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CD31/PECAM-1 Antibody [Unconjugated]

Images

 
Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) ...read more
Mouse splenocytes were stained with Goat Anti-Human/Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628, filled histogram) or control antibody (AB-108-C, open histogram), followed by ...read more
Rat splenocytes were stained with Goat Anti-Human/Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628, filled histogram) or isotype control antibody (AB-108-C, open histogram), ...read more
CD31/PECAM-1 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Human/Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL for 3 hours at ...read more
CD31/PECAM-1 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Goat Anti-Human/Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL overnight ...read more
CD31/PECAM-1 was detected in immersion fixed frozen sections of mouse embryo (14 d.p.c.) using Goat Anti-Human/Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL for 1 ...read more
CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of rat heart using Goat Anti-Human/Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at ...read more
CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of Human Placenta using Goat Anti-Human/Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at ...read more
HUVEC cells were stained with Goat Anti-Human/Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628, filled histogram) or isotype control antibody (Catalog # AB-108-C, open ...read more
Western blot shows lysates of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 0.5 µg/ml of Goat Anti-Human/Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (AF3628) ...read more
Representative image of immunostaining corresponding to the glucose transporter protein SLC2A1 (GLUT1) in 30 week-old Ins2AKITA and WT cerebral cortex. Mural cells (ANPEP, green), glucose transporter 1 (GLUT1, red), ...read more
AcSDKP suppresses TGF beta /smad signaling and EndMT through the FGFR1/FRS2 pathway. (a) HMVECs were treated with N-FGFR1 for 48 h, and the FGFR1, TGF beta R1 and TGF beta R2 protein levels were analyzed by western ...read more
AcSDKP inhibits TGF beta /smad signaling and EndMT and restores the FGFR1 and P-MAP4K4 levels in diabetic hearts. (a) Immunofluorescence microscopy analysis of CD31/FGFR1 and CD31/P-MAP4K4 in the heart tissues from each ...read more
AcSDKP suppresses TGF beta /smad signaling and EndMT through the FGFR1/FRS2 pathway. (a) HMVECs were treated with N-FGFR1 for 48 h, and the FGFR1, TGF beta R1 and TGF beta R2 protein levels were analyzed by western ...read more
AcSDKP inhibits TGF beta /smad signaling and EndMT and restores the FGFR1 and P-MAP4K4 levels in diabetic hearts. (a) Immunofluorescence microscopy analysis of CD31/FGFR1 and CD31/P-MAP4K4 in the heart tissues from each ...read more
Diminished permeability of liver sinusoids in Plvap-deficient mice.Neither by immunohistochemistry with antibodies against CD31 (A) nor by light microscopy of 1 µm semi-thin sections (B, Richardson's stain) obvious ...read more
Blood-brain barrier permeability measurements in male Ins2AKITA and WT littermate controls. (a) Representative stereomicroscope fluorescence images of brains showing 1 kDa Alexa Fluor 555 cadaverine permeability in ...read more
Diminished permeability of liver sinusoids in Plvap-deficient mice.Neither by immunohistochemistry with antibodies against CD31 (A) nor by light microscopy of 1 µm semi-thin sections (B, Richardson's stain) obvious ...read more
AcSDKP inhibits TGF beta /smad signaling and EndMT and restores the FGFR1 and P-MAP4K4 levels in diabetic hearts. (a) Immunofluorescence microscopy analysis of CD31/FGFR1 and CD31/P-MAP4K4 in the heart tissues from each ...read more
CL and low temperature lead to WAT browning, angiogenesis, and an increased number of myofibroblast-like cells. a, b Histological analysis of adipocyte morphology (H&E), adipocytes (PERI), mitochondria (COX4), ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

CD31/PECAM-1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD31/PECAM-1
Glu18-Lys590
Accession # Q08481
Specificity
Detects human and mouse CD31/PECAM-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant porcine CD31 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PECAM1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Western Blot 0.5 ug/mL
Reviewed Applications
Read 8 Reviews rated 5
using
AF3628 in the following applications:

Publications
Read Publications using
AF3628 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD31/PECAM-1 Antibody [Unconjugated]

  • adhesion molecule
  • CD31 antigen
  • CD31
  • CD31/EndoCAM
  • EndoCAM
  • FLJ34100
  • FLJ58394
  • GPIIA'
  • PECA1
  • PECAM1
  • PECAM-1
  • PECAM-1, CD31/EndoCAM
  • platelet endothelial cell adhesion molecule
  • platelet endothelial cell adhesion molecule-1
  • platelet/endothelial cell adhesion molecule

Background

PECAM-1 (Platelet-Endothelial Cell Adhesion Molecule-1), also known as CD31, is a 130 kDa type I transmembrane glycoprotein adhesion molecule in the immunoglobulin superfamily (1, 2). Expression is restricted to cells involved in circulation, especially endothelial cells, platelets, monocytes, neutrophils and lymphocyte subsets. PECAM-1 is concentrated at cell-cell junctions and is required for Transendothelial Migration (TEM) (1-3). The Extracellular Domain (ECD) of PECAM-1 has ten potential N-linked glycosylation sites and six C2-type Ig-like domains, the first of which is critical for adhesion and extravasation (3, 4). The cytoplasmic domain contains Immunoregulatory Tyrosine-based Inhibitory and Switch Motifs (ITIM, ITSM) that mediate both inhibition and activation via phosphotyrosine-mediated engagement of SH2-containing signaling molecules (1, 5). Metalloproteinase-mediated ectodomain shedding occurs during apoptosis (6) but increased serum PECAM-1 ectodomain in HIV and active multiple sclerosis occurs independent of apoptosis (7, 8). In humans, expression of six isoforms with exon deletions in the cytoplasmic domain is tissue- and stage-specific, but full-length PECAM-1 is predominant. A form lacking the ITSM predominates in mouse (9). Mouse PECAM-1 ECD shows 77%, 63%, 63%, 63%, and 61% amino acid (aa) identity with rat, human, canine, porcine, and bovine PECAM-1, respectively. PECAM-1 participates with other adhesion molecules in some functions, but is the critical molecule for TEM. Homotypic PECAM-1 adhesion in trans, combined with cycling of PECAM-1 to and from surface-connected endothelial cell vesicles, leads leukocytes across endothelial tight junctions (3, 10). Homotypic adhesion and signaling functions also strongly suppress mitochondria-dependent apoptosis (11). In platelets, PECAM-1 is necessary for limiting thrombus formation (12) and promoting integrin-mediated clot retraction and platelet spreading (13), but mechanisms for these phenomena are unclear. PECAM-/- mice are deficient in chemokine-mediated chemotaxis (14).

  1. Ilan, N. and J.A. Madri (2003) Curr. Opin. Cell Biol. 15:515.
  2. Xie, Y. and W.A. Muller (1993) Proc. Natl. Acad. Sci. USA 90:5569.
  3. Liao, F. et al. (1997) J. Exp. Med. 185:1349.
  4. Nakada, M.T. et al. (2000) J. Immunol. 164:452.
  5. Chemnitz, J.M. et al. (2004) J. Immunol. 173:945.
  6. Ilan, N. et al. (2001) FASEB J. 15:362.
  7. Eugenin, E.A. et al. (2006) J. Leukoc. Biol. 79:444.
  8. Losy, J. et al. (1999) J. Neuroimmunol. 99:169.
  9. Wang, Y. et al. (2003) Am. J. Physiol. Heart Circ. Physiol. 284:H1008.
  10. Mamdouh, Z. et al. (2003) Nature 421:748.
  11. Gao, C. et al. (2003) Blood 102:169.
  12. Falati, S. et al. (2006) Blood 107:535.
  13. Wee, J.L. and D.E. Jackson (2005) Blood 106:3816.
  14. Wu, Y. et al. (2005) J. Immunol. 175:3484.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD31/PECAM-1 Antibody (AF3628)(467)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Transgenic Mouse, Xenograft.

We have publications tested in 17 applications: Cell Culture, Cell Selection, Flow Cytometry, Functional Assay, ICC, ICC/IF, IF, IF/IHC, IHC, IHC fixed frozen, IHC-Fr, IHC-P, IHC-Pr, IHC/IF, IIHC, Ifs, Western Blot.


Filter By Application
Cell Culture
(2)
Cell Selection
(1)
Flow Cytometry
(5)
Functional Assay
(1)
ICC
(8)
ICC/IF
(2)
IF
(2)
IF/IHC
(1)
IHC
(194)
IHC fixed frozen
(1)
IHC-Fr
(8)
IHC-P
(19)
IHC-Pr
(1)
IHC/IF
(3)
IIHC
(1)
Ifs
(1)
Western Blot
(6)
All Applications
Filter By Species
Human
(9)
Mouse
(197)
Rat
(33)
Transgenic Mouse
(11)
Xenograft
(3)
All Species
Showing Publications 1 - 10 of 467. Show All 467 Publications.
Publications using AF3628 Applications Species
Forrester, EA;Benítez-Angeles, M;Redford, KE;Rosenbaum, T;Abbott, GW;Barrese, V;Dora, K;Albert, AP;Dannesboe, J;Salles-Crawley, I;Jepps, TA;Greenwood, IA; Crucial role for Sodium Hydrogen Exchangers in SGLT2 inhibitor-induced arterial relaxations bioRxiv : the preprint server for biology 2023-12-07 [PMID: 38116028] (IHC, Rat) IHC Rat
Jeong, JY;Lee, HJ;Kim, N;Li, Y;Rah, JC;Oh, WJ; Impaired neuronal activity as a potential factor contributing to the underdeveloped cerebrovasculature in a young Parkinson's disease mouse model Scientific reports 2023-12-18 [PMID: 38114623] (Western Blot, Transgenic Mouse) Western Blot Transgenic Mouse
Park, J;Zhang, H;Kwak, HJ;Gadhe, CG;Kim, Y;Kim, H;Noh, M;Shin, D;Ha, SJ;Kwon, YG; A novel small molecule, CU05-1189, targeting the pleckstrin homology domain of PDK1 suppresses VEGF-mediated angiogenesis and tumor growth by blocking the Akt signaling pathway Frontiers in pharmacology 2023-11-16 [PMID: 38035024] (IHC, Mouse) IHC Mouse
Kaesler, N;Cheng, M;Nagai, J;O'Sullivan, J;Peisker, F;Bindels, EMJ;Babler, A;Moellmann, J;Droste, P;Franciosa, G;Dugourd, A;Saez-Rodriguez, J;Neuss, S;Lehrke, M;Boor, P;Goettsch, C;Olsen, JV;Speer, T;Lu, TS;Lim, K;Floege, J;Denby, L;Costa, I;Kramann, R; Mapping cardiac remodeling in chronic kidney disease Science advances 2023-11-24 [PMID: 38000021] (IHC, Mouse) IHC Mouse
Sweeney, A;Arora, A;Edwards, S;Mallidi, S; Ultrasound-guided Photoacoustic image Annotation Toolkit in MATLAB (PHANTOM) for preclinical applications bioRxiv : the preprint server for biology 2023-11-10 [PMID: 37986998] (IHC, Mouse) IHC Mouse
Wang, G;Li, Z;Lin, P;Zhang, H;Wang, Y;Zhang, T;Wang, H;Li, H;Lin, L;Zhao, Y;Jia, L;Chen, Y;Ji, H;Zhao, W;Fu, Z;Zhong, Z; Knockdown of Smox protects the integrity of the blood-brain barrier through antioxidant effect and Nrf2 pathway activation in stroke International immunopharmacology 2023-11-18 [PMID: 37984250] (IHC, Mouse) IHC Mouse
Hu, W;Zou, L;Yu, N;Wu, Z;Yang, W;Wu, T;Liu, Y;Pu, Y;Jiang, Y;Zhang, J;Zhu, H;Cheng, F;Feng, S; Catalpol rescues LPS-induced cognitive impairment via inhibition of NF-?b-regulated neuroinflammation and up-regulation of TrkB-mediated BDNF secretion in mice Journal of ethnopharmacology 2023-11-04 [PMID: 37926114] (IHC, Mouse) IHC Mouse
Caronni, N;La Terza, F;Vittoria, FM;Barbiera, G;Mezzanzanica, L;Cuzzola, V;Barresi, S;Pellegatta, M;Canevazzi, P;Dunsmore, G;Leonardi, C;Montaldo, E;Lusito, E;Dugnani, E;Citro, A;Ng, MSF;Schiavo Lena, M;Drago, D;Andolfo, A;Brugiapaglia, S;Scagliotti, A;Mortellaro, A;Corbo, V;Liu, Z;Mondino, A;Dellabona, P;Piemonti, L;Taveggia, C;Doglioni, C;Cappello, P;Novelli, F;Iannacone, M;Ng, LG;Ginhoux, F;Crippa, S;Falconi, M;Bonini, C;Naldini, L;Genua, M;Ostuni, R; IL-1?+ macrophages fuel pathogenic inflammation in pancreatic cancer Nature 2023-11-01 [PMID: 37914939] (IHC, Mouse) IHC Mouse
Cater, RJ;Mukherjee, D;Iturbe, EG;Erramilli, SK;Chen, T;Koo, K;Grez, NS;Reckers, A;Kloss, B;Gawda, T;Choy, BC;Zheng, Z;Clarke, OB;Yee, SW;Kossiakoff, AA;Quick, M;Arnold, T;Mancia, F; Structural and molecular basis of choline uptake into the brain by FLVCR2 bioRxiv : the preprint server for biology 2023-10-05 [PMID: 37873173] (IHC, Transgenic Mouse) IHC Transgenic Mouse
Yamamoto, S;Matsui, K;Kinoshita, Y;Hiroshi Sasaki, ;Sekine, H;Saito, Y;Nakayama, Y;Kume, H;Kimura, T;Yokoo, T;Kobayashi, E; Successful reconstruction of the rat ureter by a syngeneic collagen tube with a cardiomyocyte sheet Regenerative therapy 2023-12-01 [PMID: 37868722] (IHC, Rat) IHC Rat
Show All 467 Publications.

Reviews for CD31/PECAM-1 Antibody (AF3628) (8) 58

Average Rating: 5
(Based on 8 reviews)
We have 8 reviews tested in 2 species: Mouse, Mouse and Mouse brain tissue.

Reviews using AF3628:
Filter by Applications
IHC-Fr
(3)
IHC-P
(1)
IHC
(1)
Simple Western
(1)
ICC
(2)
All Applications
Filter by Species
Mouse
(7)
Mouse and Mouse brain tissue
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
IHC-Fr Mouse 04/05/2024
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse sciatic nerve,Sciatic Nerve
SpeciesMouse
LotYZU0122121

Comments

CommentsAF3628 was diluted 1 in 500 and was left on tissue sections overnight at 4 degrees Celsius. Secondary antibody was donkey anti goat conjugated to Alexa 594.
  5
reviewed by:
Kevin Thorburn
IHC-P Mouse 04/06/2023
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedFFPE
SpeciesMouse
LotYZU0122121
Immunohistochemistry CD31/PECAM-1 AF3628
Enlarge
5
reviewed by:
Verified Customer
IHC Mouse and Mouse brain tissue 05/10/2019
View

Summary

ApplicationImmunohistochemistry
Sample TestedP12 brain cortex,P21 brain cortex
SpeciesMouse and Mouse brain tissue
LotYZU0117011

Comments

CommentsBlocking with 10% donkey serum and PBST (.3%TritonX100). Free floating vibratome sections of 80microns and staining for brain clearing purposes where methanol treatment was used, the antibody still worked really good.
Immunohistochemistry-Frozen CD31/PECAM-1 AF3628
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5
reviewed by:
Ricardo Vieira
IHC-Fr Mouse 05/09/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedAdult spinal cord,Embryonic spinal cord
SpeciesMouse
LotYZU00118051
Simple Western CD31/PECAM-1 AF3628
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5
reviewed by:
Verified Customer
Simple Western Mouse 05/25/2018
View

Summary

ApplicationSimple Western
Sample TestedVein Lysate
SpeciesMouse
LotYZU0118011

Comments

CommentsThis run was performed under reducing conditions using the 12-230 kDa separation system in combination with a secondary anti-goat-HRP antibody (biotechne, HAF109, 1:50). Lysis buffer: RIPA, prot. conc.: 0.2 µg/µl. Simple Western lane view shows one specific signal (~135 kDa) in lysates prepared from a mouse vein.

Immunohistochemistry-Frozen CD31/PECAM-1 AF3628
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5
reviewed by:
Verified Customer
IHC-Fr Mouse 04/02/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedBrain
SpeciesMouse
LotYZU0116121

Comments

CommentsCD31/PECAM‑1 was detected in PFA fixed brain sections of adult mouse using Goat Anti-Mouse/PECAM‑1 (#AF3628) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Alexa568-conjugated Donkey anti-Goat
IgG Secondary Antibody and counterstained with DAPI (blue).
Immunocytochemistry CD31/PECAM-1 AF3628
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5
reviewed by:
Jan Martinek
ICC Mouse 05/26/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedMouse Liver Tissue
SpeciesMouse

Comments

CommentsFrozen Tissue
4% PFA fixation
Immunocytochemistry CD31/PECAM-1 AF3628
Enlarge
5
reviewed by:
Jan Martinek
ICC Mouse 05/26/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedMouse Liver Tissue
SpeciesMouse
LotYZU0116121

Comments

CommentsFrozen tissue
Cold Acetone fixation

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CD31/PECAM-1 Antibody (AF3628). (Showing 1 - 1 of 1 FAQs).

  1. Does CD31/PECAM-1 antibody (AF3628) work on Rat Paraffin embedded tissues(FFPE)?
    • For this antibody AF3628, it has been validated for mouse and rat and listed are multiple publications stating it was used for IHC-Paraffin.

Secondary Antibodies

 

Isotype Controls

Additional CD31/PECAM-1 Products

Blogs on CD31/PECAM-1.


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The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

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Recent Reviews

5
5
8
4
0
3
0
2
0
1
0

Verified Customer
04/05/2024
Application: IHC-Fr
Species: Mouse

Kevin Thorburn
04/06/2023
Application: IHC-P
Species: Mouse

Verified Customer
05/10/2019
Application: IHC
Species: Mouse and Mouse brain tissue

Bioinformatics

Gene Symbol PECAM1
Uniprot