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p38 gamma/SAPK3 Antibody [Unconjugated]

 (8 Publications)
 
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Western blot shows lysates of PC-12 rat adrenal pheochromocytoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat p38 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1347) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for p38 gamma was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of PC-12 rat adrenal pheochromocytoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat p38 gamma ...read more
p38 gamma was detected in immersion fixed MCF-7 human breast cancer cell line using Rabbit Anti-Human/Mouse/Rat p38 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1347) at 3 µg/mL for 3 hours at room ...read more
Western blot shows recombinant human p38 beta , p38 gamma, p38d and p38a (1 ng/lane). PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat p38 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog ...read more
Simple Western lane view shows lysates of C2C12 mouse myoblast cell line and PC‑12 rat adrenal pheochromocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for p38 gamma at approximately 48 kDa (as ...read more
MAPK expression in normal and diabetic rat inferior vena cava. Immunoblot analyses indicating protein content of A) p44/42-MAPK, B) p38-MAPK, and C) JNK-MAPK in venae cavae from non-diabetic lean Zucker (LNZ) and ...read more
MAPK expression in normal and diabetic rat inferior vena cava. Immunoblot analyses indicating protein content of A) p44/42-MAPK, B) p38-MAPK, and C) JNK-MAPK in venae cavae from non-diabetic lean Zucker (LNZ) and ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF1347-SP
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p38 gamma/SAPK3 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human p38 gamma
Accession # P53778
Specificity
Detects human, mouse, and rat p38 gamma in Western blots. The antibody is highly specific for p38 gamma and in Western blots, shows extremely weak cross-reactivity with recombinant p38 alpha , p38 beta , or p38δ after prolonged exposure to film.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAPK12
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 3-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 2 ug/mL
  • Western Blot 0.2 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
AF1347 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for p38 gamma/SAPK3 Antibody [Unconjugated]

  • EC 2.7.11
  • ERK3
  • ERK-6
  • ERK6MAPK 12
  • Extracellular signal-regulated kinase 6
  • MAP kinase 12
  • MAP kinase p38 gamma
  • MAPK12
  • mitogen-activated protein kinase 12
  • mitogen-activated protein kinase 3
  • Mitogen-activated protein kinase p38 gamma
  • p38 gamma
  • p38gamma
  • PRKM12
  • SAPK-3
  • SAPK3EC 2.7.11.24
  • Stress-activated protein kinase 3

Background

p38 mitogen-activated protein kinase (MAPK)gamma, also known as p38 gamma , MAPK12, SAPK3 and ERK6 is a serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays a role in myoblast differentiation and also in the
down-regulation of cyclin D1 in response to hypoxia in adrenal cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for p38 gamma/SAPK3 Antibody (AF1347)(8)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.


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IHC
(3)
IHC-Fr
(1)
IHC-P
(1)
Immunoprecipitation
(1)
Western Blot
(4)
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Human
(4)
Mouse
(4)
Rat
(1)
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Showing Publications 1 - 8 of 8.
Publications using AF1347 Applications Species
N Morey, M Przybyla, J van der Ho, YD Ke, F Delerue, J van Eersel, LM Ittner Treatment of epilepsy using a targeted p38gamma kinase gene therapy Science Advances, 2022-12-02;8(48):eadd2577. 2022-12-02 [PMID: 36459557] (Western Blot, Mouse) Western Blot Mouse
M Iba, C Kim, J Florio, M Mante, A Adame, E Rockenstei, S Kwon, R Rissman, E Masliah Role of Alterations in Protein Kinase p38&gamma in the Pathogenesis of the Synaptic Pathology in Dementia With Lewy Bodies and &alpha-Synuclein Transgenic Models Front Neurosci, 2020-03-31;14(0):286. 2020-03-31 [PMID: 32296304] (IHC, Human, Mouse) IHC Human, Mouse
H Chen, X Wang, F Guo, P Li, D Peng, J He Impact of p38? Mitogen-Activated Protein Kinase (MAPK) on MDA-MB-231 Breast Cancer Cells Using Metabolomic Approach Int. J. Biochem. Cell Biol., 2018-11-14;0(0):. 2018-11-14 [PMID: 30447427] (IHC-P, Human) IHC-P Human
Potential Neuroprotective Effects of an LSD1 Inhibitor in Retinal Ganglion Cells via p38 MAPK Activity Invest. Ophthalmol. Vis. Sci, 2016-11-01;57(14):6461-6473. 2016-11-01 [PMID: 27893888] (IHC-Fr, Rat) IHC-Fr Rat
R Noseda, M Guerrero-V, V Alberizzi, SC Previtali, DL Sherman, M Palmisano, RL Huganir, KA Nave, A Cuenda, ML Feltri, PJ Brophy, A Bolino Kif13b Regulates PNS and CNS Myelination through the Dlg1 Scaffold PLoS Biol, 2016-04-12;14(4):e1002440. 2016-04-12 [PMID: 27070899] (Western Blot, Mouse) Western Blot Mouse
A J Browne, A Göbel, S Thiele, L C Hofbauer, M Rauner, T D Rachner p38 MAPK regulates the Wnt inhibitor Dickkopf-1 in osteotropic prostate cancer cells Cell Death & Disease 2/25/2016 [PMID: 26913608]
Qi X, Yin N, Ma S, Lepp A, Tang J, Jing W, Johnson B, Dwinell M, Chitambar C, Chen G p38gamma MAPK Is a Therapeutic Target for Triple-Negative Breast Cancer by Stimulation of Cancer Stem-Like Cell Expansion. Stem Cells, 2015-06-23;33(9):2738-47. 2015-06-23 [PMID: 26077647] (IHC, Western Blot, Human, Mouse) IHC, Western Blot Human, Mouse
Korb A, Tohidast-Akrad M, Cetin E, Axmann R, Smolen J, Schett G Differential tissue expression and activation of p38 MAPK alpha, beta, gamma, and delta isoforms in rheumatoid arthritis. Arthritis Rheum., 2006-09-01;54(9):2745-56. 2006-09-01 [PMID: 16947383] (IHC, Immunoprecipitation, Western Blot, Human) IHC, Immunoprecipitation, Western Blot Human

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Bioinformatics

Gene Symbol MAPK12
Uniprot