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TGF-beta RI/ALK-5 Antibody [Unconjugated]

 (16 Publications)
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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human TGF-beta RI/ALK-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3025) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for TGF-beta RI/ALK-5 at approximately 50 & 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.2 µg/mL of ...read more
TGF-beta RI/ALK-5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and monensin using Goat Anti-Human TGF-beta RI/ALK-5 Antigen Affinity-purified Polyclonal Antibody ...read more
Myostatin signaling in longissimus dorsi muscle of low-birth-weight (LBWT) and normal-birth-weight (NBWT) neonatal pigs. (A): Representative western blots from four pairs of LBWT and NBWT pigs (N, NBWT; L, LBWT). ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF3025
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF3025-SP
100% Guarantee on every product

TGF-beta RI/ALK-5 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human TGF-beta RI/ALK-5
Leu34-Glu125
Accession # P36897
Specificity
Detects human TGF-beta RI/ALK-5 in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant mouse TGF‑ beta  RI is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TGFBR1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1-0.3 ug/mL
Publications
Read Publications using
AF3025 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGF-beta RI/ALK-5 Antibody [Unconjugated]

  • AAT5
  • activin A receptor type II-like kinase, 53kD
  • activin A receptor type II-like kinase, 53kDa
  • Activin receptor-like kinase 5
  • ACVRLK4
  • ALK-5
  • ALK-5ALK5
  • EC 2.7.11
  • EC 2.7.11.30
  • LDS1A
  • LDS2A
  • Serine/threonine-protein kinase receptor R4
  • SKR4
  • tbetaR-I
  • TGFB1R1
  • TGF-beta receptor type I
  • TGF-beta receptor type-1
  • TGF-beta RI
  • TGF-beta type I receptor
  • TGFbetaRI
  • TGFBR1
  • TGF-bRI
  • TGFR-1
  • transforming growth factor beta receptor I
  • transforming growth factor, beta receptor 1
  • transforming growth factor, beta receptor I (activin A receptor type II-likekinase, 53kD)
  • Transforming growth factor-beta receptor type I

Background

TGF-beta RI, also known as activin-like kinase 5 (ALK-5), is a 55 kDa type I transmembrane serine/threonine receptor kinase. It is ubiquitously expressed and forms a functional heterotetrameric signaling TGF-beta receptor complex with TGF-beta RII. The amino acid sequence of human TGF-beta RI extracellular domain is 91%, 90%, 91%, and 93% identical to that of porcine, mouse, bovine, and rat TGF-beta RI, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TGF-beta RI/ALK-5 Antibody (AF3025)(16)

We have publications tested in 3 confirmed species: Human, Mouse, Bovine.

We have publications tested in 2 applications: ICC, Western Blot.


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ICC
(2)
Western Blot
(10)
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Human
(8)
Mouse
(1)
Bovine
(1)
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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using AF3025 Applications Species
Windus, LCE;Matigian, N;Avery, VM; Induction of Reactive Bone Stromal Fibroblasts in 3D Models of Prostate Cancer Bone Metastases Biology 2023-06-15 [PMID: 37372146] (ICC, Western Blot, Human) ICC, Western Blot Human
H Zhang, B Li, Q Huang, F López-Girá, Y Tanaka, Q Lin, S Mehta, G Wang, M Graham, X Liu, IH Park, A Eichmann, W Min, JH Zhou Mitochondrial dysfunction induces ALK5-SMAD2-mediated hypovascularization and arteriovenous malformations in mouse retinas Nature Communications, 2022-12-10;13(1):7637. 2022-12-10 [PMID: 36496409] (Western Blot, Mouse) Western Blot Mouse
W Cheng, HL Li, SY Xi, XF Zhang, Y Zhu, Le Xing, YX Mo, MM Li, FE Kong, WJ Zhu, XG Chen, HQ Cui, ZM Cao, YF Gong, YQ Tang, Y Zhang, XY Guan, NF Ma, M Liu Growth differentiation factor 1-induced tumour plasticity provides a therapeutic window for immunotherapy in hepatocellular carcinoma Nature Communications, 2021-12-08;12(1):7142. 2021-12-08 [PMID: 34880251] (Western Blot, Human) Western Blot Human
B Nam, H Park, YL Lee, Y Oh, J Park, SY Kim, S Weon, SH Choi, JH Yang, S Jo, TH Kim TGF&beta1 Suppressed Matrix Mineralization of Osteoblasts Differentiation by Regulating SMURF1-C/EBP&beta-DKK1 Axis International Journal of Molecular Sciences, 2020-12-21;21(24):. 2020-12-21 [PMID: 33371439] (Western Blot, Human) Western Blot Human
Chengyu Li, Xueqin Meng, Shuo Liu, Weijian Li, Xue Zhang, Jilong Zhou et al. Oocytes and hypoxanthine orchestrate the G2-M switch mechanism in ovarian granulosa cells Development 7/1/2020 [PMID: 32620578]
Shengjie Wang, Mingliang Huang, Zichen Wang, Wan Wang, Zhiyuan Zhang, Shuting Qu et al. MicroRNA‑133b targets TGF beta receptor I to inhibit TGF‑ beta ‑induced epithelial‑to‑mesenchymal transition and metastasis by suppressing the TGF‑ beta /SMAD pathway in breast cancer International Journal of Oncology 9/18/2019 [PMID: 31545407]
Aurore Devocelle, Lola Lecru, Hélène François, Christophe Desterke, Cindy Gallerne, Pierre Eid et al. Inhibition of TGF-beta 1 Signaling by IL-15: A Novel Role for IL-15 in the Control of Renal Epithelial-Mesenchymal Transition: IL-15 Counteracts TGF-beta 1-Induced EMT in Renal Fibrosis International Journal of Cell Biology 7/7/2019 [PMID: 31360169]
B Wang, X Xu, Z Yang, L Zhang, Y Liu, A Ma, G Xu, M Tang, T Jing, L Wu, Y Liu POH1 contributes to hyperactivation of TGF-? signaling and facilitates hepatocellular carcinoma metastasis through deubiquitinating TGF-? receptors and caveolin-1 EBioMedicine, 2019-02-07;0(0):. 2019-02-07 [PMID: 30745168] (Western Blot, Human) Western Blot Human
Ying Chen, Dan-Dan Wang, Ye-Ping Wu, Dan Su, Tian-Yi Zhou, Ren-Hua Gai, Ying-Ying Fu, Lin Zheng, Qiao-Jun He, Hong Zhu, Bo Yang MDM2 promotes epithelial-mesenchymal transition and metastasis of ovarian cancer SKOV3 cells. British Journal of Cancer, 2017-08-17;0(0):1532-1827. 2017-08-17 [PMID: 28817834] (Western Blot, Human) Western Blot Human
Y Chen, SR McCauley, SE Johnson, RP Rhoads, SW El-Kadi Downregulated Translation Initiation Signaling Predisposes Low-Birth-Weight Neonatal Pigs to Slower Rates of Muscle Protein Synthesis Front Physiol, 2017-07-11;8(0):482. 7/11/2017 [PMID: 28744224]
Show All 16 Publications.

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Bioinformatics

Gene Symbol TGFBR1
Uniprot