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STEP Antibody (23E5) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
23E5
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB300-202AF350
Alexa Fluor 405
NB300-202AF405
Alexa Fluor 488
NB300-202AF488
Alexa Fluor 532
NB300-202AF532
Alexa Fluor 594
NB300-202AF594
Alexa Fluor 647
NB300-202AF647
Alexa Fluor 700
NB300-202AF700
Alexa Fluor 750
NB300-202AF750
Allophycocyanin
NB300-202APC
Biotin
NB300-202B
CoraFluor 1
NB300-202CL1
DyLight 350
NB300-202UV
DyLight 405
NB300-202V
DyLight 488
NB300-202G
DyLight 550
NB300-202R
DyLight 594
NB300-202DL594
DyLight 650
NB300-202C
DyLight 680
NB300-202FR
DyLight 755
NB300-202IR
FITC
NB300-202F
HRP
NB300-202H
Janelia Fluor 525
NB300-202JF525
Janelia Fluor 585
NB300-202JF585
Janelia Fluor 635
NB300-202JF635
Janelia Fluor 646
NB300-202JF646
Janelia Fluor 669
NB300-202JF669
PE
NB300-202PE
PerCP
NB300-202PCP
Unconjugated
NB300-202
mFluor Violet 450 SE
NB300-202MFV450
mFluor Violet 500 SE
NB300-202MFV500
mFluor Violet 610 SE
NB300-202MFV610
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STEP Antibody (23E5) [Janelia Fluor® 549] Summary

Immunogen
A synthetic peptide of rat STEP46 (amino acids 57-74: MCTPGCNEEGFGYLVSPR)
Localization
Cytoplasmic
Specificity
NB300-202 is specific for STEP61, STEP46, STEP38, and STEP20.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PTPN5
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for STEP Antibody (23E5) [Janelia Fluor® 549]

  • EC 3.1.3.48
  • FLJ14427
  • Neural-specific protein-tyrosine phosphatase
  • protein tyrosine phosphatase, non-receptor type 5 (striatum-enriched)
  • protein-tyrosine phosphatase striatum-enriched
  • PTPN5
  • STEP
  • STEPPTPSTEP
  • Striatum-enriched protein-tyrosine phosphatase
  • tyrosine-protein phosphatase non-receptor type 5

Background

The protein tyrosine phosphatase non-receptor type 5 or striatum-enriched protein-tyrosine phosphatase (STEP) protein is part of a family of protein tyrosine phosphatases enriched within the CNS, and is a key component of the NMDAR complex. It is expressed within dopaminoceptive neurons in the CNS and particularly enriched within the basal ganglia and related structures. STEP61 is the membrane-associated isoform and STEP46 is the cytosolic protein. In response to transient hypoxia-ischemia in the brain, STEP61 is proteolytically cleaved by calpain, thereby releasing a smaller isoform, STEP33.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB300-202JF549
STEP Antibody (23E5) [Janelia Fluor® 549]
Species: Hu, Mu, Rt
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Bioinformatics

Gene Symbol PTPN5