alpha-Smooth Muscle Actin Antibody (1A4/asm-1) [Alexa Fluor® 647]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Actin proteins are highly conserved in eukaryotic cells and are involved in cell motility, structure and integrity. Actin and myosin are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Actin filaments, along with microtubules, comprise a major component of the cytoskeleton and play essential roles in regulating cell shape and movement. Actin has been found to be expressed in at least six isoforms, each encoded by a separate gene and expressed in a developmentally regulated and tissue-specific manner. Alpha, beta and gamma actin isoforms have been identified. Alpha actins are a major constituent of the contractile apparatus, whereas beta and gamma actins are involved in the regulation of cell motility.

ACTA2 encodes alpha Smooth Muscle Actin and is also known as alpha-actin, alpha-actin-2, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA). Alpha Smooth Muscle Actin is frequently used as a marker of smooth muscle differentiation and has a theoretical molecular weight of 42 kDa. Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). Excessive accumulation of alpha smooth muscle actin-positive (ACTA2+) activated myofibroblasts is observed in Idiopathic pulmonary fibrosis (2). Expression of smooth muscle alpha actin is regulated by cell proliferation and is altered in pathological conditions including atherosclerosis and is common in metastatic cancers (1,3).

References

1. Chakraborty, R., Saddouk, F. Z., Carrao, A. C., Krause, D. S., Greif, D. M., & Martin, K. A. (2019). Promoters to Study Vascular Smooth Muscle. Arterioscler Thromb Vasc Biol, 39(4), 603-612. doi:10.1161/atvbaha.119.312449

2 .El Agha, E., Moiseenko, A., Kheirollahi, V., De Langhe, S., Crnkovic, S., Kwapiszewska, G., . . . Bellusci, S. (2017). Two-Way Conversion between Lipogenic and Myogenic Fibroblastic Phenotypes Marks the Progression and Resolution of Lung Fibrosis. Cell Stem Cell, 20(2), 261-273.e263. doi:10.1016/j.stem.2016.10.004

3. Chen, Y. C., Gonzalez, M. E., Burman, B., Zhao, X., Anwar, T., Tran, M., . . . Kleer, C. G. (2019). Mesenchymal Stem/Stromal Cell Engulfment Reveals Metastatic Advantage in Breast Cancer. Cell Rep, 27(13), 3916-3926.e3915. doi:10.1016/j.celrep.2019.05.084

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: FLOW, ICC/ICC/IF, ICC/IF, IF/IHC, IHC-P.

Showing Publications 1 - 10 of 10.

Publications using NBP2-34522AF647	Applications	Species
Medzikovic L, Aryan L, Ruffenach G et al. Myocardial fibrosis and calcification are attenuated by microRNA-129-5p targeting Asporin and Sox9 in cardiac fibroblasts JCI insight 2023-05-08 [PMID: 37154157]
Kim DK, Jeong J, Lee DS Et al. PD-L1-directed PlGF/VEGF blockade synergizes with chemotherapy by targeting CD141(+) cancer-associated fibroblasts in pancreatic cancer Nat Commun 2022-10-22 [PMID: 36272973] (FLOW, Mouse) Details: Citation using the HRP version of this antibody.	FLOW	Mouse
Varn FS, Johnson KC, Martinek J Et al. Glioma progression is shaped by genetic evolution and microenvironment interactions Cell 2022-06-01 [PMID: 35649412] (IHC-P, Human) Details: Citation using the Alexa Fluor 647 version of this antibody.	IHC-P	Human
Koopmans T, van Beijnum H, Roovers EF et al. Ischemic tolerance and cardiac repair in the spiny mouse (Acomys) NPJ Regen Med 2021-11-17 [PMID: 34789755] Details: Citation using the Alexa Fluor 647 format of this antibody.
GrUneboom A, Hawwari I, Weidner D et al. A network of trans-cortical capillaries as mainstay for blood circulation in long bones Nat Metab. 2019-02-01 [PMID: 31620676] (ICC/IF, Human) Details: Citation used the Alexa Fluor 647 format of this antibody.	ICC/IF	Human
Ruffenach G, Umar S, Vaillancourt M et al. Histological hallmarks and role of Slug/PIP axis in pulmonary hypertension secondary to pulmonary fibrosis EMBO Mol Med. 2019-09-01 [PMID: 31468711] (ICC/ICC/IF, Human, Rat) Details: Citation used the Alexa Fluor 647 format of this antibody.	ICC/ICC/IF	Human, Rat
Bloodworth NC, Clark CR, West JD et al. Bone Marrow-Derived Proangiogenic Cells Mediate Pulmonary Arteriole Stiffening via Serotonin 2B Receptor Dependent Mechanism. Circ Res. 2018-12-07 [PMID: 30566041] (IF/IHC, Mouse) Details: Citation used the Alexa Fluor 647 format of this antibody.	IF/IHC	Mouse
Bowler MA, Bersi MR, Ryzhova LM et al. Cadherin-11 as a regulator of valve myofibroblast mechanobiology. Am J Physiol Heart Circ Physiol. 2018-12-01 [PMID: 30359089] Details: Citation using the Azide and BSA Free form of this antibody.
Hirata Y, Aoki H, Shojima T et al. Activation of the AKT Pathway in the Ascending Aorta With Bicuspid Aortic Valve. Circ J. 2018-09-25 [PMID: 30089758] (ICC/IF, Human)	ICC/IF	Human
Guzy RD, Li L, Smith C et al. Pulmonary fibrosis requires cell-autonomous mesenchymal fibroblast growth factor (FGF) signaling J Biol Chem. 2017-06-23 [PMID: 28487375] (FLOW, Mouse) Details: Citation used the Alexa Fluor 647 format of this antibody.	FLOW	Mouse

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Shinford Sun	Flow	Mouse	09/24/2024	View Summary Application Flow Cytometry Sample Tested Human lung cancer cell lines,Lung cancer tissue Species Mouse Lot D171517

Array NBP2-34522AF647

• alpha-Smooth Muscle Actin Antibodies
• alpha-Smooth Muscle Actin Antibody Pair
• alpha-Smooth Muscle Actin ELISA Kits
• alpha-Smooth Muscle Actin Lysates
• alpha-Smooth Muscle Actin Proteins