mouse placental scaffolds: a three-dimensional environment model for recellularization J Tissue Eng 2019-08-08 [PMID: 31448074] (IHC-P, Mouse)	IHC-P	Mouse
Xu L, Sharkey D, Cantley LG Tubular GM-CSF Promotes Late MCP-1/CCR2-Mediated Fibrosis and Inflammation after Ischemia/Reperfusion Injury J. Am. Soc. Nephrol. 2019-07-17 [PMID: 31315923] (WB, Mouse)	WB	Mouse
Borghesi J, da Silva Ferreira H, Favaron PO et al. Characterization of the extracellular matrix in the chorioallantoic membrane of water buffalo (Bubalus bubalis) in early gestation Reprod. Domest. Anim. 2019-07-06 [PMID: 31278783] (IF/IHC)	IF/IHC
Wang P, Wang WJ, Choi-Nurvitadhi J et al. RNA m6A methylation regulates the epithelial mesenchymal transition of cancer cells and translation of Snail Nat Commun 2019-05-06 [PMID: 31061416] (WB, Human)	WB	Human
Zheng, M;Cai, J;Liu, Z;Shu, S;Wang, Y;Tang, C;Dong, Z; Nicotinamide reduces renal interstitial fibrosis by suppressing tubular injury and inflammation J. Cell. Mol. Med. 2019-04-16 [PMID: 30993884] (WB, Mouse)	WB	Mouse
Zha D, Yao T, Bao L et al. Telmisartan attenuates diabetic nephropathy progression by inhibiting the dimerization of angiotensin type-1 receptor and adiponectin receptor-1. Life Sci. 2019-01-27 [PMID: 30699330] (WB, Rat)	WB	Rat
da Palma RK, Fratini P, Schiavo Matias GS et al. Equine lung decellularization: a potential approach for in vitro modeling the role of the extracellular matrix in asthma. J Tissue Eng. 2018-11-12 [PMID: 30450188] (IHC-P, Equine)	IHC-P	Equine
Matias GSS, Rigoglio NN, Carreira ACO et al. Optimization of Canine Placenta Decellularization: An Alternative Source of Biological Scaffolds for Regenerative Medicine. Cells Tissues Organs (Print) 2018-09-10 [PMID: 30199873] (IHC-P, Canine)	IHC-P	Canine
Bao H, Sin TK, Zhang G. Activin A induces leiomyoma cell proliferation, extracellular matrix (ECM) accumulation and myofibroblastic transformation of myometrial cells via p38 MAPK. Biochem. Biophys. Res. Commun. 2018-10-02 [PMID: 30195496] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Eissa LA, Kenawy HI, El-Karef A et al. Antioxidant and anti-inflammatory activities of berberine attenuate hepatic fibrosis induced by thioacetamide injection in rats Chem. Biol. Interact. 2018-08-21 [PMID: 30138605] (IHC-P, Rat)	IHC-P	Rat
Jung MY, Kang JH, Hernandez DM, Yin X. Fatty acid synthase is required for profibrotic TGF-b signaling. FASEB J. 2018-02-16 [PMID: 29475397] (IF/IHC, Mouse)	IF/IHC	Mouse
Graubner FR, Boos A, Aslan S, Kucukaslan I. Uterine and placental distribution of selected extracellular matrix (ECM) components in the dog. Reproduction. 2018-02-09 [PMID: 29439094] (Canine)		Canine
da Silva Nunes Barreto R, Romagnolli P, Mess AM, Miglino MA. Decellularized bovine cotyledons may serve as biological scaffolds with preserved vascular arrangement J Tissue Eng Regen Med. 2017-11-21 [PMID: 29164819] (Bovine)		Bovine
Chiang KC, Yeh CN, Pang JS et al. 1a,25(OH)2D3 Analog, MART-10, Inhibits Neuroendocrine Tumor Cell Metastasis After VEGF-A Stimulation. Anticancer Res. 2017-11-01 [PMID: 29061804] (Human)		Human
El-Mihi KA, Kenawy HI, El-Karef A et al. Naringin attenuates thioacetamide-induced liver fibrosis in rats through modulation of the PI3K/Akt pathway Life Sci. 2017-08-19 [PMID: 28830755] (IF/IHC, Rat)	IF/IHC	Rat
Lin Yu-Jung, Shyu Woei-Cherng, Chang Chi-Wei et al. Tumor Hypoxia Regulates Forkhead Box C1 to Promote Lung Cancer Progression. Theranostics 2019-03-05 [PMID: 28435461] (WB, Human)	WB	Human
Fu M, Song Y, Wen Z et al. Inositol Hexaphosphate and Inositol Inhibit Colorectal Cancer Metastasis to the Liver in BALB/c Mice. Nutrients [PMID: 27187454]
Nitta Kyoko, Shi Sen, Nagai Takako et al. Oral AdministRation of N-Acetyl-seryl-aspartyl-lysyl-proline AmelioRates Kidney Disease in Both Type 1 and Type 2 Diabetic Mice via a Therapeutic Regimen. BioMed Research International 2016-01-01 [PMID: 27088094] (WB, Mouse)	WB	Mouse
Green CJ, Fraser ST, Day ML. Insulin-like growth factor 1 increases apical fibronectin in blastocysts to increase blastocyst attachment to endometrial epithelial cells in vitro. Hum. Reprod. 2015-02-01 [PMID: 25432925] (ICC/IF, Mouse) Details: Fibronectin/Anastellin antibody used for ICC-IF on Day 5 blastocysts that were cultured in serum starved medium for 24 , 48 or 72 h in the absence or presence of IGF1 or in the presence of a PI3K inhibitor LY294002 or in the presence of IGF1, attached to a coverslip - 15 minute 4% paraformaldehyde fixation, 30 minutes permeablization with PBS-Polyvinyl alcohol-0.3% Triton X100, blocking in PPTB (PBS + PVA + 0.1% Tween-20 + 0.7% BSA), ON 4C incubation of primary, anti-rabbit Alexa 488 secondary antibody (Figure 5).	ICC/IF	Mouse
Medina-Ortiz WE, Belmares R, Neubauer S et al. Cellular Fibronectin Expression in Human Trabecular Meshwork and Induction by Transforming Growth Factor-beta2. Invest Ophthalmol Vis Sci. 2013-10-17 [PMID: 24030464]
Hosick PA, Alamodi AA, Storm MV et al. Chronic carbon monoxide treatment attenuates development of obesity and remodels adipocytes in mice fed a high-fat diet. Int J Obes (Lond) 2013-04-30 [PMID: 23689359] (WB, Mouse)	WB	Mouse
Wang J, Jiang ZP, Su N et al. The role of peritoneal alternatively activated macrophages in the process of peritoneal fibrosis related to peritoneal dialysi. Int J Mol Sci 2013-05-17 [PMID: 23685870] (IHC-P, ICC/IF, Mouse)	IHC-P, ICC/IF	Mouse
Show All 52 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	3 reviewed by: Verified Customer	WB	Mouse	10/02/2020	View Summary Application Western Blot Sample Tested Mouse Pancreas Species Mouse Lot N/A
Enlarge	3 reviewed by: Verified Customer	WB	Mouse	10/02/2020	View Summary Application Western Blot Sample Tested Pancreas Species Mouse Lot Lot B2
Enlarge	4 reviewed by: Verified Customer	WB	Human	06/17/2019	View Summary Application Western Blot Sample Tested SVG-A whole cell lysate Species Human Lot D3
Enlarge	4 reviewed by: Kirsten Hattermann-Koch	IHC-Fr	Mouse	05/09/2019	View Summary Application Immunohistochemistry-Frozen Sample Tested Mouse brain Species Mouse Lot D3
Enlarge	5 reviewed by: Verified Customer	IHC-Fr	Pig	12/16/2018	View Summary Application Immunohistochemistry-Frozen Sample Tested trabecular meshwork (TM) region of pig eyes Species Pig Lot D-2
Enlarge	4 reviewed by: Verified Customer	IHC-Fr	Feline	04/27/2018	View Summary Application Immunohistochemistry-Frozen Sample Tested corneal stromal cells Species Feline Lot D-1
Enlarge	5 reviewed by: Verified Customer	IF	Mouse	07/22/2016	View Summary Application Immunofluorescence Sample Tested Mouse Kidneys with Alport Disease Species Mouse Lot A-2
Enlarge	5 reviewed by: Verified Customer	WB	Mouse	04/07/2016	View Summary Application Western Blot Sample Tested Mouse Atherosclerotic Plaque Species Mouse Lot A-4
Enlarge	4 reviewed by: Lindsey Davis	Immunocytochemistry-Frozen	Mouse	06/06/2015	View Summary Application Immunocytochemistry-Frozen Species Mouse
Enlarge	5 reviewed by: Verified Customer	Immunocytochemistry-Paraffin	Human	04/30/2015	View Summary Application Immunocytochemistry-Paraffin Species Human
Enlarge	4 reviewed by: Verified Customer	IHC-P	Mouse	03/02/2015	View Summary Application Immunohistochemistry-Paraffin Sample Tested Mouse kidney Species Mouse Lot A-2

Array NBP1-91258

• Fibronectin Antibodies
• Fibronectin ELISA Kits
• Fibronectin Lysate
• Fibronectin Proteins
• Fibronectin ELISAs
• Fibronectin Proteins and Enzymes