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GRAP2 Antibody (OTI1G2)

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Western Blot: GRAP2 Antibody (1G2) [NBP2-00764] Analysis of extracts (35ug) from 9 different cell lines by using anti-GRAP2 monoclonal antibody.
Western Blot: GRAP2 Antibody (1G2) [NBP2-00764] Analysis of extracts (35ug) from 9 different cell lines by using anti-GRAP2 monoclonal antibody.
Immunohistochemistry-Paraffin: GRAP2 Antibody (1G2) [NBP2-00764] - Staining of paraffin-embedded Human endometrium tissue using anti-GRAP2 mouse monoclonal antibody.
Flow Cytometry: GRAP2 Antibody (1G2) [NBP2-00764] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GRAP2 antibody, and then analyzed ...read more
Western Blot: GRAP2 Antibody (1G2) [NBP2-00764] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRAP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: GRAP2 Antibody (1G2) [NBP2-00764] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-GRAP2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GRAP2 Antibody (1G2) [NBP2-00764] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GRAP2 mouse monoclonal antibody.
Flow Cytometry: GRAP2 Antibody (1G2) [NBP2-00764] - Analysis of Jurkat cells, using anti-GRAP2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, IHC
Clone
OTI1G2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71457AF350
Alexa Fluor 405
NBP2-71457AF405
Alexa Fluor 488
NBP2-71457AF488
Alexa Fluor 532
NBP2-71457AF532
Alexa Fluor 594
NBP2-71457AF594
Alexa Fluor 647
NBP2-71457AF647
Alexa Fluor 700
NBP2-71457AF700
Alexa Fluor 750
NBP2-71457AF750
Allophycocyanin
NBP2-71457APC
Allophycocyanin/Cy7
NBP2-71457APCCY7
Biotin
NBP2-71457B
DyLight 350
NBP2-71457UV
DyLight 405
NBP2-71457V
DyLight 488
NBP2-71457G
DyLight 550
NBP2-71457R
DyLight 594
NBP2-71457DL594
DyLight 650
NBP2-71457C
DyLight 680
NBP2-71457FR
DyLight 755
NBP2-71457IR
FITC
NBP2-71457F
HRP
NBP2-71457H
Janelia Fluor 549
NBP2-71457JF549
Janelia Fluor 646
NBP2-71457JF646
PE
NBP2-71457PE
PE/Atto594
NBP2-71457PEATT594
PE/Cy5.5
NBP2-71457PECY55
PE/Cy7
NBP2-71457PECY7
PerCP
NBP2-71457PCP
mFluor Violet 450 SE
NBP2-71457MFV450
mFluor Violet 500 SE
NBP2-71457MFV500
mFluor Violet 610 SE
NBP2-71457MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-71457
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GRAP2 Antibody (OTI1G2) Summary

Immunogen
Full length human recombinant protein of human GRAP2(NP_004801) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GRAP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
37.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GRAP2 Antibody (OTI1G2)

  • Adapter protein GRID
  • GADS
  • GADSSH3-SH2-SH3 adapter Mona
  • GRAP2
  • GRB2L
  • GRB-2-like protein
  • GRB2-related adapter protein 2
  • GRB2-related adaptor protein 2
  • GRB2-related protein with insert domain
  • GRBLG
  • GRBLGGRAP-2
  • GRBX
  • Grf40 adapter protein
  • Grf40
  • grf-40
  • GRID
  • GRIDGRPL
  • Growth factor receptor-binding protein
  • growth factor receptor-bound protein 2-related adaptor protein 2
  • GRPL
  • Hematopoietic cell-associated adapter protein GrpL
  • hematopoietic cell-associated adaptor protein GRPL
  • Mona
  • P38
  • Protein GADS
  • SH3-SH2-SH3 adaptor molecule

Background

This gene encodes a member of the GRB2/Sem5/Drk family. This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member, GRB2-related adaptor protein (GRAP), this protein contains an SH2 domain flanked by two SH3 domains. This protein interacts with other proteins, such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2), through its SH3 domains. Transcript variants utilizing alternative polyA sites exist. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GRAP2
Entrez