![Western Blot: NMDAR2B Antibody [NB300-106] - The blots show the examples of immunobands against NMDAR2B (C) and beta-actin antibodies on the membrane, which was performed on the membrane protein prepared from the lumbar spinal cords of the offspring (P14) rats in different groups. Image collected and cropped by CiteAb from the following publication (//www.jbiomedsci.com/content/18/1/64) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-Western-Blot-NB300-106-img0006.jpg "Western Blot: NMDAR2B Antibody [NB300-106] - The blots show the examples of immunobands against NMDAR2B (C) and beta-actin antibodies on the membrane, which was performed on the membrane protein prepared from the lumbar spinal cords of the offspring (P14) rats in different groups. Image collected and cropped by CiteAb from the following publication (//www.jbiomedsci.com/content/18/1/64) licensed under a CC-BY license.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | PLEASE NOTE: If 0.01mg size is ordered, product will come lyophilized from 5 mM ammonium bicarbonate. Please reconstitute lyophilized product in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) |
| Immunogen | Fusion protein from the C-terminal region of the NR2B subunit. Accession # Q00960 |
| Localization | NB300-106 can be used to demonstrate that NR2B is highly enriched in hippocampus compared to either cortex or cerebellum. |
| Specificity | Specific for the ~180kDa NR2B subunit of the NMDA receptor. Recognizes human, mouse and rat forms of the NR2B subunits of NMDAR. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody. No reactivity towards the NR2A and NR2C subunits. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | GRIN2B |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | NB300-106 can be used in Western blot where a band is seen at ~ 180 kDa representing the NMDAR2B. Immunohistochemical analysis can be performed using frozen or fixed tissue sections. NB300-106 can also be used to quantitatively immunoprecipitate NMDAR2B where 3 ul will (under appropriate conditions) quantitatively immunoprecipitate all NMDAR2B in 200 mg of rat brain. NB300-106 has also been used successfully in immunofluoresence as reported in the literature (PMID: 20082293) and reported by an anonymous customer review. After reconstitution the antibody should be aliquoted and stored at -20C. Adequate amount of material to conduct 10-mini Western Blots. |
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| Theoretical MW | 180 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Preservative | No Preservative |
| Purity | Antigen Affinity-purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
Flow | 01/31/2014 |
Summary
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reviewed by:
Samantha Garcia |
WB | Mouse | 11/06/2013 |
Summary
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reviewed by:
Verified Customer |
ICC | Other | 03/23/2010 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for NMDAR2B Antibody (NB300-106)Find related products by research area.
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| Verified Customer 01/31/2014 |
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| Application: | Flow | |
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| Samantha Garcia 11/06/2013 |
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| Application: | WB | |
| Species: | Mouse |
| Verified Customer 03/23/2010 |
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| Application: | ICC | |
| Species: | Other |
![Flow Cytometry: NMDAR2B Antibody [NB300-106] - NMDA2B receptor expression on CD4+ T cells. Image supplied by review from confirmed customer.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-Flow-Cytometry-NB300-106-img0004.jpg "Flow Cytometry: NMDAR2B Antibody [NB300-106] - NMDA2B receptor expression on CD4+ T cells. Image supplied by review from confirmed customer.")
![Western Blot: NMDAR2B Antibody [NB300-106]](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-Western-Blot-NB300-106-img0005.jpg "Western Blot: NMDAR2B Antibody [NB300-106]")
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